LBIS® 血蓝蛋白(KLH)(T细胞依赖性抗原) 大鼠免疫球蛋白M(IgM) ELISA试剂盒 LBIS® KLH(TDAR) Rat-IgM ELISA Kit

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LBIS® 血蓝蛋白(KLH)(T细胞依赖性抗原)  大鼠免疫球蛋白M(IgM) ELISA试剂盒                              LBIS® KLH(TDAR) Rat-IgM ELISA KitLBIS® 血蓝蛋白(KLH)(T细胞依赖性抗原)

大鼠免疫球蛋白M(IgM) ELISA试剂盒

 


  药品的免疫毒性实验相关方针ICH S8中推荐在无特定免疫毒性标靶情况下进行的T细胞依赖性抗体产生实验(TDAR、T cell Dependent Antibody Reaction)。TDAR被称为T细胞依赖性抗原。例如:通过KLH(Keyhole limpet hemocyanin)的投放对一次抗原刺激而生成IgM性状抗体。接下来进行二次抗原刺激后通过类别转换作用可观察到产生IgG性状抗体。由于本试剂盒可简便测定大鼠血液中IgM性状的抗KLH浓度,所以十分符合上文所述的目的。请与大鼠抗KLH-IgG检测试剂盒配套使用。

◆特点

LBIS® 血蓝蛋白(KLH)(T细胞依赖性抗原)  大鼠免疫球蛋白M(IgM) ELISA试剂盒                              LBIS® KLH(TDAR) Rat-IgM ELISA Kit


● 测定时间短(总反应时间:2小时20分钟)。

● 微量样本即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

 

◆试剂盒组成


组成品

状态

包装

KLH包被96孔板

清洗后使用

96 wells(8×12)/1个

抗KLH大鼠IgM标准溶液(1000 ng/mL)

稀释后使用

200 μL/1瓶

缓冲液

直接使用

100 mL/1瓶

HRP结合抗大鼠IgM抗体

稀释后使用

100 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4)※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


◆物种交叉性


2000 ng/mL时数据—:无交叉性


动物种类

对象物质

反应性及反应率(%)

大鼠

IgM

100

IgG

IgA

IgE

小鼠

IgG

IgM

IgE

 


◆样本信息


● 大鼠血清·血浆

● 50 μL/well(稀释样本)

※ 用附带的缓冲液将样本稀释至标准曲线范围内。

※ 为了避免非特异反应发生,请将样本稀释200倍以上。

 


测定范围


3.13~200 ng/mL

(标准曲线范围)

 


◆实验数据


精密度实验(实验内变化)


样本

A

B

1

49.5

164

2

47.7

166

3

48.0

164

4

47.6

161

5

49.7

169

Mean

48.5

165

SD

1.03

2.93

CV(%)

2.1

1.8

单位:ng/mL



重复性实验(实验间变化)


测定日/样本

E

F

G

0天

11.1

86.5

187

1天

11.1

86.1

188

2天

11.2

86.3

188

3天

11.1

83.3

186

Mean

11.1

85.6

187

SD

0.0576

1.48

0.814

CV(%)

0.52

1.7

0.44

单位:ng/mL,n=4



添加回收实验


样本C


添加量

实测值

回收量

回收率(%)

0.00

47.3

102

144

96.7

94.8

127

172

125

98.4

140

182

135

96.4

单位:ng/mL,n=2


样本D


添加量

实测值

回收量

回收率(%)

0.00

34.8

16.8

51.9

17.1

102

25.2

59.5

24.7

98.0

42.0

76.7

41.9

99.8

单位:ng/mL,n=2



稀释直线性实验


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.9965~0.9998

产品编号 产品名称 产品规格 产品等级
633-13769 LBIS® KLH(TDAR) Rat-IgM ELISA Kit
LBIS® 血蓝蛋白(KLH)(T细胞依赖性抗原)大鼠免疫球蛋白M(IgM) ELISA试剂盒
96 tests

LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒 LBIS® OVA-IgG1 Mouse

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LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒                              LBIS® OVA-IgG1 MouseLBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒

 


  IgG是血清中免疫球蛋白中含量最高的二次免疫应答主要抗体。占据三分之二IgG的是具有γ1的H链,分子量146 kDa的IgG1。以OVA(卵清蛋白)作为特殊化抗原,通过简化测定抗OVA- IgG1抗体值来阐明小鼠免疫系统作用机理。

  小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒是仅特异性测定Anit-OVA-IgG1的试剂盒。

 

◆特点

LBIS® 小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1)ELISA试剂盒                              LBIS® OVA-IgG1 Mouse


● 测定时间短(总反应时间:1小时50分钟)

● 微量样本即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

● 操作简便,无特殊前处理。

 



试剂盒组成


组成品

状态

包装

OVA包被96孔板(干燥板)

清洗后使用

96 wells(8×12)/1个

标准溶液(Anti OVA- IgG1:1,200U/mL)(单抗)

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgG1抗体(单抗)

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4

※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


样本信息

● 小鼠血清·血浆

● 10 μL/well(稀释样本)

※ 样本必须用附带的缓冲液稀释100倍以上。

 


◆测定范围


1.88~120 mU/mL(标准曲线范围)

