Gelite 红色核酸凝胶染色 100X水溶液 (停产) 货号17603-AAT Bioquest荧光染料

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Gelite 红色核酸凝胶染色 100X水溶液 (停产)

Gelite 红色核酸凝胶染色 100X水溶液 (停产)

Gelite 红色核酸凝胶染色 100X水溶液 (停产)    货号17603 货号 17603 存储条件 在零下15度以下保存, 避免光照
规格 10 mL 价格 0
Ex (nm) 538 Em (nm) 609
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:17603

产品名称:Gelite 红色核酸凝胶染色剂 100X 水溶液

规格:10ml

储存条件:保存在冰箱-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

外观:液体

激发波长(nm):538

发射波长(nm):609

 

产品介绍

Gelite 红色核酸凝胶染色剂 100X 水溶液 是美国AAT Bioquest生产的核酸染料,Gelite Red是我们Gelite 核酸凝胶染色家族的最新成员。现在,我们拥有一整套多色凝胶染色剂,用于检测凝胶中的核酸。Gelite Red是一种极其敏感的核酸凝胶染色剂,用于使用标准的300 nm UV透射仪和Polaroid 667黑白打印胶片检测凝胶中的DNA。在相同条件下,它比流行的SYBR Gold凝胶染色剂更敏感。这种显着的敏感性可归因于Gelite Red的独特染料特性的组合。因为核酸结合的Gelite Red染料显示出接近488nm和~300nm的激发最大值(发射最大值为~610nm),与各种仪器兼容,包括紫外线外延和透射仪和蓝光透射仪,以及基于汞弧灯和氩离子激光的凝胶扫描仪。我们的Gelite Red是SYBR Gold,SYBR Safe和Ethidium Bromide的优质替代品。它为染色凝胶中的核酸样品提供了方便的解决方案。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Gelite 红色核酸凝胶染色剂。 

 

图示

Gelite 红色核酸凝胶染色 100X水溶液 (停产)    货号17603

凝胶染色后Gelite Red和SYBR Gold的比较。 将1kb Plus DNA Ladder(每泳道200ng和100ng)在1×TAE缓冲液中的0.9%琼脂糖中进行电泳。 随后将凝胶在1x TAE中用1x Gelite Red或SYBR Gold染色30分钟,并使用具有EtBr滤光剂的ChemiDoc MP Imager拍照。

 

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参考文献

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Journal: IUBMB Life (2005): 745-7

Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products
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An enzymatic procedure for the purification of DNA restriction fragments without gel electrophoresis and ethidium bromide staining
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Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)
Authors: V. L. Singer
Journal: Mutat Res (1999): 37-47

Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis
Authors: I. Y. Belyaev
Journal: Biochim Biophys Acta (1999): 348-56

Genotyping microsatellite polymorphisms by agarose gel electrophoresis with ethidium bromide staining: application to quantitative trait loci analysis of seizure susceptibility in mice
Authors: T. N. Ferraro
Journal: Psychiatr Genet (1998): 227-33

The effect of ethidium bromide on mobility of DNA fragments in agarose gel electrophoresis
Authors: J. Sigmon
Journal: Electrophoresis (1996): 1524-7

Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus
Authors: S. F. Zakharov
Journal: Anal Biochem (1995): 195-8

Ethidium bromide staining during denaturation with glyoxal for sensitive detection of RNA in agarose gel electrophoresis
Authors: D. Grundemann
Journal: Anal Biochem (1994): 459-61

High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange
Authors: H. S. Rye
Journal: Nucleic Acids Res (1991): 327-33

说明书
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