QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

详细介绍

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

纯化得到至多10 mg的无内毒素高转染级纯质粒或柯斯质粒DNA

  • 纯化得到的DNA内毒素水平低于0.1 EU/µg
  • 纯化流程快,同时去除内毒素
  • 获得高产量、高拷贝质粒DNA
  • 使用LyseBlue,获得的裂解和高产量的DNA

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

质粒纯化方法与转染效率。

使用两种方法独立制备pRSVcat,分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中,及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit(100%)制备的DNA的转染效率表示。

质粒纯化方法与转染效率。

使用两种方法独立制备pRSVcat,分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中,及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit(100%)制备的DNA的转染效率表示。

相关产品订购信息:

EndoFree Plasmid Maxi Kit (10) 3890 12362 10
EndoFree Plasmid Mega Kit (5) 4720 12381 5
EndoFree Plasmid Giga Kit (5) 8080 12391 5
QIAGEN Large-Construct Kit (10) 4850 12462 10
HiSpeed Plasmid Midi Kit (25) 3840 12643 25
HiSpeed Plasmid Maxi Kit (10) 3180 12662 10
HiSpeed Plasmid Maxi Kit (25) 7440 12663 25
CompactPrep Plasmid Midi Kit (25) 3010 12843 25
CompactPrep Plasmid Maxi Kit (25) 6120 12863 25
QIAGEN Plasmid Plus Midi Sample Kit (5) 723 12941  
QIAGEN Plasmid Plus Midi Kit (25) 3600 12943 25
QIAGEN Plasmid Plus Midi Kit (100) 13240 12945  
QIAGEN Plasmid Plus Maxi Sample Kit (5) 1490 12961  
QIAGEN Plasmid Plus Maxi Kit (25) 7330 12963 25
QIAGEN Plasmid Plus Maxi Kit (100) 27180 12965  
QIAGEN Plasmid Plus Mega Kit (5) 3980 12981 5
QIAGEN Plasmid Plus Giga Kit (5) 6950 12991  
QIAGEN Blood&CellCulture DNA MiniKit(25) 3390 13323 25
Blood & Cell Culture DNA Midi Kit (25) 5400 13343 25
QIAGEN Blood&CellCulture DNA MaxiKit(10) 5070 13362 10
QIAGEN Plasmid Plus 96 Miniprep Kit (4) 35860 16181 384
QIArack 1250 19015  
Plasmid Buffer Set 1410 19046  
EndoFree Plasmid Buffer Set 7430 19048  
Buffer P1 (500 ml) 811 19051 500
Buffer P2 (500ml) 811 19052 500
Buffer P3 (500ml) 811 19053 500
Buffer QBT (1000ml) 1410 19054 1000
Buffer QC (1000ml) 1410 19055 1000
Buffer QF (1000ml) 1410 19056 1000
Genomic DNA Buffer Set 2080 19060 75
Buffer QG (250 ml) 666 19063 250
Buffer N3 (500ml) 1060 19064 500
Buffer PE (concentrate, 100ml) 1060 19065 100
Buffer PB (500ml) 1060 19066 500
Buffer AW/AW2 (concentrate, 324 ml) 1220 19072 324
Buffer AVL (155 ml) 1900 19073  
Buffer AL 1220 19075 1
Buffer ATL (200ml) 1220 19076 200
Buffer AE (240ml) 1220 19077 1
Buffer AW1 (concentrate, 242 ml) 1220 19081 1
Buffer ASL (4 x 140 ml) 1370 19082  
Buffer PM (500ml) 837 19083 500
Buffer EB (250ml) 423 19086 250
Reagent DX 663 19088 1
Buffer RPW (125ml) 598 19090 125
Buffer MTL (54 ml) 466 19112 54
QIAGEN Proteinase K (2ml) 1180 19131 2
QIAGEN Proteinase K (10 ml) 4200 19133 1
QIAGEN Protease (7.5 AU) 996 19155  
QIAGEN Protease (30 AU) 3200 19157 30
Collection Tubes (2-ml) 1830 19201  
VacConnectors (500) 1820 19407  
VacValves (24) 1250 19408  
QIAvac 24 Plus 4300 19413  
QIAvac Holder 1990 19418  
QIAvac Connecting System 13950 19419  
QIAvac 96 10200 19504  
Collection Microtubes (racked, 10 x 96) 2120 19560  
Collection Microtube Caps (120 x 8) 439 19566  
Tape Pads (5) 822 19570 5
QIAEX II Gel Extraction Kit (150) 2340 20021 150