(本试剂盒中1 U/mL定义为抗原结合常数(Ka)为6.9×107 M-1的抗体160ng/mL)

 


实验数据


精密度实验(实验内变化)


样本

A

B

1

105

18.2

2

100

16.9

3

96.2

16.7

4

100

17.4

5

106

17.4

Mean

101

17.3

SD

4.0

0.59

CV(%)

4.0

3.4

单位:mU/mL



重复性实验(实验间变化)


测定日/样本

C

D

E

0天

60.1

15.0

3.75

1天

58.5

14.7

3.70

2天

59.9

15.3

3.83

3天

61.4

15.7

3.82

Mean

60.0

15.2

3.77

SD

1.2

0.44

0.06

CV(%)

2.0

2.9

1.6

单位:mU/mL



添加回收实验


样本F


添加量

实测值

回收量

回收率(%)

0.00

8.86

7.29

15.8

6.94

95.2

11.0

19.5

10.6

96.4

14.6

23.0

14.1

96.6

单位:mU/mL,n=3


样本G


添加量

实测值

回收量

回收率(%)

0.00

51.5

26.9

76.8

25.3

97.1

31.4

80.6

29.1

92.5

47.1

100

48.5

103

单位:mU/mL,n=3



稀释直线性实验


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.9994~0.9998

参考文献



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Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses. Y. Nakagawa., H. Takamatsu., T. Okuno., S. Kang., S. Nojima., T. Kimura., T. R. Kataoka., M. Ikawa., T. Toyofuku., I. Katayama., and A. Kumanogoh. The Journal of Immunology, March 1, 2011 vol. 186 no. 5 2881-2888


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Suppression of ovalbumin-induced allergic diarrhea by diminished intestinal peristalsis in RAMP1-deficient mice. R. Yoshikawa., N. Mikami., I. Otani., T. Kishimoto., S. Nishioka., N. Hashimoto., Y. Miyagi., Y. Takuma., K. Sueda., S. Fukada., H. Yamamoto., K. Tsujikawa. Biochemical and Biophysical Research Communications, Vol. 410, Issue 3, 8 July 2011, Pages 389-393


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Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model. H.-J. Kim., H. J. Lee., S.-J. Jeong., H.-J. Lee., S.-H. Kim., E.-J. Park. Journal of Ethnopharmacology, Vol. 138, Issue 1, 31 October 2011, Pages 40-46


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Urban particulate matter in Beijin,China,enhances allergen-induced murine lung eosinophilia. He,M.,Ichinose,T.,Yoshida,S.,Nishikawa,M.,Mori,I.,Yanagisawa,R.,Takano,H.,Inoue,K.,Sun,G.,Shibamoto,T. Inhalation Toxicology 22(9):709-718,August,2010


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Deficiency in the Serum-Derived Hyaluronan-Associated Protein-Hyaluronan Complex Enhances Airway Hyperresponsiveness in a Murine Model of Asthma. L. Zhu., L. Zhuo., K. Kimata., E. Yamaguchi., H. Watanabe., M. A. Aronica., V. C. Hascall., K. Baba. Int Arch Allergy Immunol 153:223-233 2010


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Thioredoxin suppresses airway inflammation independently of systemic Th1/Th2 immune modulation. M. Torii., L. Wang., N. Ma., K. Saito., T. Hori., M. Sato-Ueshima., Y. Koyama., H. Nishikawa., N. Katayama., A. Mizoguchi., H. Shiku., J. Yodoi., K. Kuribayashi., T. Kato. European Journal of Immunology Vol.40(3) 787-796 2010


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Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Yanase,Y.,Hiragun,T.,Uchida,K.,Ishii,K.,Oomizu,S.,Suzuki,H.,Mihara,S.,Iwamoto,K.,Matsuo,H.,Onishi,N.,Kameyoshi,Y.,and Hide,M. Biochemical and Biophysical Research Communications 387:3:435- 439,2009


49.

Peroxisome Proliferator-Activated Receptor gNegatively Regulates Allergic Rhinitis in Mice. Fukui,N.,Honda,K.,Ito,E.,and Ishikawa K. Allergology Internatioal.58:247-253,2009


50.

IL-16 Variabillity and Modulation by Antiallergic Drugs in a Murine Experimental Allergic Rhinitis Model. Akiyama,K.,Karaki,M.,Kobayshi,R.,Dobashi,H.,Ishida,T, and Mori,N. Allergy and Immunology 149:4,2009


51.

Frequency of Foxp3+CD4+CD25+ T cell is associated with the phenotypes of allergic asthma. Matsumoto,K.,Inoue,H.,Fukuyama,A.,Kan,O,K.,Eguchi,T,M.,Matsumoto,T.,Moriwaki,A.,Nakano,T., and Nakanishi,Y. Respirology 14:2,2009


52.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T Cells in Th2-Type Airway Inflammation. Saito, K., Torii, M., Ning Ma, Tsuchiya, T., Wang, L., Hori, T., Nagakubo, D., Nitta, N.,Kanegasaki, S., Hieshima, K., Yoshie, O., Gabazza, E.C., Katayama, N., Shiku, H.,Kuribayashi, K. and Kato, T. The Journal of Immunology, 181:6889-6897, 2008


53.

Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008


54.

