MES monohydrate Buffer蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

MES monohydrate Buffer
MES monohydrate Buffer

MES monohydrate Buffer

MES monohydrate Buffer

MES monohydrate Buffer

MES monohydrate Buffer

Products > Optimize Reagents > Optimize – Buffers > MES monohydrate Buffer

MES monohydrate Buffer

Applications

  • Crystallization grade MES monohydrate for formulating screens or for optimization

Features

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

MES monohydrate

Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate
C6H13NO4S • H2O
Mr 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 329824236
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity ≥ 99.0%
pKa 6.1 at 25°C

Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.

Useful pH range of MES 5.2 – 7.1

Titrate HR2-587 to the desired pH using NaOH
HR2-587 Measured pH Range: 3.2 – 3.4 at 25°C
HR2-587 Measured Conductivity Range: 166.4 – 2440 µS/cm at 25°C
HR2-587 Measured Refractive Index Range: 1.34655 – 1.34731 at 20°C

HR2-787 has been titrated to pH 6.5 using NaOH
HR2-787 Measured Conductivity Range: 19.2 – 25.6 mS/cm at 25°C
HR2-787 Measured Refractive Index Range: 1.36113 – 1.36251 at 20°C

HR2-943-07 – MES monohydrate pH 5.8 – 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.

Impurities:
Endotoxin ≤ 50 EU/g
Yeast and Mold ≤ 100 CFU/g
Bioburden ≤ 100 CFU/g
Al ≤ 5.0 ppm
As ≤ 3.0 ppm
Ba ≤ 5.0 ppm
Bi ≤ 5.0 ppm
Ca ≤ 20.0 ppm
Cd ≤ 5.0 ppm
Co ≤ 5.0 ppm
Cr ≤ 5.0 ppm
Cu ≤ 5.0 ppm
Fe ≤ 5.0 ppm
Li ≤ 5.0 ppm
Mg ≤ 5.0 ppm
Mn ≤ 5.0 ppm
Mo ≤ 5.0 ppm
Na ≤ 20.0 ppm
Ni ≤ 5.0 ppm
Pb ≤ 5.0 ppm
Sb ≤ 5.0 ppm
Zn ≤ 5.0 ppm

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MES monohydrate Buffer

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MES monohydrate Buffer

CAT NO

HR2-587

NAME

0.5 M MES monohydrate

DESCRIPTION

100 mL

PRICE

$129.00

cart quote

CAT NO

HR2-787

NAME

1.0 M MES monohydrate pH 6.5

DESCRIPTION

100 mL

PRICE

$129.00

cart quote

Support Material(s)

MES monohydrate Buffer HR2-587 MES monohydrate 0.5 M solution SDSMES monohydrate Buffer HR2-787 MES monohydrate pH 6.5 1.0 M solution SDSMES monohydrate Buffer Titration Table

Certificate Of Analysis

Related Item(S)

  • Sodium hydroxide
  • StockOptions MES Buffer Kit
  • Individual StockOptions MES Reagents

References

1. Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. Biochemistry 33, 1555- 1564, 1994.

MES monohydrate Buffer MES monohydrate Buffer

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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