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Protease (Subtilisin A from Bacillus licheniformis)
Product code: E-BSPRT-40ML
€217.00
2 grams – 40ML
Prices exclude VAT
Content: | 0.5 grams – 10 mL or 2 grams – 40 mL or 5 grams – 100 mL or 2.5 grams – 100 mL (ANKOM) |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 50% (v/v) glycerol |
Physical Form: | Solution |
Stability: | > 4 years at 4oC |
Enzyme Activity: | Protease |
EC Number: | 3.4.21.62 |
CAS Number: | 9014-01-1 |
Synonyms: | subtilisin; subtilisin A |
Source: | Bacillus licheniformis |
Molecular Weight: | 30,250 |
Expression: | Purified from Bacillus licheniformis |
Specificity: | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
Specific Activity: | ~ 6 U/mg of protein (40oC, pH 8.0 on casein) |
Unit Definition: | One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). |
Temperature Optima: | 60oC |
pH Optima: | 7 |
Application examples: | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. |
High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Total Dietary Fiber test method.
E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
Show all analytical enzymes products.
Data booklets for each pack size are located in the Documents tab.
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E-BSPRPD – Protease Subtilisin A (from Bacillus licheniformis) Powder E-PAPN – Papain (Carica papaya) E-BROM – Bromelain from pineapple stems
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(Ananas comosus)
Colourimetric Substrates
T-PRAK-200T – Protazyme AK Tablets T-PROL-200T – Protazyme OL Tablets S-AZCAS – Azocasein I-AZCAS – AZCL-Casein I-AZCOL – AZCL-Collagen
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E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
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K-PDCAAS – Protein Digestibility Assay Kit K-TDFR-200A – Total Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-INTDF – Integrated Total Dietary Fiber Assay Kit