The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation. Ichinose,T., Yoshida,S., Hiyoshi,K., Sadakane,K., Takano,H., Nishikawa,M., Mori,I., Yanagisawa,R., Kawazato,H., Yasuda,A., and Shibamoto,T. Arch Environ Contam Toxicol 55:348-357,2008


55.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008


产品编号 产品名称 产品规格 产品等级
630-07669 (AKRIE-040)小鼠卵清蛋白特异性免疫球蛋白G1(OVA-IgG1) ELISA试剂盒
LBIS® OVA-IgG1 Mouse
96 tests

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒 LBIS® OVA-IgE Mouse

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒                              LBIS® OVA-IgE Mouse小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒

 


  IgE(Immunoglobulin E,免疫球蛋白E)是第五个被发现的免疫球蛋白,由5个结构域(VH、CHε 1~4)构成的两个Hε链和两个L链中组成的IgE,其分子量约190000,电泳实验中向γ1域移动。IgE的代谢半衰期约3天,正常人血清中的IgE浓度非常低,约300 ng/mL。但在寄生虫感染和枯草热时浓度会升高。与过敏性相关的IgE被称作反应素。因接触过敏原导致反应素含量升高,反应素会在Fc域与存在于皮肤、呼吸道、消化脏器中嗜碱性粒细胞和肥大细胞的FcεR1受体结合,引起细胞过敏。在结合过敏原后细胞发生脱粒现象,组胺,五羟色胺,蛋白酶,肝素,趋化因子,前列腺素,白三烯等被投放,经过支气管收缩和黏膜水肿,分泌亢进,从而诱发支气管哮喘,部分荨麻疹,过敏性鼻炎,过敏性反应等I型过敏性反应。

  本试剂盒是以OVA(卵清蛋白)作为免疫的抗原,在一个简化的反应系统中通过测定鼠抗OVA- IgE抗体值来进行小鼠免疫系统检测的试剂盒。

◆特点

LBIS® 小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE)ELISA试剂盒                              LBIS® OVA-IgE Mouse


● 测定时间短(总反应时间:1小时50分钟)。

● 微量样本即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

● 操作简便,无需进行特殊前处理。

 

试剂盒组成


组成品

状态

包装

OVA包被96孔板(干燥板)

清洗后使用

96 wells(8×12)/1个

标准溶液(Anti OVA-IgE:1,200 U/mL)(单抗)

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗小鼠IgE抗体(单抗)

稀释后使用

200 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

200 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4)※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

3个

产品说明书

1本

 


◆样本信息


● 小鼠血清·血浆

● 10 μL/well(稀释样本)

※ 样本需要用试剂盒附带的缓冲液调至标准曲线范围内。

※ 样本必须稀释10倍以上。

 


测定范围


1.88~120 U/mL(标准曲线范围)

(本试剂盒中1 U/mL定义为抗原结合常数(Ka)为2.0×108 M-1的抗体1.3 ng/mL)

 


实验数据


精密度实验(实验内变化)


样本

A

B

1

70.7

19.1

2

71.0

18.7

3

77.1

19.6

4

74.3

19.7

5

72.9

18.9

Mean

73.2

19.2

SD

2.6

0.42

CV(%)

3.6

2.2

单位:U/mL



重复性实验(实验间变化)


测定日/样本

C

D

E

0天

60.0

15.0

3.75

1天

59.1

15.0

3.75

2天

58.1

14.7

3.65

3天

63.6

16.0

3.48

Mean

60.2

15.2

3.66

SD

2.4

0.57

0.13

CV(%)

4.0

3.7

3.4

单位:U/mL



添加回收实验


样本F


添加量

实测值

回收量

回收率(%)

0.00

6.93

5.35

12.2

5.27

98.5

10.7

17.8

10.9

102

17.1

25.1

18.2

106

单位:U/mL,n=3


样本G


添加量

实测值

回收量

回收率(%)

0.00

40.5

30.8

70.4

29.5

95.8

35.9

77.1

36.6

102

53.9

94.9

54.4

101

单位:U/mL,n=3



稀释直线性实验


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.9987~0.9999

参考文献



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Pinocembrin attenuates allergic airway inflammation via inhibition of NF-κB pathway in mice. Gu X, Zhang Q, Du Q, Shen H, Zhu Z. Int Immunopharmacol. 2017 Oct 18;53:90-95.


 2.

A dichloromethane fraction of Triticum aestivum sprouts reduces allergic immune response through inhibiting Th2 differentiation in ovalbumin‑immunized mice. Ki HH, Hwang SW, Lee JH, Kim YH, Kim DK, Lee YM. Mol Med Rep. 2017 Sep;16(3):3535-3541.


 3.

Urban PM2.5 exacerbates allergic inflammation in the murine lung via a TLR2/TLR4/MyD88-signaling pathway. He M, Ichinose T, Yoshida Y, Arashidani K, Yoshida S, Takano H, Sun G, Shibamoto T. Sci Rep. 2017 Sep 8;7(1):11027.


 4.

Activation of group 2 innate lymphoid cells exacerbates and confers corticosteroid resistance to mouse nasal type 2 inflammation. Morikawa T, Fukuoka A, Matsushita K, Yasuda K, Iwasaki N, Akasaki S, Fujieda S, Yoshimoto T. Int Immunol. 2017 May 1;29(5):221-233.


