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Xite Red β-D-吡喃半乳糖苷*红色荧光*
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货号 | 14035 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mg | 价格 | 2412 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 591.63 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
Xite Red β-D-半乳糖吡喃糖苷是β-半乳糖苷酶（β-gal）的荧光底物。与现有的β-半乳糖苷酶底物（例如，常用的FDG）相比，它具有更好的细胞通透性。Xite Red β-D-吡喃半乳糖苷很容易进入细胞，并被β-gal裂解，从而产生强荧光产品Xite Red。强烈荧光的Xite Red可以很好地保留在细胞中，从而易于通过流式细胞仪和荧光显微镜进行检测。Xite Red β-D-半乳糖吡喃糖苷提供了一种简单而灵敏的工具来检测β-半乳糖苷酶的活性。Xite Red β-D-吡喃半乳糖苷可以作为检测细胞中细胞衰老的工具，因为β-gal已被确定为细胞衰老的可靠标记。此外，Xite Red β-D-吡喃半乳糖苷是可固定的。Xite Red β-D-吡喃半乳糖苷产生的红色荧光可以很容易地与其他颜色的荧光探针（例如DAPI或GFP）结合使用，以进行多色荧光分析。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Xite Red β-D-半乳糖吡喃糖苷。
适用仪器
| 流式细胞仪 | |
| 激发： | 488nm激光 |
| 发射： | 575/26 nm滤波片 |
| 通道： | PE滤波片组 |
| 荧光显微镜 | |
| 激发： | Cy3/TRITC滤波片 |
| 发射： | Cy3/TRITC滤波片 |
| 推荐孔板： | 黑色透明 |
产品说明书
样品实验方案
以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。
简要概述
- 根据需要处理样品
- 准备Xite Red β-D-吡喃半乳糖苷工作溶液并将其添加到样品中
- 在37°C下孵育样品15至45分钟
- 使用带有575/26 nm滤光片的流式细胞仪（PE通道）或带有Cy3/TRITC滤光片组的荧光显微镜检测荧光强度
溶液配制
储备溶液配制
操作步骤
- 根据需要处理样品。
- 处理并用自备的缓冲液（例如DPBS）洗涤细胞。
- 加入Xite Red β-D-吡喃半乳糖苷工作溶液15-45分钟，然后在37°C的培养箱中培养样品。
注意：孵育的最佳时间需要通过实验确定。 - 取出工作溶液并用自备的缓冲液洗涤细胞。
- 将细胞重悬在自备的缓冲液中，并使用流式细胞仪使用575/26 nm滤光片（PE通道）或带有Cy3/TRITC滤光片组的荧光显微镜检测荧光强度。
图示
 图1.用Xite Red β-D-吡喃半乳糖苷测量β-gal的表达。将9L-LacZ细胞（过度表达β-gal的细胞）与Xite Red β-D-吡喃半乳糖苷在37°C下孵育30分钟。使用NovoCyte流式细胞仪（ACEA Biosciences）通过PE通道获取信号。 |
参考文献
Acridinium Benzoates for Ratiometric Fluorescence Imaging.
Authors: Wen, Min and Wang, Xijing and Wang, Ting and Sun, Yan and Fan, Mengting and Li, Min and Zhu, Junru and Zhang, Dazhi and Cui, Xiaoyan and Shan, Yongkui
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 3247-3251
Fluorescence Signal Amplification by Using β-Galactosidase for Flow Cytometry; Advantages of an Endogenous Activity-Free Enzyme.
Authors: Nobori, Takanobu and Kawamura, Masumi and Yoshida, Ryosuke and Joichi, Taisei and Kamino, Kenta and Kishimura, Akihiro and Baba, Eishi and Mori, Takeshi and Katayama, Yoshiki
Journal: Analytical chemistry (2020): 3069-3076
Amphiphilic triphenylamine-benzothiadiazole dyes: preparation, fluorescence and aggregation behavior, and enzyme fluorescence detection.
Authors: Ishi-I, Tsutomu and Kawai, Kazuki and Shirai, Yuya and Kitahara, Ikumi and Hagiwara, Yoshinori
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (2019): 1447-1460
Macrotheranostic Probe with Disease-Activated Near-Infrared Fluorescence, Photoacoustic, and Photothermal Signals for Imaging-Guided Therapy.
Authors: Zhen, Xu and Zhang, Jianjian and Huang, Jiaguo and Xie, Chen and Miao, Qingqing and Pu, Kanyi
Journal: Angewandte Chemie (International ed. in English) (2018): 7804-7808
Impact of plasma protein binding on cargo release by thermosensitive liposomes probed by fluorescence correlation spectroscopy.
Authors: Mittag, Judith J and Kneidl, Barbara and Preiβ, Tobias and Hossann, Martin and Winter, Gerhard and Wuttke, Stefan and Engelke, Hanna and Rädler, Joachim O
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V (2017): 215-223
Isolation and Fluorescence-Activated Cell Sorting of Mouse Keratinocytes Expressing β-Galactosidase.
Authors: Kasper, Maria and Toftgård, Rune and Jaks, Viljar
Journal: Methods in molecular biology (Clifton, N.J.) (2016): 123-36
Small quinolinium-based enzymatic probes via blue-to-red ratiometric fluorescence.
Authors: Wang, Pan and Du, Jiajun and Liu, Huijing and Bi, Guoqiang and Zhang, Guoqing
Journal: The Analyst (2016): 1483-7
Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo.
Authors: Asanuma, Daisuke and Sakabe, Masayo and Kamiya, Mako and Yamamoto, Kyoko and Hiratake, Jun and Ogawa, Mikako and Kosaka, Nobuyuki and Choyke, Peter L and Nagano, Tetsuo and Kobayashi, Hisataka and Urano, Yasuteru
Journal: Nature communications (2015): 6463
A fluorescence-based method coupled with Disruptor filtration for rapid detection of F + RNA phages.
Authors: Yang, Y and Griffiths, M W
Journal: Letters in applied microbiology (2014): 177-83
Quantitative Fluorescence Assays Using a Self-Powered Paper-Based Microfluidic Device and a Camera-Equipped Cellular Phone.
Authors: Thom, Nicole K and Lewis, Gregory G and Yeung, Kimy and Phillips, Scott T
Journal: RSC advances (2014): 1334-1340