PROTEOSTAT® PDI assay kit 蛋白二硫键异构酶检测试剂盒

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PROTEOSTAT® PDI assay kitPROTEOSTAT®  PDI assay kit                              蛋白二硫键异构酶检测试剂盒

蛋白二硫键异构酶检测试剂盒

PROTEOSTAT® PDI 检测试剂盒能够以稳定且高通量的方式定量检测PDI酶活性,适用于从化学库中筛选PDI抑制剂,为筛选PDI酶活性调节剂提供了一种简单、均相的检测方法。

本试剂盒在二硫苏糖醇(DTT)存在的条件下,PDI酶催化胰岛素聚集体的形成,随后与发射红色荧光的 PROTEOSTAT® PDI检测染料结合,通过监测胰岛素减少来实现对PDI酶活性的检测。

相对于使用比浊法检测PDI酶活性,基于荧光的检测法有着极大改进的信号检测窗口和检测下限,以及优越的 Z' 因子(>0.8)。检测中板内和板间CV值为3-5%。

本试剂盒中包含人重组PDI酶(EC 5.3.4.1)、杆菌肽(PDI抑制剂)以及监测PDI酶活性变化所需的全部试剂。

◆产品详情


基础信息


别称:蛋白二硫键异构酶(Protein disulfide isomerase)检测试剂盒

应用:荧光检测

储存条件:避光。避免反复冻融。

运输方式:干冰运输

长期储存温度:–80°C

特点:本试剂盒为筛选蛋白二硫键异构酶(PDI)酶活性的调节剂提供了一种简单、均相的检测方法。

UniProt ID:P07237(人)

监管状态:RUO – 仅供研究使用

技术信息/产品说明:Enzo和PROTEOSTAT®是Enzo Life Sciences, Inc. 的商标。

技术信息/产品说明:Enzo的部分产品和产品应用受美国、外国专利及正在申请的专利保护。



品质保证


(1)按照手册中的步骤, PROTEOSTAT® PDI检测试剂盒可用于检测以下样品:

(1)① 含PDI的胰岛素;② 不含PDI的胰岛素;③ 含有PDI和杆菌肽的胰岛素。

(2)Z' 因子高于0.8。

(3)PDI活性至少可被杆菌肽抑制50%。



使用/稳定性


在产品标签注明的日期前,试剂盒组分在适当的储存条件下可稳定保存。

可存放于-20°C的无霜冰箱中,或在–80°C下长期保存。



组分


● PROTEOSTAT® PDI 检测试剂

● PDI(人)(重组)

● 胰岛素(冻干)

● 杆菌肽(抑制剂对照,冻干)

● PBE 缓冲液

● 终止试剂

● DTT(10 mM)

● 去离子水


※ 本页面产品仅供研究用,研究以外不可使用。

参考文献


 1. 

Thioredoxin reductase is a major regulator of metabolism in leukemia cells: S. Karunanithi, et al.; Oncogene 41388 (2021), Abstract;


 2.

Molecular docking‐assisted screening reveals tannic acid as a natural protein disulphide isomerase inhibitor with antiplatelet and antithrombotic activities: L. Ren, et al.; J. Cell. Mol. Med. 24, 14257 (2020), AbstractFull Text

 3.

PDI knockdown inhibits seizure activity in acute seizure and chronic epilepsy rat models via S-nitrosylation-independent thiolation on NMDA receptor: A.R. Jeon, et al.; Front. Cell. Neurosci. 12, 438 (2018), Application(s): Hippocampal tissue lysates, AbstractFull Text

 4.

The thiosemicarbazone Me2NNMe2 induces paraptosis by disrupting the ER thiol redox homeostasis based on protein disulfide isomerase inhibition: S. Hager, et al.; Cell Death Dis. 9, 1052 (2018), AbstractFull Text

 5.

Tissue Factor Coagulant Activity is Regulated by the Plasma Membrane Microenvironment: Y. Yu, et al.; Thromb. Haemost. 118, 990 (2018), Application(s): In-vitro activity assay, Abstract;


 6.

Curcumin and Curcuma longa L. extract ameliorate lipid accumulation through the regulation of the endoplasmic reticulum redox and ER stress: H.Y. Lee, et al.; Sci. Rep. 7, 6513 (2017), Application(s): PDI assay of tissue lysates, AbstractFull Text

 7.

Nox4 regulates the eNOS uncoupling process in aging endothelial cells: H.Y. Lee, et al.; Free Radic. Biol. Med. 113, 26 (2017), Application(s): PDI assay of HUVEC cell lysates, Abstract;


 8.

PDI regulates seizure activity via NMDA receptor redox in rats: J.Y. Kim, et al.; Sci. Rep. 7, 42491 (2017), Application(s): Hippocampal tissue lysates, Abstract; Full Text

 9.