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Aquaporin-3 potentiates allergic airway inflammation in ovalbumin-induced murine asthma. Ikezoe K, Oga T, Honda T, Hara-Chikuma M, Ma X, Tsuruyama T, Uno K, Fuchikami J, Tanizawa K, Handa T, Taguchi Y, Verkman AS, Narumiya S, Mishima M, Chin K. Sci Rep. 2016 May 11;6:25781.


 6.

Exposure to bisphenol A enhanced lung eosinophilia in adult male mice. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Shibamoto T, Sun G. Allergy Asthma Clin Immunol. 2016 Apr 14;12:16.


 7.

Differences in allergic inflammatory responses between urban PM2.5 and fine particle derived from desert-dust in murine lungs. He M, Ichinose T, Kobayashi M, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Toxicol Appl Pharmacol. 2016 Apr 15;297:41-55.


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Desert dust induces TLR signaling to trigger Th2-dominant lung allergic inflammation via a MyD88-dependent signaling pathway. He M, Ichinose T, Song Y, Yoshida Y, Bekki K, Arashidani K, Yoshida S, Nishikawa M, Takano H, Shibamoto T, Sun G. Toxicol Appl Pharmacol. 2016 Apr 1;296:61-72.


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Administration of Pigment Epithelium-Derived Factor Inhibits Airway Inflammation and Remodeling in Chronic OVA-Induced Mice via VEGF Suppression. Zha W, Su M, Huang M, Cai J, Du Q. Allergy Asthma Immunol Res. 2016 Mar;8(2):161-9.


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Low-dose benzo[a]pyrene aggravates allergic airway inflammation in mice. Yanagisawa R, Koike E, Win-Shwe TT, Ichinose T, Takano H. J Appl Toxicol. 2016 Feb 25.


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Prevention of allergic rhinitis by ginger and the molecular basis of immunosuppression by 6-gingerol through T cell inactivation. Kawamoto Y, Ueno Y, Nakahashi E, Obayashi M, Sugihara K, Qiao S, Iida M, Kumasaka MY, Yajima I, Goto Y, Ohgami N, Kato M, Takeda K. J Nutr Biochem. 2016 Jan;27:112-22.


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Immunotoxic Effect of Low-Dose Methylmercury Is Negligible in Mouse Models of Ovalbumin or Mite-Induced Th2 Allergy. Nakamura R, Takanezawa Y, Sone Y, Uraguchi S, Sakabe K, Kiyono M.

Biol Pharm Bull. 2016;39(8):1353-8.


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Prevention of allergic rhinitis by ginger and the molecular basis of immunosuppression by 6-gingerol through T cell inactivation. Yoshiyuki Kawamoto, Yuki Ueno, Emiko Nakahashi, Momoko Obayashi, Kento Sugihara, Shanlou Qiao, Machiko Iida, Mayuko Y. Kumasaka, Ichiro Yajima, Yuji Goto, Nobutaka Ohgami, Masashi Kato, Kozue Takeda. The Journal of Nutritional Biochemistry, Volume 27, Jan. 2016, Pages 112–122


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Effects of Sohamhyoong-Tang on Ovalbumin-Induced Allergic Reaction in BALB/c Mice. Jo SH, Lee YJ, Kang DG, Lee HS, Kim DK, Park MC. Evid Based Complement Alternat Med. 2016;2016:6286020.


15.

Effect of diosmetin on airway remodeling in a murine model of chronic asthma. Ge A, Liu Y, Zeng X, Kong H, Ma Y, Zhang J, Bai F, Huang M. Acta Biochim Biophys Sin (Shanghai). Vol.47(8), p604-11, Aug 2015.


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PM2.5-rich dust collected from the air in Fukuoka, Kyushu, Japan, can exacerbate murine lung eosinophilia. He M, Ichinose T, Ren Y, Song Y, Yoshida Y, Arashidani K, Yoshida S, Nishikawa M, Takano H, Sun G. Inhal Toxicol. Vol.27(6), p287-99, May 2015.


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Anti-asthma potential of crocin and its effect on MAPK signaling pathway in a murine model of allergic airway disease. Xiong Y, Wang J, Yu H, Zhang X, Miao C. Immunopharmacol Immunotoxicol. Vol.10, p1-8, Mar 2015.


18.

Roles of lipoxin A4 receptor activation and anti-interleukin-1β antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-κB pathway in airway inflammation induced by ovalbumin. Kong X, Wu SH, Zhang L, Chen XQ. Mol Med Rep. 2015 Mar 5


19.

Pharyngeal aspiration of metal oxide nanoparticles showed potential of allergy aggravation effect to inhaled ovalbumin. Horie M, Stowe M, Tabei M, Kuroda E. Inhal Toxicol. Vol.27(3), p181-90, Feb 2015.


20.

Oxidized dietary oils enhance immediate- and/or delayed-type allergic reactions in BALB/c mice. Ogino H, Sakazaki F, Okuno T, Arakawa T, Ueno H. Allergol Int. Vol.64(1), p66-72, Jan 2015.


21.

The effects of nodakenin on airway inflammation, hyper-responsiveness and remodeling in a murine model of allergic asthma. Xiong Y, Wang J, Yu H, Zhang X, Miao C, Ma S. Immunopharmacol Immunotoxicol. Vol.36(5), p341-348, Oct 2014.


