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–Bacstain– CTC Rapid Staining Kit (for Microscopy)
–Bacstain– CTC Rapid Staining Kit (for Microscopy)
- 細菌研究用試薬
菌染色キット
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製品コードBS02 –Bacstain– CTC Rapid Staining Kit (for Microscopy)
| 容 量 | メーカー希望 小売価格 |
富士フイルム 和光純薬 |
|---|---|---|
| 100 assays | ￥22,800 | 345-91311 |
| 100 assays | ・CTC ・Enhancing Reagent B |
10 mg × 3 500 μl × 1 |
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マニュアル
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取扱説明書
日本語 
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取扱説明書 English
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プロトコル 細菌を蛍光染色したい
技術情報
キット以外に必要なもの
・20 μL, 1000 μLマイクロピペット ・インキュベーター
・蛍光顕微鏡（blue excitation filter, red emission filter)
参考文献
1) A. W. Coleman, "Enhanced Detection of Bacteria in Natural Environments by Fluorochrome Staining of DNA", Limnol. Oceanogr., 1980, 25, 948.
2) E. Severin, J. Stellmach and H. -M. Nachtigal, "Fluorimetric Assay of Redox Activity in Cells", Anal. Chim. Acta, 1985, 170, 341.
3) G. G. Rodriguez, D. Phipps, K. Ishiguro and H. F. Ridgway, "Use of a Fluorescent Redox Probe for Direct Visualization of Actively Respiring Bacteria", Appl. Environ. Microbiol., 1992, 58(6), 1801.
4) G. Schaule, H. C. Flemming and H. F. Ridgway, "Use of 5-Cyano-2,3-ditolyl Tetrazolium Chloride for Quantifying Planktonic and Sessile Respiring Bacteria in Drinking Water", Appl. Environ. Microbiol., 1993, 59(11), 3850.
5) R. A. Bovill, J. A. Shalloross and B. M. Markey, "Comparison of the Fluorescent Redox Dye 5-Cyano-2,3-ditolyltetrazolium Chloride with p-Iodonitrotetrazolium Violet to Detect Metabolic Activity in Heat-stressed Listeria monocytogenes Cells", J. Appl. Bacteriol., 1994, 77(4), 353.
6) M. T. E. Suller and D. Lloyd, "Flow Cytometric Assesment of the Postantibiotic Effect of Methicillin on Staphylococcus aureus", Antimicrob. Agents Chemother., 1998, 42(5), 1195.
7) M. Kawai, N. Yamaguchi and M. Nasu "Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process", J. Appl. Microbiol., 1999, 86, 496.
8) N. Yamaguchi, M. Sasada, M. Yamanaka and M. Nasu, "Rapid Detection of Respiring Escherichia coli O157:H7 in Apple Juice, Milk and Ground Beef by Flow Cytometry", Cytometry, 2003, 54A, 27.
9) A. Kitaguchi, N. Yamagauchi and M. Nasu, "Enumeration of Respiring Pseudomonas spp. in Milk within 6 Hours by Fluorescence In Situ Hybridization Following Formazan Reduction", Appl. Environ. Microbiol., 2005, 71(5), 2748.
10)A. Hiraishi and N. Yoshida, "An Improved Redox Dye-Staining Method Using 5-Cyano-2,3-Ditoryl Tetrazolium Chloride for Detection of Metabolically Active Bacteria in Activated Sludge", Microbes Environ., 2004, 19(1), 61.
11)平石 明，吉田 奈央子，“活性汚泥における培養不能な細菌の検出”，月刊 海洋 培養不能細菌 -VNC研究の現状と課題-，2003, 33, 48．
12)染谷 孝， “蛍光染色による土壌微生物の検出法”， 月刊 海洋 培養不能細菌 -VNC研究の現状と課題-， 2003, 33, 14．
取扱条件
| 1.医薬用外劇物, 2.保存方法：冷蔵,遮光 | |
| 法規制毒物および劇物取締法 | 劇物 |
|---|---|
