Cell Meter 细胞凋亡活性检测试剂盒*红色荧光*

	货号	23007	存储条件	Multiple
	规格	100 Tests	价格	4272
	Ex (nm)	544	Em (nm)	567
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：23007

产品名称：Cell Meter 细胞凋亡活性检测试剂盒

规格：100 Tests

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：N/A

溶剂：DMSO

激发波长(nm)：N/A

发射波长(nm)：N/A

产品介绍

细胞衰老是触发不可逆的生长停滞，以防止DNA损伤的细胞生长。衰老相关的β-半乳糖苷酶在衰老细胞中高度过表达，已被广泛用作衰老标记。比色X-gal染色法广泛用于检测衰老细胞中的SA-beta-gal。但是，着色方法有一些局限性，例如需要固定细胞（由于X-gal的细胞通透性低），染色时间较长和灵敏度低。Cell Meter 细胞凋亡活性检测试剂盒使用红色β-D-吡喃半乳糖苷，一种荧光性β-Gal底物，易于进入活细胞，并被细胞内的β-半乳糖苷酶裂解，产生强烈的红色荧光。与不渗透细胞的X-Gal底物不同，它具有出色的细胞渗透性。强大的Cell Meter 细胞凋亡活性检测试剂盒使用户能够以更高的灵敏度检测衰老。Xite Redβ-D-吡喃半乳糖苷也可以固定用于进一步的细胞分析。Xite Red的红色荧光可以很容易地与其他彩色荧光探针（例如DAPI或GFP）结合使用。Xite Red产品可以很好地保留在细胞内部，为荧光成像和流式细胞仪分析产生稳定的信号。

点击查看光谱

图示

图1.使用NovoCyte流式细胞仪（ACEA Biosciences），用Cell Meter 细胞凋亡活性检测试剂盒进行固定检测。将9L-LacZ细胞与DMSO或Xite Redβ-D-吡喃半乳糖苷在37°C下孵育45分钟。使用PE通道获取固定之前和之后的信号。（然后将细胞在室温下用4％甲醛固定20分钟，然后清洗一次。）

图2. 9L-LacZ细胞的荧光图像。将9L-LacZ细胞直接用Xite Redβ-D-吡喃半乳糖苷在37°C的细胞培养基中染色45分钟。使用Cy3 / TRITC滤波器组收集信号。

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