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R6G (rhodamine 6G) is a yellow emitting xanthene dye. It has been used for DNA sequencing before. Now this dye is often used for qPCR. This is a pure 5-isomer of R6G with azide group.
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Absorption and emission spectra for 5-R6G
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Click-Chemistry Labeling of Oligonucleotides and DNA
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THPTA ligand
THPTA is a water soluble ligand for Cu(I) catalyzed Click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.