22.

Allogeneic pluripotent stem cells suppress airway inflammation in murine model of acute asthma. Ogulur I, Gurhan G, Kombak FE, Filinte D, Barlan I, Akkoc T. International Immunopharmacology, Vol.22(1), p31-40 Sep 2014.


23.

Effects of prior oral exposure to combinations of environmental immunosuppressive agents on ovalbumin allergen-induced allergic airway inflammation in Balb/c mice. Fukuyama T, Nishino R, Kosaka T, Watanabe Y, Kurosawa Y, Ueda H, Harada T.. Immunopharmacol Immunotoxicol. Vol.36(4), p261-70, Aug 2014.


24.

Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust. Ren Y, Ichinose T, He M, Song Y, Yoshida Y, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Jun 2014.


25.

A bacterial extract of OM-85 Broncho-Vaxom prevents allergic rhinitis in mice. Han L, Zheng CP, Sun YQ, Xu G, Wen W, Fu QL. American Journal of Rhinology & Allergy, Vol.28(2), p110-116, Mar-Apr 2014.


26.

Broncho-Vaxom Attenuates Allergic Airway Inflammation by Restoring GSK3β-Related T Regulatory Cell Insufficiency. Fu R, Li J, Zhong H, Yu D, Zeng X, Deng M, Sun Y, Wen W, Li H. PLoS One. 2014 Mar 25;9(3):e92912


27.

Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin-induced lung eosinophilia by ASD and the fungus in mice. Liu B, Ichinose T, He M, Kobayashi F, Maki T, Yoshida S, Yoshida Y, Arashidani K, Takano H, Nishikawa M, Sun G, Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.10(1), Feb 2014.


28.

Midazolam inhibits IgE production in mice via suppression of class switch recombination. Kusama H, Kobayashi R, Kurita-Ochiai T. Journal of Oral Science, Vol.56(1), p77-83, 2014.


29.

Induction of immune tolerance and reduction of aggravated lung eosinophilia by co-exposure to Asian sand dust and ovalbumin for 14 weeks in mice. He M., Ichinose T., Yoshida S., Takano H., Nishikawa M., Sun G. and Shibamoto T. Allergy, Asthma & Clinical Immunology, Vol.9(19), 2013.


30.

Galangin Abrogates Ovalbumin-Induced Airway Inflammation via Negative Regulation of NF-B. Zha W-J., Qian Y., Shen Y., Du Q., Chen F-F., Wu Z-Z., Li X. and Huang M. Evidence-Based Complementary and Alternative Medicine, Vol.2013 (2013), p14.


31.

Effects of two Asian sand dusts transported from the dust source regions of Inner Mongolia and northeast China on murine lung eosinophilia. M.He, T.Ichinose, Y.Song, Y.Yoshida, K.Arashidani, S.Yoshida, B.Liu, M.Nishikawa, H.Takano, G.Sun. Toxicology and Applied Pharmacology, Available online 26 July 2013.


32.

Effect of the size of receptor in allergy detection using field effect transistor biosensor. S.Hideshima, S.Kuroiwa, M.Kimura, S.Cheng, T.Osaka. Electrochimica Acta, Available online 24 July 2013.


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Elevated Macrophage Inflammatory Protein 1α and Interleukin-17 Production in an Experimental Asthma Model Infected with Respiratory Syncytial Virus. T.Ishioka, Y.Yamada, H.Kimura, M.Yoshizumi, H.Tsukagoshi, K.Kozawa, K.Maruyama, Y.Hayashi, M.Kato. Int Arch Allergy Immunol, Vol.161(suppl 2), p129-137, May 2013.


34.

Leukotriene B4 receptor BLT2 negatively regulates allergic airway eosinophilia . Y.Matsunaga, S.Fukuyama, T.Okuno, F.Sasaki, T. Matsunobu, Y.Asai, K.Matsumoto, K.Saeki, M.Oike, Y.Sadamura, K.Machida, Y.Nakanishi, M.Kubo, T.Yokomizo and H.Inoue. The FASEB Journal, Published online before print April 19, 2013.


35.

Effects of exposure to nanoparticle-rich or -depleted diesel exhaust on allergic pathophysiology in the murine lung. Tanaka M., Aoki Y., Takano H., Fujitani Y., Hirano S., Nakamura R., Sone Y., Kiyono M., Ichinose T., Itoh T., Inoue K. Journal of Toxicological Sciences, Vol.38(1), p35-48, Feb 2013.


36.

HIF-1α Inhibition Reduces Nasal Inflammation in a Murine Allergic Rhinitis Model. Zhou H, Chen X, Zhang W-M, Zhu L-P, Cheng L. PLOS one, 2012.


37.

Human Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Prevent Allergic Airway Inflammation in Mice. Sun Y-Q, Deng M-X, He J, Zeng Q-X, Wen W, Wong D S.H, Tse H-F, Xu G, Lian Q, Shi J, Fu Q-L. STEM CELLS, Vol.30(12), p2692-2699, Dec 2012.


38.

Aggravating effects of Asian sand dust on lung eosinophilia in mice immunized beforehand by ovalbumin. He M, Ichinose T, Yoshida S, Takano H, Nishikawa M, Mori I, Sun G, Shibamoto T. Inhalation Toxicology, Vol.24(11) , p751-761, Sep 2012.


