新型钙离子荧光探针Calbryte-520XL AM（停产）

简要概述

产品基本信息

货号：20646

产品名称：新型钙离子荧光探针Calbryte-520XL AM

规格：10×50 ug

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：876.94

溶剂：水

激发波长(nm)：492

发射波长(nm)：514

产品介绍

新型钙离子荧光探针Calbryte-520XL AM是美国AAT Bioquest生产的钙离子荧光探针，Calbryte 520XL，AM是一种具有极低亲和力的新型荧光且可渗透细胞的钙指示剂。像其他染料AM细胞加载一样，Calbryte 520XL AM酯不发荧光，一旦进入细胞内部，它就会被细胞内酯酶水解并被激活。激活的指示剂是一种极性分子，不再能够自由扩散通过细胞膜，基本上被困在细胞内部。Calbryte 520XL对钙离子的亲和力低，Kd约为300 uM，这与众所周知的Rhod 5N相似，但比Rhod-5N稳定得多。Calbryte 520XL在高浓度钙离子存在下产生明亮的荧光信号。它具有与Fluo-4相同的激发和发射波长，因此相同的Fluo-4测定设置可轻松应用于基于Calbryte 520XL的钙测定。Calbryte 520XL是Rhod-5N的绝佳替代品。我们还提供Calbryte 520XL，钾盐（＃20645），Calbryte 520XL-右旋糖酐（＃20648），Calbryte 520XL叠氮化物（＃20643），它们可以通过众所周知的点击化学轻松地偶联到载体上。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的新型钙离子荧光探针Calbryte-520XL AM。 

点击查看光谱

点击查看实验方案

钙离子篇：时间轴式讲解应用于钙离子检测的探针

参考文献

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling Tang
Journal: The International Journal of Biochemistry & Cell Biology (2017)

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Jia Liu, Qing Du, He Zhu, Yu Li, Maodong Liu, Shoushui Yu, Shilei Wang
Journal: Int J Clin Exp Med (2017): 6861–6868

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Jiang Lu, Xue-qin Yao, Xin Luo, Yu Wang, Sookja Kim Chung, He-xin Tang, Chi Wai Cheung, Xian-yu Wang, Chen Meng, Qing Li
Journal: Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Journal: Scientific Reports (2017): 41781

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Xia Sun, Yi Lin, Qiansheng Huang, Junpeng Shi, Ling Qiu, Mei Kang, Yajie Chen, Chao Fang, Ting Ye, Sijun Dong
Journal: Journal of cellular and molecular medicine (2015): 581–594

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Lantao Zhao, Shuhong Li, Shilei Wang, Ning Yu, Jia Liu
Journal: Biochemical and biophysical research communications (2015): 537–542

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Xu-Dong Peng, Gui-Qiu Zhao, Jing Lin, Nan Jiang, Qiang Xu, Cheng-Cheng Zhu, Jain-Qiu Qu, Lin Cong, Hui Li
Journal: International journal of ophthalmology (2014): 441–447

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Qing Li, Jiang Lu, Xianyu Wang
Journal: Neural regeneration research (2014): 2002

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Nan Liang, Peng Wang, Shilei Wang, Shuhong Li, Yu Li, Jinying Wang, Min Wang
Journal: Journal of Neural Transmission (2014): 593–600

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: M Hagiyama, T Inoue, T Furuno, T Iino, S Itami, M Nakanishi, H Asada, Y Hosokawa, A Ito
Journal: British Journal of Dermatology (2013): 771–778

相关产品