鬼笔环肽-PEG4-DBCO 货号5303-AAT Bioquest荧光染料

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鬼笔环肽-PEG4-DBCO

鬼笔环肽-PEG4-DBCO

鬼笔环肽-PEG4-DBCO    货号5303 货号 5303 存储条件 在零下15度以下保存, 避免光照
规格 100 ug 价格 3648
Ex (nm) Em (nm)
分子量 1366.55 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:5303

产品名称:鬼笔环肽-PEG4-DBCO

规格:100 ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1366.55

外观:固体

溶剂:DMSO

 

产品介绍

鬼笔环肽-PEG4-DBCO是美国AAT Bioquest生产的鬼笔环肽缀合物,鬼笔环肽Phalloidin标记探针会选择性地结合F-肌动蛋白。以纳摩尔浓度使用,鬼笔环肽衍生物是方便的探针,用于标记,鉴定和定量甲醛固定和透化组织切片中的F-肌动蛋白,细胞培养物或无细胞实验。肌动蛋白是一种球状的,大约42kDa的蛋白质,几乎存在于所有真核细胞中。 它也是最高度保守的蛋白质之一,与藻类和人类不同的物种相差不超过20%。 肌动蛋白是细胞中两种细丝的单体亚基:微丝,细胞骨架的三个主要组分之一,以及微丝,是肌细胞中收缩装置的一部分。 因此,肌动蛋白参与许多重要的细胞过程,包括肌肉收缩,细胞运动,细胞分裂和胞质分裂,囊泡和细胞器运动,细胞信号传导,以及细胞连接和细胞形状的建立和维持。

鬼笔环肽比肌动蛋白单体更紧密地结合肌动蛋白丝,导致肌动蛋白亚基从丝状末端解离的速率常数降低,基本上通过防止丝解聚来稳定肌动蛋白丝。此外,发现鬼笔环肽抑制F-肌动蛋白的ATP水解活性。鬼笔环肽在细胞中以不同浓度起作用不同。当以低浓度引入细胞质时,鬼笔环肽将较少聚合形式的细胞质肌动蛋白以及微丝蛋白聚集成聚集的肌动蛋白聚合物的稳定“岛”,但它不会干扰应力纤维,即厚的微丝束。鬼笔环肽的性质是通过用荧光类似物标记鬼笔环肽并用它们染色肌动蛋白丝用于光学显微镜来研究F-肌动蛋白在细胞中的分布的有用工具。鬼笔环肽的荧光衍生物已经证明在定位活细胞或固定细胞中的肌动蛋白丝以及在体外可视化单个肌动蛋白丝方面非常有用。荧光鬼笔环肽衍生物已被用作高分辨率肌动蛋白研究中的重要工具。 AAT Bioquest可以提供多色成像应用的鬼笔环肽。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的鬼笔环肽-PEG4-DBCO。 

 

参考文献

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Cell-Permeable, MMP-2 Activatable, Nickel Ferrite and His-tagged Fusion Protein Self-Assembled Fluorescent Nanoprobe for Tumor Magnetic Targeting and Imaging
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DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
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Enhanced bovine serum albumin absorption on the N-hydroxysuccinimide activated graphene oxide and its corresponding cell affinity
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Enhanced osteointegration of tantalum-modified titanium implants with micro/nano-topography
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Journal: RSC Advances (2017): 46472–46479

Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs
Authors: Wang, Dan and Liu, Mengya and Gu, Shuangying and Zhou, Yue and Li, Song
Journal: Journal of Biomaterials and Nanobiotechnology (2017): 189–201

Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery
Authors: Chang, Ting-Ya and Chen, Chen and Lee, Min and Chang, Ya-Chu and Lu, Chi-Huan and Lu, Shao-Tzu and Wang, De-Yao and Wang, Aijun and Guo, Chin-Lin and Cheng, Pei-Lin
Journal: eLife (2017): e31101

Study on the Regulation of Focal Adesions and Cortical Actin by Matrix Nanotopography in 3D Environment
Authors: Han, Jingjing and Lin, Keng-hui and Chew, Lock Yue
Journal: Journal of Physics: Condensed Matter (2017)

The correlation between osteopontin adsorption and cell adhesion to mixed self-assembled monolayers of varying charges and wettability
Authors: Hao, Lijing and Li, Tianjie and Yang, Fan and Zhao, Naru and Cui, Fuzhai and Shi, Xuetao and Du, Chang and Wang, Yingjun
Journal: Biomaterials Science (2017)

Cell Morphology Modified by Biomaterial Induces miR-21 Expression and Apoptosis
Authors: Liu, Mengya and Gu, Shuangying and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2016): 42

说明书
鬼笔环肽-PEG4-DBCO.pdf