39.

Attenuation of airway hyperreactivity and T helper cell type 2 responses by coumarins from Peucedanum praeruptorum Dunn in a murine model of allergic airway inflammation. Xiong Y-Y, Wu F-H, Wang J-S, Li J, Kong L-Y. Journal of Ethnopharmacology, Vol.141(1), p314-321, May 2012.


40.

Effects of lysed Enterococcus faecalis FK-23 on experimental allergic rhinitis in a murine model. Zhu L,Shimada T, Chen R, Lu M, Zhang Q, Lu W, Yin M, Enomoto T, Cheng L. Journal of Biomedical Research, Vol.26(3), p226-234, May 2012.


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Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy. S. C. Diesner., A. Olivera., S. Dillahunt., C. Schultz., T. Watzlawek., E. Forster-Waldl., A. Pollak., E. Jensen-Jarolim., E. Untersmayr., J. Rivera. Immunology Letters, Vol. 141, Issue 2, 30 January 2012, Pages 210-219


42.

Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses. Y. Nakagawa., H. Takamatsu., T. Okuno., S. Kang., S. Nojima., T. Kimura., T. R. Kataoka., M. Ikawa., T. Toyofuku., I. Katayama., and A. Kumanogoh. The Journal of Immunology, March 1, 2011 vol. 186 no. 5 2881-2888


43.

Suppression of ovalbumin-induced allergic diarrhea by diminished intestinal peristalsis in RAMP1-deficient mice. R. Yoshikawa., N. Mikami., I. Otani., T. Kishimoto., S. Nishioka., N. Hashimoto., Y. Miyagi., Y. Takuma., K. Sueda., S. Fukada., H. Yamamoto., K. Tsujikawa. Biochemical and Biophysical Research Communications, Vol. 410, Issue 3, 8 July 2011, Pages 389-393


44.

Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model. H.-J. Kim., H. J. Lee., S.-J. Jeong., H.-J. Lee., S.-H. Kim., E.-J. Park. Journal of Ethnopharmacology, Vol. 138, Issue 1, 31 October 2011, Pages 40-46


45.

Urban particulate matter in Beijin,China,enhances allergen-induced murine lung eosinophilia. He,M.,Ichinose,T.,Yoshida,S.,Nishikawa,M.,Mori,I.,Yanagisawa,R.,Takano,H.,Inoue,K.,Sun,G.,Shibamoto,T. Inhalation Toxicology 22(9):709-718,August,2010


46.

Deficiency in the Serum-Derived Hyaluronan-Associated Protein-Hyaluronan Complex Enhances Airway Hyperresponsiveness in a Murine Model of Asthma. L. Zhu., L. Zhuo., K. Kimata., E. Yamaguchi., H. Watanabe., M. A. Aronica., V. C. Hascall., K. Baba. Int Arch Allergy Immunol 153:223-233 2010


47.

Thioredoxin suppresses airway inflammation independently of systemic Th1/Th2 immune modulation. M. Torii., L. Wang., N. Ma., K. Saito., T. Hori., M. Sato-Ueshima., Y. Koyama., H. Nishikawa., N. Katayama., A. Mizoguchi., H. Shiku., J. Yodoi., K. Kuribayashi., T. Kato. European Journal of Immunology Vol.40(3) 787-796 2010


48.

Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization. Yanase,Y.,Hiragun,T.,Uchida,K.,Ishii,K.,Oomizu,S.,Suzuki,H.,Mihara,S.,Iwamoto,K.,Matsuo,H.,Onishi,N.,Kameyoshi,Y.,and Hide,M. Biochemical and Biophysical Research Communications 387:3:435- 439,2009


49.

Peroxisome Proliferator-Activated Receptor gNegatively Regulates Allergic Rhinitis in Mice. Fukui,N.,Honda,K.,Ito,E.,and Ishikawa K. Allergology Internatioal.58:247-253,2009


50.

IL-16 Variabillity and Modulation by Antiallergic Drugs in a Murine Experimental Allergic Rhinitis Model. Akiyama,K.,Karaki,M.,Kobayshi,R.,Dobashi,H.,Ishida,T, and Mori,N. Allergy and Immunology 149:4,2009


51.

Frequency of Foxp3+CD4+CD25+ T cell is associated with the phenotypes of allergic asthma. Matsumoto,K.,Inoue,H.,Fukuyama,A.,Kan,O,K.,Eguchi,T,M.,Matsumoto,T.,Moriwaki,A.,Nakano,T., and Nakanishi,Y. Respirology 14:2,2009


52.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T Cells in Th2-Type Airway Inflammation. Saito, K., Torii, M., Ning Ma, Tsuchiya, T., Wang, L., Hori, T., Nagakubo, D., Nitta, N.,Kanegasaki, S., Hieshima, K., Yoshie, O., Gabazza, E.C., Katayama, N., Shiku, H.,Kuribayashi, K. and Kato, T. The Journal of Immunology, 181:6889-6897, 2008


53.