Protein disulfide isomerase regulates renal AT1 receptor function and blood pressure in rats: X. Wang, et al.; Am. J. Physiol. Renal. Physiol. 313, F461 (2017), Application(s): Renal tissue lysates, AbstractFull Text

10.

Requirement of zinc transporter SLC39A7/ZIP7 for dermal development to fine-tune endoplasmic reticulum function by regulating protein disulfide isomerase: B.H. Bin, et al.; J. Invest. Dermatol. 137, 1682 (2017), Application(s): ER fractions from murine osteoblasts, Abstract;


11. 

Succination of protein disulfide isomerase links mitochondrial stress and endoplasmic reticulum stress in the adipocyte during diabetes: A.M. Manuel, et al.; Antioxid. Redox Signal. 27, 1281 (2017), Application(s): 3T3-L1 adipocytes and adipose tissue lysates, AbstractFull Text

12.

Adverse outcomes associated with cigarette smoke radicals related to damage to protein disulfide isomerase: H. Kenche, et al.; J. Biol. Chem. 291, 4763 (2016), Abstract;


13.

Adverse Outcomes Associated with Cigarette Smoke Radicals Related to Damage to Protein-disulfide Isomerase: H. Kenche, et al.; J. Biol. Chem. 291, 4763 (2016), Application(s): in-vitro assay with recombinant PDI, AbstractFull Text

14.

Role of two sequence motifs of mesencephalic astrocyte-derived neurotrophic factor in its survival-promoting activity: K. Mätlik, et al.; Cell Death Dis. 6, e2032 (2015), AbstractFull Text

15.

Methods of measuring protein disulfide isomerase activity: a critical overview: M.M. Watanabe, et al.; Front. Chem. 2, 73 (2014), Application(s): Review on available assays to measure PDI activity, AbstractFull Text

16.

N-octanoyl dopamine treatment of endothelial cells induces the unfolded protein response and results in hypometabolism and tolerance to hypothermia: E. Stamellou, et al.; PLoS One 9, e99298 (2014), AbstractFull Text

17.

The protein disulfide isomerases PDIA4 and PDIA6 mediate resistance to cisplatin-induced cell death in lung adenocarcinoma: G. Tufo, et al.; Cell Death Differ. 21, 685 (2014), Application(s): Evaluation of PDI activity in whole extracts and ER fractions, Abstract;


18.

Rapid activation of monocyte tissue factor by antithymocyte globulin is dependent on complement and protein disulfide isomerase: F. Langer, et al.; Blood 121, 2324 (2013), Abstract;


19.

Oxidative processing of latent Fas in the endoplasmic reticulum controls the strength of apoptosis: V. Anathy, et al.; Mol. Cell. Biol. 32, 3464 (2012), Abstract;



◆一般参考文献


 1. 

Attachment and entry of Chlamydia have distinct requirements for host protein disulfide isomerase: S. Abromaitis & R.S. Stephens; PLoS Pathog. 5, e1000357 (2009), Abstract;


 2.

Protein disulfide isomerase (PDI) associates with NADPH oxidase and is required for phagocytosis of Leishmania chagasi promastigotes by macrophages: C. X. Santos, et al.; J. Leukoc. Biol. 86, 989 (2009), Abstract;


 3.

The proteostasis boundary in misfolding diseases of membrane traffic: D.M. Hutt, et al.; FEBS Lett. 583, 2639 (2009), Abstract;


 4.

Increasing melanoma cell death using inhibitors of protein disulfide isomerases to abrogate survival responses to endoplasmic reticulum stress: P.E. Lovat, et al.; Cancer Res. 68, 5363 (2008), Abstract;


 5.

Targeting homeostatic mechanisms of endoplasmic reticulum stress to increase susceptibility of cancer cells to fenretinide-induced apoptosis: the role of stress proteins ERdj5 and ERp57: M. Corazzari, et al.; Br. J. Cancer 96, 1062 (2007), Abstract;


 6.

Coupling endoplasmic reticulum stress to the cell death program: R.V. Rao, et al.; Cell Death Differ. 11, 372 (2004), Abstract;


 7.

Protein-disulfide isomerase-mediated reduction of two disulfide bonds of HIV envelope glycoprotein 120 occurs post-CXCR4 binding and is required for fusion: R. Barbouche, et al.; J. Biol. Chem. 278, 3131 (2003), Abstract;


 8.

Protein disulfide isomerase, a component of the estrogen receptor complex, is associated with Chlamydia trachomatis serovar E attached to human endometrial epithelial cells: C.H. Davis, et al.; Infect. Immun. 70, 3413 (2002), Abstract;


 9.

Protein-disulfide isomerase-mediated reduction of the A subunit of cholera toxin in a human intestinal cell line: P.A. Orlandi; J. Biol. Chem. 272, 4591 (1997), Abstract;


10.

Acceleration of reactivation of reduced bovine pancreatic ribonuclease by a microsomal system from rat liver: R.F. Goldberger, et al.; J. Biol. Chem. 238, 628 (1963), Abstract;


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