QIAEX II Gel Extraction Kit (500) 5350 20051 500
QIAEX II Suspension (1.5ml) 1840 20902 1
Buffer QX1 (500ml) 1110 20912 500
R.E.A.L. Prep 96 Plasmid Kit (24) 54800 26173 2304
QIAprep Spin Miniprep Kit (50) 1070 27104 50
QIAprep Spin Miniprep Kit (250) 4100 27106 250
QIAprep 2.0 Spin Columns (100) 1130 27115 100
QIAprep 96 Turbo Core Kit (24) 64690 27173 2304
QIAprep 96 Turbo Miniprep Kit (4) 13660 27191 384
QIAprep 96 Turbo Miniprep Kit (24) 77320 27193 2304
QIAprep 96 Plus Miniprep Kit (4) 15610 27291 384
DirectPrep 96 Miniprep Kit (4) 7650 27361 384
QuickLyse Miniprep Kit (100) 2420 27405 100
QuickLyse Miniprep Kit (250) 5190 27406 250
MinElute PCR Purification Kit (50) 1330 28004 50
MinElute PCR Purification Kit (250) 6000 28006 250
MinElute 96 UF PCR Purification Kit (4) 3710 28051 384
MinElute 96 UF PCR Purification Kit (24) 14740 28053 2304
QIAquick PCR Purification Kit (50) 1100 28104 50
QIAquick PCR Purification Kit (250) 5150 28106 250
QIAquick 96 PCR Purification Kit (4) 10180 28181 384
QIAquick 96 PCR Purification Kit (24) 40340 28183 2304
MinElute Reaction Cleanup Kit (50) 1600 28204 50
MinElute Reaction Cleanup Kit (250) 7340 28206 250
QIAquick Nucleotide Removal Kit (50) 1410 28304 50
QIAquick Nucleotide Removal Kit (250) 6610 28306 250
MinElute Gel Extraction Kit (50) 1550 28604 50
MinElute Gel Extraction Kit (250) 7120 28606 250
QIAquick Gel Extraction Kit (50) 1100 28704 50
QIAquick Gel Extraction Kit (250) 5150 28706 250
Strep-Tactin?Superflow Plus (2 ml) 2340 30002 2
Strep-Tactin?Superflow Plus (10 ml) 9160 30004 10
Strep-Tactin Superflow Plus Cartrdg(1ml) 1980 30020 1
Strep-Tactin Superflow Plus Cartrdg(5ml) 6370 30060 5
Ni-NTA Agarose (25 ml) 3850 30210 25
Ni-NTA Agarose (100 ml) 13370 30230 100
Ni-NTA Agarose (500 ml) 56230 30250 500
NTA Agarose (25ml) 3950 30310 25
Ni-NTA Superflow (25 ml) 4740 30410 25
Ni-NTA Superflow (100 ml) 15920 30430 100
Ni-NTA Superflow (500 ml) 67410 30450 500
Ni-NTA Fast Start Kit (6) 4350 30600 6
Ni-NTA Superflow Columns (12 x 1.5 ml) 5200 30622 12
Ni-NTA Superflow Cartridges (5×1 ml) 2210 30721 5
Ni-NTA Superflow Cartridges (1×5 ml) 1960 30760 1
Ni-NTA Superflow Cartridges (5×5 ml) 7670 30761 5
Ni-NTA Superflow Cartridges (100×5 ml) 122510 30765 100
QIAamp UCP Pathogen Mini Kit (50) 4370 50214 50
QIAamp DNA Blood Mini Kit (50) 1770 51104 50
QIAamp DNA Blood Mini Kit (250) 7530 51106 250
QIAamp DNA Blood Mini QIAcube Kit (240) 8450 51126 240
QIAamp 96 DNA Blood Kit (4) 14930 51161 384
QIAamp 96 DNA Blood Kit (12) 41740 51162 384
QIAamp DNA Blood Midi Kit (20) 2590 51183 20
QIAamp DNA Blood Midi Kit (100) 11220 51185 100
QIAamp DNA Blood Maxi Kit (10) 2590 51192 10
QIAamp DNA Blood Maxi Kit (50) 10880 51194 50
FlexiGene DNA Kit (250) 3750 51206 250
FlexiGene DNA AGF3000 Kit (640) 49630 51297 640
QIAamp DNA Mini Kit (50) 2010 51304 50
QIAamp DNA Mini Kit (250) 8890 51306 250
QIAamp DNA Mini QIAcube Kit (240) 9340 51326 240
QIAamp 96 DNA QIAcube HT Kit (5) 8790 51331 480
QIAamp Fast DNA Tissue Kit, 50 1940 51404 50
QIAamp DNA Stool Mini Kit (50) 2870 51504 50
