ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X* 货号17300-AAT Bioquest荧光染料

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ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X*

ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X*

货号 17300 存储条件 在零下15度以下保存, 避免光照
规格 500 Tests 价格 1140
Ex (nm) Em (nm)
分子量 N/A 溶剂



ReadiView Blue 实时荧光定量 PCR 可视化染料 是一种 200X 浓缩的即用型惰性染料,可增强实时 PCR 反应的可见性,从而实现更准确的移液和板装载。当按照指示使用时,染料的存在不会对 PCR 反应的性能产生不利影响,并且不会干扰 Helixyte Green-、SYBR® Green- 或其他探针的检测。它针对各种实时 PCR 混合物和试剂盒进行了优化,包括含有 ROX 的混合物以及用于 FAST 和常规反应时间的混合物。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiView Blue 实时荧光定量 PCR 可视化染料 *200X*。 




以下方案仅作为指导,应根据您的具体应用进行优化。开始实验前,ReadiView Blue 实时荧光定量 PCR 可视化染料必须完全解冻至室温。


1.1 将 1 µL ReadiView Blue 实时荧光定量 PCR 可视化染料与包含引物、TaqMan 探针或 DNA 染料的 100 µL TAQuest qPCR Master Mix(2X master mix)混合。

1.2 彻底混合各组分,然后短暂离心以将溶液收集在管底。 储存在冰上,避光。

1.3对于 20 μL PCR 反应,将 10 μL 的 qPCR 主混合物与 10 μL 的 DNA 样本混合。 建议放在白色背景上以提高可见度。

注意ReadiView Blue 实时荧光定量 PCR 可视化染料也可能与各种预混液兼容,但应由用户验证以获得最佳性能。


2.在 DNA 或 RNA 样品中添加染料:

2.1 将 1 µL ReadiView Blue 实时荧光定量 PCR 可视化染料与100 µL 含有 DNA 或 RNA 的样品混合

2.2 彻底混合各组分,然后短暂离心以将溶液收集在管底。 储存在冰上,避光。

2.3 对于 20 μL PCR 反应,将 10 μL 的 qPCR 主混合物与 10 μL 的 DNA 或 RNA 样品混合。 建议放在白色背景上以提高可见度。



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[Typing of Leishmania Species Causing Cutaneous Leishmaniasis by Sybr Green Based ITS-1 Real Time Polymerase Chain Reaction Method].
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Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites.
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Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.
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Multiplex SYBR Green real-time PCR for Lactobacillus acidophilus group species targeting biomarker genes revealed by a pangenome approach.
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Authors: Cong, Yulin and Sun, Yixue and Deng, Xiaoyu and Yu, Haiying and Lian, Xiaohuan and Cong, Yanlong
Journal: Journal of virological methods (2022): 114387
One-step SYBR green-based real-time RT-PCR assay for detection of foot-and-mouth disease virus circulating in India.
Authors: Biswal, Jitendra K and Jena, Biswa Ranjan and Ali, Syed Zeeshan and Ranjan, Rajeev and Mohapatra, Jajati K and Singh, Rabindra Prasad
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Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis.
Authors: Sarkar, Shovon Lal and Alam, A S M Rubayet Ul and Das, Prosanto Kumar and Pramanik, Md Hasan Ali and Al-Emran, Hassan M and Jahid, Iqbal Kabir and Hossain, M Anwar
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Journal: Molecular and cellular probes (2021): 101730

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