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6-fluorescein (FAM) derivative of deoxycytidine triphosphate (dCTP).
FAM 6-isomer is a common fluorophore used for labeling biomolecules; FAM has a high quantum yield and bright green emission.
FAM-11-dCTP can be used for enzymatic DNA labeling via Nick-translation, 3’-end labeling, random primed labeling, PCR, or cDNA synthesis. FAM-11-dCTP is incorporated into the growing DNA chain by Taq-polymerase, DNA-polymerase I, Phi 29 polymerase, Klenow fragment, reverse transcriptase, or terminal transferase.
The linker length (11 carbon atoms) is optimal for the efficient incorporation of fluorescently-labeled dCTP into the growing DNA chain.
Labeled DNA can be used for FISH experiments, microarray gene profiling, Southern blot, and Northern blot.
Absorption and emission spectra of FAM
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FAM-11-dUTP, 6-isomer
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