QIAamp Fast DNA Stool Mini Kit (50) 3190 51604 50
QIAamp DNA Microbiome Kit (50) 6620 51704 50
QIAamp RNA Blood Mini Kit (50) 4250 52304 50
QIAamp Viral RNA Mini Kit (50) 3010 52904 50
QIAamp Viral RNA Mini Kit (250) 12980 52906 250
QIAamp Viral RNA Mini QIAcube Kit (240) 15660 52926 240
QIAamp UltraSens Virus Kit (50) 6030 53704 50
QIAamp UltraSens Virus Kit (250) 25230 53706 250
QIAamp cador Pathogen Mini Kit (50) 2560 54104 50
QIAamp cador Pathogen Mini Kit (250) 12210 54106 250
cador Pathogen 96 QIAcube HT Kit (5) 11880 54161 480
QIAamp Circulating Nucleic Acid Kit (50) 14160 55114 50
QIAamp DNA Micro Kit (50) 2830 56304 50
QIAamp DNA FFPE Tissue Kit (50) 2590 56404 50
QIAamp DNA Investigator Kit (50) 2990 56504 50
QIAamp MinElute Media Kit (50) 4860 57414 50
QIAamp MinElute Virus Spin Kit (50) 3180 57704 50
QIAamp MinElute Virus Vacuum Kit (50) 4060 57714 50
QIAamp 96 Virus QIAcube HT Kit (5) 16960 57731 480
EpiTect Bisulfite Kit (48) 2800 59104 48
EpiTect 96 Bisulfite Kit (2) 9990 59110 192
EpiTect Plus DNA Bisulfite Kit (48) 2250 59124 48
EpiTect Whole Bisulfitome Kit (25) 4560 59203 25
EpiTect MSP Kit (100) 3600 59305 100
EpiTect MethyLight PCR Kit (200) 10340 59436 200
EpiTect HRM PCR Kit (100) 1440 59445 100
EpiTect MethyLight PCR +ROX Kit (200) 10600 59496 200
EpiTect Control DNA (1000) 2730 59568 1000
EpiTect Control DNA, methylated (100) 2730 59655 100
EpiTect Control DNA, unmethylated (100) 2730 59665 100
EpiTect PCR Control DNA Set (100) 5460 59695 300
EpiTect Fast 96 DNA Bisulfite Kit 10910 59720 192
EpiTect Fast 96 FFPE Bisulfite Kit 19310 59740 192
EpiTect Fast Bisulfite Kit (10) 847 59802 10
EpiTect Fast DNA Bisulfite Kit (50) 2430 59824 50
EpiTect Fast DNA Bisulfite Kit (200) 8290 59826 200
EpiTect Fast FFPE Bisulfite Kit (50) 4240 59844 50
EpiTect Fast LyseAll Bisulfite Kit (50) 2720 59864 50
EpiTect Fast LyseAll Bisulfite Kit (200) 9270 59866 200
QIAamp DSP Virus Kit (50),CE 4520 60704 50
QIAamp DSP DNA Blood Mini Kit (50), CE 2530 61104 50
QIAamp DSP Virus Spin Kit (50) 3580 61704 50
MagAttract HMW DNA Kit (48) 3970 67563 48
DNeasy Plant Maxi Kit (24) 4590 68163 24
DNeasy Plant Mini Kit (50) 2750 69104 50
DNeasy Plant Mini Kit (250) 12310 69106 250
DNeasy 96 Plant Kit (6) 11590 69181 576
DNeasy Blood & Tissue Kit (50) 2140 69504 50
DNeasy Blood & Tissue Kit (250) 8810 69506 250
DNeasy mericon Food Kit (50) 1990 69514 50
DNeasy mericon 96 QIAcube HT Kit (5) 11430 69571 480
DNeasy 96 Blood & Tissue Kit (4) 8780 69581 384
DNeasy 96 Blood & Tissue Kit (12) 24240 69582 1152
TissueLyser Single-Bead Dispenser, 5mm 3520 69965  
TissueLyser Single-Bead Dispenser, 7mm 3520 69967  
TissueLyser 3 mm Bead Dispenser, 96-well 10160 69973  
TissueLyser 5 mm Bead Dispenser, 96-well 10160 69975  
TissueLyser LT Adapter, 12-Tube 17250 69980  
TissueLyser Adapter Set (2 x 24) 16270 69982  
TissueLyser Adapter Set (2 x 96) 12200 69984  
RNeasy Protect Animal Blood Kit (50) 6580 73224 50
Rneasy Microarray Tissue Mini Kit (50) 5350 73304 50
RNeasy Plus Universal Tissue Mini Kit50 5330 73404 50
RNeasy Plus Universal Tissue Midi Kit10 2820 73442 10