lumiprobe荧光染料dsGreen Gel Staining Solution, 10000×

发表于2023年3月9日由上海金畔生物科技有限公司

lumiprobe荧光染料官网代理商水溶性荧光染料代理商脂溶性荧光染料代理商 dsGreen Gel Staining Solution, 10000×

PCR and qPCR

Bulk inquiry dsGreen Gel Staining Solution, 10000×

Cat. #	Quantity	Price
A0010	50 uL	$29.00
10010	0.5 mL	$100.00
20010	1 mL	$180.00

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dsGreen, an equivalent of SYBR^® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.

Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.

Comparison between ethidium bromide and dsGreen

Feature	Ethidium Bromide	dsGreen
Fluorescence	Red (615 nm)	Green (524 nm)
Excitation maximum	302 nm	454 nm
Excitation light source	UV only	Blue light or UV
Sensitivity	2 ng / band (dsDNA) 100 ng / band (RNA)	0.08 ng / band (dsDNA) 1–2 ng / band (oligonucleotides)
Health hazard	High	Low

Note: Fluorescence properties of SYBR Green I bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.