Effects of Asian Sand Dust, Arizona Sand Dust, Amorphous Silica and Aluminum Oxide on Allergic Inflammation in the Murine Lung. Ichinose, T., Yoshida, S., Sadakane, K., Takano, H., Yanagizawa, R., Inoue, K., Nishikawa, M.,Mori, I., Kawazato, H., Yasuda, A. and Shibamoto, T. Inhalation Toxicology, Volume 20, Issue 7, 685-694, 2008


54.

The Effects of Microbial Materials Adhered to Asian Sand Dust on Allergic Lung Inflammation. Ichinose,T., Yoshida,S., Hiyoshi,K., Sadakane,K., Takano,H., Nishikawa,M., Mori,I., Yanagisawa,R., Kawazato,H., Yasuda,A., and Shibamoto,T. Arch Environ Contam Toxicol 55:348-357,2008


55.

Differential Regulatory Function of Resting and Preactivated Allergen-Specific CD4+CD25+ Regulatory T cell in Th2-Type Airway Inflammation. Saito,K., Torii,M., Ma,N., Tsuchiya,T., Wang,L.,Hori,T., Nagakubo,D., Nitta,N., Kanegasaki,S., Hieshima,K., Yoshie,O., Gabazza,E,C., Katayama,N., Shiku,H., Kuribayashi,K., and Kato,T. The Journal of Immunology 181:6889-6897,2008


产品编号 产品名称 产品规格 产品等级
639-07651 (AKRIE-030)小鼠卵清蛋白特异性免疫球蛋白E(OVA-IgE) ELISA试剂盒
 LBIS® OVA-IgE Mouse
96 tests

大鼠促黄体生成素(LH)ELISA试剂盒(S型) LBIS® Rat LH ELISA KIT(S type)

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

大鼠促黄体生成素(LH)ELISA试剂盒(S型)                              LBIS® Rat LH ELISA KIT(S type)大鼠促黄体生成素(LH)ELISA试剂盒(S型)

 


  LH是在垂体前叶(腺垂体)嗜碱性促性腺激素生产细胞(脑垂体)中通过与FSH共同产生、贮存在下丘脑激素LHRH的刺激下而分泌的。精巢中也含有这类物质。在动物种类方面分布在鱼类-哺乳类的全脊椎动物。在雄性大鼠垂体中LH含量为6~7 μg/gland,雌性为3~4 μg/gland(NIH-LH1 S1 换算)。雄性较多,雌性会根据性周期而变化。

  LH是分子量约29000的糖蛋白,是由TSH、FSH共同的α-亚基以及LH特有的β-亚基组成的杂二聚体。

  LH的目标器官,在雌性动物中是卵泡的成熟颗粒细胞,与FSH协同促进卵细胞成熟和雌性激素的生产,诱导排卵,促进排卵后黄体化孕甾酮生产分泌。在雄性动物中是精巢的间质细胞(睾丸间质细胞),促进其雄性激素分泌,与雄性激素作用促进2次精子形成有关。受体是膜7次贯通- G蛋白共轭型PKA。

  因此,LH不足会导致性类固醇分泌降低,间质细胞萎缩,排卵黄体化停止等现象。过量状态下会导致精巢间质细胞肥大而且持续萎缩,雌激素、雄激素分泌增加,早熟过排,促性成熟等现象。

  LH的分泌受GnRH (LHRH)直接促进,生理状态表现为血液中性类固醇低下(间接、直接),性周期变化(特别是排卵前期),更年期-闭经后分泌增加。

  男性会随着年龄的增长而增加。发情前期大量分泌的雌激素通过正反馈,在LHRH作用下引起暂时性LH大量分泌(LH波动),诱发排卵。

  血液中性类固醇的增加以及阿片肽特别是β-内啡肽,在幼儿期、妊娠期、产后期等会抑制LH的分泌。

 


◆特点


● 测定时间短(总反应时间:3小时50分钟)。

● 微量样本(标准操作法10 μL)即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

 


试剂盒组成


组成品

状态

包装

(A)抗体包被96孔板

清洗后使用

96 wells(8×12)/1个

(B)标准溶液(100 ng/mL)

稀释后使用

200 μL/1瓶

(C)测定用缓冲液

直接使用

60 mL/1瓶

(D)生物素结合抗GH抗体

稀释后使用

100 μL/1瓶

(E)过氧化物酶·抗生素结合物

稀释后使用

100 μL/1瓶

(F)显色液(TMB)

直接使用

12 mL/1瓶

(G)样本稀释用缓冲液

直接使用

12 mL/1瓶

(H)终止液(1M H2SO4

※小心轻放

直接使用

12 mL/1瓶

(I)浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

4个

产品说明书

1本

 


样本信息


● 大鼠血清·血浆

● 10 μL/well(标准操作法)

※ 样本需要用(G)样本稀释缓冲液稀释2倍,搅拌后室温下静置处理10分钟,再用(C)测定缓冲液稀释2.5倍,稀释液在测定中使用(最终稀释

      率为5倍)。最终稀释倍数的极限是2.5倍。但是,需用缓冲液将板孔总量调制至 50 μL

※ 推荐使用1 mg/mL(终浓度)EDTA-2Na作为抗凝剂,不可使用肝素Na血浆。

 


测定范围


0.313~10 ng/mL(标准曲线范围)

1.565~50 ng/mL(样本量10 μL时)

 


