qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit

详细介绍

qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit

亚硫酸氢盐的完全转化和DNA回收,用于甲基化分析

  • 简化的流程,获得快速、可靠的结果
  • 胞嘧啶完全转化
  • 独特的DNA保护体系,用于高度敏感的分析
  • 优化的试验方案可处理FFPE组织样本DNA
  • 离心柱和高通量96孔板方式
  • EpiTect Bisulfite Kit可在6小时内将DNA 序列中非甲基化的胞嘧啶完全转化为尿嘧啶。EpiTect 96 Bisulfite操作时间少于7小时。高灵敏度的方法采用全新的保护机制防止DNA降解,确保即使是1 ng的DNA也可获得灵敏的结果,转化率可高达99%。得益于独特的DNA保护技术,EpiTect Bisulfite转化后DNA高度适用于EpiTect Whole Bisulfitome Kits转化后全基因组扩增。因此可在转化后DNA量较少的情况下确保可靠的扩增。EpiTect Bisulfite Kits包括离心柱或96孔板形式的扩增和脱磺酸基过程,确保快速可靠的实验结果用于下游应用。两个试剂盒都可用于离心机或真空装置,另外,EpiTect Bisulfite Kit可在QIAcube全自动核酸纯化仪上全自动运行。

qiagen 59104 甲基化转化试剂盒EpiTect Bisulfite Kit

Performance

快速得到实验结果

完整的EpiTect离心和真空过程只需6小时,包括从DNA到待分析的亚硫酸氢盐转化后的高纯DNA。EpiTect 96 Bisulfite操作时间少于7小时。传统的亚硫酸氢盐转化过程需要18个小时,且需要大量的操作(参见”快速简单的亚硫酸氢盐转化”和”Time saving procedure”)。EpiTect Bisulfite Kit为成功进行亚硫酸氢盐转化和DNA回收提供所需的全部试剂。

快速简单的亚硫酸氢盐转化
  EpiTect流程 传统流程
反应构建 10分钟;预制溶液 40分钟;需反应构建
亚硫酸氢盐转化 5小时 16小时
纯化 30–45分钟; EpiTect离心柱或孔板 120分钟;纯化、脱璜、沉淀和洗涤

订购信息:

EpiTect Bisulfite Kit (48) 2800 59104
EpiTect 96 Bisulfite Kit (2) 9990 59110
EpiTect Plus DNA Bisulfite Kit (48) 2250 59124
EpiTect Whole Bisulfitome Kit (25) 4560 59203
EpiTect MSP Kit (100) 3600 59305
EpiTect MethyLight PCR Kit (200) 10340 59436
EpiTect HRM PCR Kit (100) 1440 59445
EpiTect MethyLight PCR +ROX Kit (200) 10600 59496
EpiTect Control DNA (1000) 2730 59568
EpiTect Control DNA, methylated (100) 2730 59655
EpiTect Control DNA, unmethylated (100) 2730 59665
EpiTect PCR Control DNA Set (100) 5460 59695
EpiTect Fast 96 DNA Bisulfite Kit 10910 59720
EpiTect Fast 96 FFPE Bisulfite Kit 19310 59740
EpiTect Fast Bisulfite Kit (10) 847 59802
EpiTect Fast DNA Bisulfite Kit (50) 2430 59824
EpiTect Fast DNA Bisulfite Kit (200) 8290 59826
EpiTect Fast FFPE Bisulfite Kit (50) 4240 59844
EpiTect Fast LyseAll Bisulfite Kit (50) 2720 59864
EpiTect Fast LyseAll Bisulfite Kit (200) 9270 59866
QIAamp DSP Virus Kit (50),CE 4520 60704
QIAamp DSP DNA Blood Mini Kit (50), CE 2530 61104
QIAamp DSP Virus Spin Kit (50) 3580 61704
MagAttract HMW DNA Kit (48) 3970 67563
DNeasy Plant Maxi Kit (24) 4590 68163
DNeasy Plant Mini Kit (50) 2750 69104
DNeasy Plant Mini Kit (250) 12310 69106
DNeasy 96 Plant Kit (6) 11590 69181
DNeasy Blood & Tissue Kit (50) 2140 69504
DNeasy Blood & Tissue Kit (250) 8810 69506
DNeasy mericon Food Kit (50) 1990 69514
DNeasy mericon 96 QIAcube HT Kit (5) 11430 69571
DNeasy 96 Blood & Tissue Kit (4) 8780 69581
DNeasy 96 Blood & Tissue Kit (12) 24240 69582
TissueLyser Single-Bead Dispenser, 5mm 3520 69965
TissueLyser Single-Bead Dispenser, 7mm 3520 69967
TissueLyser 3 mm Bead Dispenser, 96-well 10160 69973
TissueLyser 5 mm Bead Dispenser, 96-well 10160 69975
TissueLyser LT Adapter, 12-Tube 17250 69980
TissueLyser Adapter Set (2 x 24) 16270 69982
TissueLyser Adapter Set (2 x 96) 12200 69984
RNeasy Protect Animal Blood Kit (50) 6580 73224
Rneasy Microarray Tissue Mini Kit (50) 5350 73304
RNeasy Plus Universal Tissue Mini Kit50 5330 73404
RNeasy Plus Universal Tissue Midi Kit10 2820 73442
RNeasy FFPE Kit (50) 5810 73504
RNeasy Micro Kit (50) 6600 74004
RNeasy Plus Micro Kit (50) 5720 74034
RNeasy Mini Kit (50) 4160 74104
RNeasy Mini Kit (250) 17520 74106
RNeasy Mini QIAcube Kit (240) 19280 74116
RNeasy Protect Mini Kit (50) 5000 74124
RNeasy Protect Mini Kit (250) 21950 74126
RNeasy Plus Mini Kit (50) 4760 74134
RNeasy Plus Mini Kit (250) 20060 74136
RNeasy 96 QIAcube HT Kit (5) 14870 74171
RNeasy 96 Kit (4) 21090 74181
RNeasy 96 Kit (12) 57970 74182
Rneasy Plus 96 Kit (12) 66810 74192
RNeasy MinElute Cleanup Kit (50) 4720 74204
RNeasy Protect Saliva Mini Kit (50) 9920 74324
RNeasy FFPE Kit (50) 5940 74404
RNeasy Protect Bacteria Mini Kit (50) 6570 74524
RNeasy Protect Cell Mini Kit (50) 5000 74624
RNeasy Fibrous Tissue Mini Kit (50) 5420 74704
RNeasy Lipid Tissue Mini Kit (50) 5430 74804
RNeasy 96 Universal Tissue Kit (4) 26280 74881
RNeasy Plant Mini Kit (20) 2090 74903
RNeasy Plant Mini Kit (50) 4860 74904
RNeasy Midi Kit (10) 2070 75142
RNeasy Midi Kit (50) 9230 75144
RNeasy Maxi Kit (12) 4900 75162
exoEasy Maxi Kit (20) 6720 76064
RNAlater (50ml) 1140 76104