Magazyme试剂盒Cellulase Assay Kit CellG5 Method K-CellG5 s

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Cellulase Assay Kit (CellG5 Method) Cellulase Assay Kit CellG5 Method K-CellG5 s Cellulase Assay Kit CellG5 Method K-CellG5 s

Product code: K-CellG5-2V



60 / 120 assays (manual) / 240 assays (auto-analyser)

Prices exclude VAT

  • Assay Protocol
  • Content: (K-CellG5-2V)
    60 / 120 assays (manual) / 240 assays (auto-analyser) or 

    120 / 240 assays (manual) / 480 assays (auto-analyser)
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: endo-Cellulase
    Assay Format: Spectrophotometer, Auto-analyser
    Detection Method: Absorbance
    Wavelength (nm): 400
    Signal Response: Increase
    Limit of Detection: 1.2 x 10-3 U/mL
    Reproducibility (%): ~ 3%
    Total Assay Time: 10 min
    Application examples: Fermentation broths, industrial enzyme preparations and biofuels research.
    Method recognition: Novel method

    Cellulase Activity Assay Kit.

    The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

    The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

    View Cellulase Activity Assay Protocol.

    View our complete list of assay kits for enzyme activities.

    Cellulase Assay Kit CellG5 Method K-CellG5 s

    • Very cost effective 
    • All reagents stable for > 4 years 
    • Completely specific for cellulase (endo-1,4-glucanase) 
    • Generally applicable and highly sensitive 
    • Simple format. Well suited to automation 
    • Standard included
  • Related products
    Similar Products
    K-CellG3 – Cellulase Assay Kit (CellG3 Method) T-CCZ-200T – Cellazyme C Tablets S-ACMCL – Azo-CM-Cellulose (Liquid)

    Related Carbohydrates
    P-CMC4M – Carboxymethyl Cellulose 4M P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-GLCML – Glucomannan (Konjac; Low Viscosity) P-GLCMH – Glucomannan (Konjac; High Viscosity) P-XYGLN – Xyloglucan (Tamarind) O-CTR-50MG – Cellotriose O-CTE-50MG – Cellotetraose O-CPE-20MG – Cellopentaose O-CHE – Cellohexaose

    Associated Enzymes
    E-CELAN – Cellulase (endo-1,4-β-D-glucanase)
    (Aspergillus niger) E-CELBA – Cellulase (endo-1,4-β-D-glucanase)
    (Bacillus amyloliquefaciens) E-CELTE – Cellulase (endo-1,4-β-D-glucanase)
    (Talaromyces emersonii) E-CELTH – Cellulase (endo-1,4-β-D-glucanase)
    (Thermobifida halotolerans) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
    (Trichoderma longibrachiatum) E-CELTM – Cellulase (endo-1,4-β-D-glucanase)
    (Thermotoga maritima)

    Certificate of Analysis

    Safety Data Sheet

    Assay Protocols

    Assay Protocol Data Calculator Validation Report

    Safety Information
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    Signal Word : Not Applicable
    Hazard Statements : Not Applicable
    Precautionary Statements : Not Applicable
    Safety Data Sheet

    Cellulase Assay Kit CellG5 Method K-CellG5 s