钙离子荧光探针Oregon Green 488 BAPTA-1,AM 货号20507-AAT Bioquest荧光染料

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钙离子荧光探针Oregon Green 488 BAPTA-1,AM

钙离子荧光探针Oregon Green 488 BAPTA-1,AM

钙离子荧光探针Oregon Green 488 BAPTA-1,AM    货号20507 货号 20507 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 2544
Ex (nm) 493 Em (nm) 522
分子量 1258.07 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:20507

产品名称:钙离子荧光探针Oregon Green 488 BAPTA-1,AM

规格:10×50 ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1258.07

溶剂:DMSO

激发波长(nm):494

发射波长(nm):523

 

适用仪器


流式细胞仪  
激发: 488nm激光
发射: 530/30nm滤波片
通道: FITC通道
荧光显微镜  
激发: FITC
发射: FITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 490nm
发射: 525nm
cutoff: 515nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

OG488 BAPTA -1 AM 与 Oregon Green 488 BAPTA-1 AM 酯的分子相同。它是一种细胞渗透性和可见光可激发的钙指示剂,通常与 FITC 滤光片组一起使用。通过将溶解的指示剂直接添加到含有培养细胞的培养皿中,可以为细胞加载 OG488 BAPTA -1 AM。来自这些细胞的荧光信号通常使用荧光显微镜、荧光微孔板测定或流式细胞术测量。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Oregon Green 488 BAPTA-1,AM。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61

A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Authors: Tada M, Takeuchi A, Hashizume M, Kitamura K, Kano M.
Journal: Eur J Neurosci (2014): 1720

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339

Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, Fandrey J.
Journal: Am J Physiol Cell Physiol (2011): C266

Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804

说明书
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