Magazyme试剂盒Pullulanase-Limit-Dextrinase Assay Kit PullG6 Method K-PullG6

Mezazyme酶试剂盒中国代理商 欢迎访问Megazyme试剂盒官网了解更多信息
Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method) Pullulanase-Limit-Dextrinase Assay Kit PullG6 Method K-PullG6 Pullulanase-Limit-Dextrinase Assay Kit PullG6 Method K-PullG6 Pullulanase-Limit-Dextrinase Assay Kit PullG6 Method K-PullG6

Product code: K-PullG6

€265.00

100/200 assays per kit

Prices exclude VAT

  • Assay Protocol
  • SDS
  • Mega-Calc
  • COAs
  • FAQs
  • Content: 100 / 200 assays per kit
    Shipping Temperature: Ambient
    Storage Temperature: Short term stability: 2-8oC,
    Long term stability: See individual component labels
    Stability: > 2 years under recommended storage conditions
    Analyte: Pullulanase/Limit-Dextrinase
    Assay Format: Spectrophotometer
    Detection Method: Absorbance
    Wavelength (nm): 400
    Signal Response: Increase
    Limit of Detection: 0.18 U/mL for pullulanase preparations (50-fold dilution)
    0.01 U/g for limit dextrinase in milled malt
    Reproducibility (%): ~ 3%
    Total Assay Time: ~ 10 min (Pullanase),
    ~ 30 min (Limit-Dextrinase)
    Application examples: Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
    Method recognition: Novel method

    PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

    Explore more of our assay kit products for enzyme activity measurement.

    Pullulanase-Limit-Dextrinase Assay Kit PullG6 Method K-PullG6

    Advantages
    • High sensitivity 
    • Suitable for manual and auto-analyser formats 
    • No transglycosylation interference 
    • Very cost effective 
    • All reagents stable for > 1 year after preparation 
    • Very specific
    • Simple format
    • Standard included

    Related products
    Related Carbohydrates
    P-PULLN – Pullulan P-BLDX-10G – β-Limit Dextrin O-MTMT – 63-α-D-Maltotriosyl-maltotriose O-GMH – 63-α-D-Glucosyl-maltotriosyl-maltotriose

    Colourimetric Substrates
    T-LDZ-200T – Limit-Dextrizyme Tablets I-AZPUL – AZCL-Pullulan

    Associated Enzymes
    E-PULKP – Pullulanase M1 (Klebsiella planticola) E-PULBL – Pullulanase M2 (Bacillus licheniformis)

    Related Products
    D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer) K-AMYLSD – α-Amylase SD Assay Kit K-BETA3 – β-Amylase Assay Kit (Betamyl-3) R-AMGR3 – Amyloglucosidase Assay Reagent O-PNPAG – 4-Nitrophenyl-α-D-glucopyranoside

    Related Carbohydrates
    P-PULLN – Pullulan P-BLDX-10G – β-Limit Dextrin O-MTMT – 63-α-D-Maltotriosyl-maltotriose O-GMH – 63-α-D-Glucosyl-maltotriosyl-maltotriose
    Colourimetric Substrates
    T-LDZ-200T – Limit-Dextrizyme Tablets I-AZPUL – AZCL-Pullulan
    Associated Enzymes
    E-PULKP – Pullulanase M1 (Klebsiella planticola) E-PULBL – Pullulanase M2 (Bacillus licheniformis)
    Related Products
    D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer) K-AMYLSD – α-Amylase SD Assay Kit K-BETA3 – β-Amylase Assay Kit (Betamyl-3) R-AMGR3 – Amyloglucosidase Assay Reagent O-PNPAG – 4-Nitrophenyl-α-D-glucopyranoside

    Pullulanase-Limit-Dextrinase Assay Kit PullG6 Method K-PullG6