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Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, PEP fluorescence is more red-shifted
PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.
This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and Click Chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.
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Spectra of PEP (phenylethynylpyrene) in ethanol
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BDP 581/591 amine
BDP 581/591 fluorophore derivative with free amine group for the conjugation with electrophilic reagents, and enzymatic transamination.
