Mezazyme酶试剂盒中国代理商 欢迎访问Megazyme试剂盒官网了解更多信息
Cellulase Assay Kit (CellG3 Method)
180 / 360 assays per kit
Prices exclude VAT
Content: | 180 / 360 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | endo-Cellulase |
Assay Format: | Spectrophotometer, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 400 |
Signal Response: | Increase |
Limit of Detection: | 0.05 U/mL |
Reproducibility (%): | ~ 3% |
Total Assay Time: | ~ 20 min |
Application examples: | Fermentation broths, industrial enzyme preparations and biofuels research. |
Method recognition: | Novel method |
The CellG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CellG5) is now available for the measurement of endo-cellulase. The CellG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases. In addition, the exchange of the benzylidene blocking group in CellG3 for 3-keto-butylidene in CellG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay. As DMSO is known to inhibit certain cellulases, this is another benefit in using CellG5. Megazyme now recommends the use of K-CellG5 for all assays for the measurement of endo-cellulase.
Browse more cellulase and other enzyme activity assay kits.
- Very cost effective
- All reagents stable for > 2 years after preparation
- Completely specific for cellulase (endo-1,4-glucanase). The substrate is not hydrolysed by β-glucosidase, cellbiohyrolase or any other enzymes tested
- Kinetic assays possible due to significant phenolate ion presence (and UV absorbance) at pH 5-6
- Simple format. Well suited to automation
- Standard included
(Aspergillus niger) E-CELBA – Cellulase (endo-1,4-β-D-glucanase)
(Bacillus amyloliquefaciens) E-CELTE – Cellulase (endo-1,4-β-D-glucanase)
(Talaromyces emersonii) E-CELTH – Cellulase (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
(Trichoderma longibrachiatum) E-CELTM – Cellulase (endo-1,4-β-D-glucanase)
(Thermotoga maritima)
FAQs Assay Protocol Data Calculator
Symbol : | Not Applicable |
Signal Word : | Not Applicable |
Hazard Statements : | Not Applicable |
Precautionary Statements : | Not Applicable |