英文品名：Urea/Ammonia (Rapid) Assay Kit
规格型号：100 assays – 50 of each component
For the specific and rapid assay of urea and ammonia in water,beverages, milk and food products.
Urea and ammonia are widely occurring natural compounds. As urea is themost abundant organic solute in urine, and ammonia is produced as aconsequence of microbial protein catabolism, these analytes serve asreliable quality indicators for food products such as fruit juice, milk,cheese, meat and seafood. Ammonium carbonate is used as a leaven inbaked goods such as quick breads, cookies and muffins. Unlike some otherkits, this kit benefits from the use of a Gluta mate dehydrogenase thatis not inhibited by tannins found in, for example, grape juice and wine.In the wine industry, ammonia determination is important in thecalculation of yeast available nitrogen (YAN). YAN is comprised of threehighly variable components, free ammonium ions, primary amino nitrogen(from free amino acids) and the contribution from the sidechain ofL-Argi nine1. For the most accurate determination of YAN, all threecomponents should be quantified, and this is possible using Megazyme’s
L-Argi nine/Urea/Ammonia Kit (K-LARGE) and NOPA Kit
(K-PANOPA). Urea determination can be important in preventing theformation of the known carcinogen ethyl carbamate (EC) in finished wine.
Kits suitable for performing 50 assays (of each) are available fromMegazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (18 mL, pH 8.0) plus 2-oxoglutarate and
azide (0.02% w/v) as a preservative.
大于 2 years at 4°C.
Bottle 2: NADPH. Lyophilised powder.
Stable for 大于 5 years at -20°C.
Bottle 3: Gluta mate dehydrogenase suspension (1.1 mL).
大于 2 years at 4°C.
Bottle 4: Urease solution (2.7 mL).
大于 2 years at -20°C.
Bottle 5: Urea control powder (~ 2 g).
大于 2 years at room temperature.