Magazyme试剂盒Cellulase Assay Kit CellG3 Method K-CellG3 CellG3

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Cellulase Assay Kit (CellG3 Method) Cellulase Assay Kit CellG3 Method K-CellG3 CellG3 Cellulase Assay Kit CellG3 Method K-CellG3 CellG3 Cellulase Assay Kit CellG3 Method K-CellG3 CellG3

Product code: K-CellG3


180 / 360 assays per kit

Prices exclude VAT

  • Assay Protocol
  • Content: 180 / 360 assays per kit
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: endo-Cellulase
    Assay Format: Spectrophotometer, Auto-analyser
    Detection Method: Absorbance
    Wavelength (nm): 400
    Signal Response: Increase
    Limit of Detection: 0.05 U/mL
    Reproducibility (%): ~ 3%
    Total Assay Time: ~ 20 min
    Application examples: Fermentation broths, industrial enzyme preparations and biofuels research.
    Method recognition: Novel method

    The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 
    1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase.  The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase.  The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

    Please note that a new assay kit (K-CellG5) is now available for the measurement of endo-cellulase.  The CellG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases.  In addition, the exchange of the benzylidene blocking group in CellG3 for 3-keto-butylidene in CellG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay.  As DMSO is known to inhibit certain cellulases, this is another benefit in using CellG5.  Megazyme now recommends the use of K-CellG5 for all assays for the measurement of endo-cellulase.

    Browse more cellulase and other enzyme activity assay kits.

    Cellulase Assay Kit CellG3 Method K-CellG3 CellG3

    • Very cost effective 
    • All reagents stable for > 2 years after preparation 
    • Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested 
    • Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6 
    • Simple format. Well suited to automation  
    • Standard included
  • Related products
    Similar Products
    K-CellG5-2V – Cellulase Assay Kit (CellG5 Method) T-CCZ-200T – Cellazyme C Tablets S-ACMCL – Azo-CM-Cellulose (Liquid)

    Related Carbohydrates
    P-CMC4M – Carboxymethyl Cellulose 4M P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-GLCML – Glucomannan (Konjac; Low Viscosity) P-GLCMH – Glucomannan (Konjac; High Viscosity) P-XYGLN – Xyloglucan (Tamarind) O-CTR-50MG – Cellotriose O-CTE-50MG – Cellotetraose O-CPE-20MG – Cellopentaose O-CHE – Cellohexaose

    Associated Enzymes
    E-CELAN – Cellulase (endo-1,4-β-D-glucanase)
    (Aspergillus niger) E-CELBA – Cellulase (endo-1,4-β-D-glucanase)
    (Bacillus amyloliquefaciens) E-CELTE – Cellulase (endo-1,4-β-D-glucanase)
    (Talaromyces emersonii) E-CELTH – Cellulase (endo-1,4-β-D-glucanase)
    (Thermobifida halotolerans) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
    (Trichoderma longibrachiatum) E-CELTM – Cellulase (endo-1,4-β-D-glucanase)
    (Thermotoga maritima)

    Certificate of Analysis

    Safety Data Sheet

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    Safety Data Sheet

    Cellulase Assay Kit CellG3 Method K-CellG3 CellG3