0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein （<0.01 ng/µg of the protein） as determined by the LAL test.
The LEAF™ （Low Endotoxin, Azide-Free） antibody was purified by affinity chromatography.
Storage & Handling:
The CD3 antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
FC – Quality tested IHC, Activ – Reported in the literature
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Clone OKT3 can block the binding of clones SK7 and UCHT1.8 The OKT3 antibody is able to induce T cell activation. Additional reported applications （for the relevant formats） include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody （Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered） is recommended for functional assays （Cat. No. 317304）. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody （Cat. No. 317326） with a lower endotoxin limit than standard LEAF™ purified antibodies （Endotoxin <0.01 EU/µg）.
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. 3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 4. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed 5. Alter G, et al. 2008. J. Virol. 82:9668. PubMed 6. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed 7. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed 8. Li B, et al. 2005. Immunology 116:487.
Human peripheral blood lymphocytes stained with LEAF™ purified OKT3, followed by anti-mouse IgG FITC
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CD3ε is a 20 kD chain of the CD3/T-cell receptor （TCR） complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ （CD247）, and a T-cell receptor （α/β or γ/δ） heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ （CD247） and TCR （α/β or γ/δ） forms CD3/TCR complex, 20 kD
Mature T and NK-T cells, thymocyte differentiation
Antigen recognition, signal transduction, T cell activation
Peptide antigen bound to MHC
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501.