Amplite 比色法Bradford蛋白定量分析试剂盒 货号11118-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Amplite 比色法Bradford蛋白定量分析试剂盒

Amplite 比色法Bradford蛋白定量分析试剂盒

货号 11118 存储条件 Multiple
规格 1000 Tests 价格 1176
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

传统的Bradford蛋白质检测法广泛用于定量蛋白质浓度。但是,许多市场的许多操作说明非常复杂繁琐。 我们的Amplite 比色法Braford蛋白定量检测试剂盒是一种用于测定总蛋白浓度的包含两组分且与洗涤剂兼容的测定方法。该检测法基于与Bradford蛋白质测定法相同的考马斯亮蓝G-250蛋白指示剂,并提供相当的准确性。我们专有的配方使我们的试剂盒更加方便快捷。在600 nm附近检测蛋白质信号,并在30分钟内完成测定。 Amplite 比色Braford蛋白定量分析试剂盒可以以方便的96孔板进行操作,无需分离步骤即可轻松实现自动化。

 

适用仪器


光吸收酶标仪  
吸收: 595nm
推荐孔板: 透明底板

产品说明书

样品分析方案

概述

准备Bradford工作溶液(50μL)
添加BSA标准品或检测样品(50μL)
在室温下孵育5-15分钟
读取595 nm处的吸光度

注:使用前,请在室温下解冻所有试剂盒组分。

 

标准溶液制备:

BSA标准溶液:

将20 µL的1 mg / mL BSA标准溶液(组分C)添加到480 µL的PBS(未提供)中,以生成40 µg / mL的BSA标准溶液(BS1)。然后在PBS中执行1:2连续稀释,以得到连续稀释的BSA标准BS2-BS7。注意:有必要为每个测定创建标准曲线。

  

操作步骤

表1.在透明的底96孔板中布置BSA标准品和测试样品。 BS = BSA标准品(BS1-BS7,40至0.625 µg / mL); BL =空白控件; TS =测试样品

BS1 BS1 TS TS
BS2 BS2
BS3 BS3    
BS4 BS4    
BS5 BS5    
BS6 BS6    
BS7 BS7    
BL BL    

表2:每个孔的试剂

溶液体积 试剂
BS1-BS7 50 µL 系列稀释液(40-0.625 µg / mL)
BL 50 µL PBS
TS 50 µL 测试样品

1.根据表1和表2提供的布局准备BSA标准(BS),空白对照(BL)和检测样品(TS)。
2.向BSA标准品,空白对照和测试样品的每个孔中添加50 µL的Bradford工作溶液,以使总测定体积为100 µL /孔。
3.将反应在室温下孵育5至15分钟。
4.用酶标仪在OD 595 nm处读取吸光度。

 

图示

Amplite 比色法Bradford蛋白定量分析试剂盒   货号11118

图1.使用透明底96孔板,使用Amplite 比色法Bradford蛋白定量分析试剂盒测量BSA剂量响应。

  

参考文献

The Effects of a Concentrated Surfactant Gel on Biofilm EPS.
Authors: Salisbury, Anne-Marie and Chen, Rui and Mullin, Marc and Foulkes, Lauren and Percival, Steven L
Journal: Surgical technology international (2020): 31-35

The effect of Chinese wild blueberry fractions on the growth and membrane integrity of various foodborne pathogens.
Authors: Zhou, Tong-Tong and Wei, Cai-Hong and Lan, Wei-Qing and Zhao, Yong and Pan, Ying-Jie and Sun, Xiao-Hong and Wu, Vivian C H
Journal: Journal of food science (2020): 1513-1522

[Preliminary Investigation on Difference of Protein Compositions Between DC2.4 Cells and Their Derived Exosomes by nanoLC-MS/MS].
Authors: Lin, Qing and Li, Yan and Qu, Meng-Ke and Liu, Xing and Zhang, Zhi-Rong and Zhang, Ling
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2020): 81-86

Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions.
Authors: Zendrini, Andrea and Paolini, Lucia and Busatto, Sara and Radeghieri, Annalisa and Romano, Miriam and Wauben, Marca H M and van Herwijnen, Martijn J C and Nejsum, Peter and Borup, Anne and Ridolfi, Andrea and Montis, Costanza and Bergese, Paolo
Journal: Frontiers in bioengineering and biotechnology (2019): 452

Combined Fluorimetric Caspase-3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity.
Authors: Larsen, Anna K and Hall, Arnaldur and Lundsgart, Henrik and Moghimi, S Moein
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 301-311

Effect of nanoparticle size and PEGylation on the protein corona of PLGA nanoparticles.
Authors: Partikel, Katrin and Korte, Robin and Stein, Nora C and Mulac, Dennis and Herrmann, Fabian C and Humpf, Hans-Ulrich and Langer, Klaus
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2019): 70-80

Magnetic Nanoclusters Coated with Albumin, Casein, and Gelatin: Size Tuning, Relaxivity, Stability, Protein Corona, and Application in Nuclear Magnetic Resonance Immunoassay.
Authors: Khramtsov, Pavel and Barkina, Irina and Kropaneva, Maria and Bochkova, Maria and Timganova, Valeria and Nechaev, Anton and Byzov, Il’ya and Zamorina, Svetlana and Yermakov, Anatoly and Rayev, Mikhail
Journal: Nanomaterials (Basel, Switzerland) (2019)

Pilose Antler Extracts (PAEs) Protect against Neurodegeneration in 6-OHDA-Induced Parkinson’s Disease Rat Models.
Authors: Li, Chaohua and Sun, Yanan and Yang, Weifeng and Ma, Shuhua and Zhang, Lili and Zhao, Jing and Zhao, Xin and Wang, Yi
Journal: Evidence-based complementary and alternative medicine : eCAM (2019): 7276407

Rhubarb-Evoke Mucus Secretion through Aggregation and Degranulation of Mast Cell in the Colon of Rat: In vivo and ex vivo studies.
Authors: Wu, Di and Xue, Xiaowei and Gao, Chenchen and Liu, Yuehong and Wang, Tiantian and Li, Lisheng and Tong, Xuehong and Li, Feng and Xu, Jingdong
Journal: Scientific reports (2019): 19375

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles.
Authors: Partikel, Katrin and Korte, Robin and Mulac, Dennis and Humpf, Hans-Ulrich and Langer, Klaus
Journal: Beilstein journal of nanotechnology (2019): 1002-1015

说明书
Amplite 比色法Bradford蛋白定量分析试剂盒.pdf