实验数据


精密度实验(实验内变化)


样本

A

B

1

8.74

1.15

2

8.78

1.03

3

8.79

1.07

4

8.68

1.06

5

8.03

1.03

6

8.27

1.13

7

7.94

1.07

8

7.97

1.05

Mean

8.40

1.07

SD

0.39

0.04

CV(%)

4.6

3.9

单位:ng/mL,n=8


重复性实验(实验间变化)


测定日/样本

E

F

G

0天

5.01

1.25

0.328

1天

5.01

1.24

0.329

2天

5.00

1.27

0.347

3天

5.00

1.28

0.337

Mean

5.00

1.26

0.335

SD

0.0019

0.016

0.0088

CV(%)

0.038

1.3

2.6

单位:ng/mL,n=4


添加回收实验


样本C


添加量

实测值

回收量

回收率(%)

0.00

1.29

0.584

1.89

0.601

103

1.17

2.48

1.19

102

1.75

3.01

1.73

98.5

单位:ng/mL,n=2


样本D


添加量

实测值

回收量

回收率(%)

0.00

2.23

1.36

3.59

1.35

100

3.39

5.60

3.36

99.2

4.07

6.21

3.97

97.7

单位:pg/mL,n=2


稀释直线性实验


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值 R2=0.999。

参考文献

 1.

Easy detection of hormone secretion from LβT2 cells by using Gaussia luciferase.Satou K, Mochimaru Y, Nakakura T, Kusada T, Negishi J, Musha S, Yoshimura N, Kato Y, Tomura H.J Reprod Dev. 2017 Apr 21;63(2):199-204.


 2.

α-Lipoic acid potentially targets AMP-activated protein kinase and energy production in the fetal brain to ameliorate dioxin-produced attenuation in fetal steroidogenesis.Takeda T, Matsuo Y, Nishida K, Fujiki A, Hattori Y, Koga T, Ishii Y, Yamada H.J Toxicol Sci. 2017;42(1):13-23.


 3.

Kisspeptin expression is decreased in the arcuate nucleus of hypothyroid female rats with irregular estrus cycles.Tomori Y, Takumi K, Iijima N, Takai S, Ozawa H.Neurosci Res. 2016 Nov 27.


 4.

Increase of kisspeptin-positive cells in the hypothalamus of a rat model of polycystic ovary syndrome. Kondo M, Osuka S, Iwase A, Nakahara T, Saito A, Bayasula, Nakamura T, Goto M, Kotani T, Kikkawa F Metab Brain Dis. 2016 Jun;31(3):673-81.


 5.

The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASIa, A.A. Momoha, N.A. Awololac, O.E. Kalea, b, W.O. Okunowod Toxicology Reports,Vol.3, 2016, Pages 620–627


 6.

Hormone Inhibition During Mini-Puberty and Testicular Function in Male Rats. Chen L, Wang R, Wang W, Lu W, Xiao Y, Wang D, Dong Z. Int J Endocrinol Metab. 2015 Oct 3;13(4):e25465


 7.

Increase of kisspeptin-positive cells in the hypothalamus of a rat model of polycystic ovary syndrome. Kondo M, Osuka S, Iwase A, Nakahara T, Saito A, Bayasula, Nakamura T, Goto M, Kotani T, Kikkawa F Metab Brain Dis. 2016 Feb 11. [Epub ahead of print


 8.

Oleuropein supplementation increases urinary noradrenaline and testicular testosterone levels and decreases plasma corticosterone level in rats fed high-protein diet
Oi-Kano Y., Kawada T., Watanabe T., Koyama F., Watanabe K., Senbongi R., Iwai K.The Journal of Nutritional Biochemistry, Vol.24(5), p887-893, May 2013.


 9.

Multiple Cholinergic Signaling Pathways in Pituitary Gonadotrophs Zemkova H., Kucka M., Bjelobaba IV., Tomi M. and Stojilkovic SS. Endocrinology, Vol.154(1), p421-433, Jan 2013.


10.

ERα/E2 signaling suppresses the expression of steroidogenic enzyme genes via cross-talk with orphan nuclear receptor Nur77 in the testes S.-Y. Lee., E. Park., S.-C. Kim., R.-S. Ahn., C. Ko., K. Lee. Molecular and Cellular Endocrinology, 2012.


11.

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells Son YL., Ubuka T., Millar RP., Kanasaki H. and Tsutsui K. Endocrinology, Vol.153(5), p2332-2343, May 2012.


12.

Anti-androgenic effects of S-40542, a novel non-steroidal selective androgen receptor modulator (SARM) for the treatment of benign prostatic hyperplasia H. Nejishima., N. Yamamoto., M. Suzuki., K. Furuya., N. Nagata., S. Yamada. The Prostate published online:16 MAR 2012


13.

Gonadotropin-Inhibitory Hormone Inhibits GnRH-Induced Gonadotropin Subunit Gene Transcriptions by Inhibiting AC/cAMP/PKA-Dependent ERK Pathway in LβT2 Cells Y. L. Son., T. Ubuka., R. P. Millar., H. Kanasaki., and K. Tsutsui. Endocrinology, Feb 28, 2012


14.

Neonatal immune challenge alters reproductive development in the female rat L. Sominsky., C.L. Meehan., A.K. Walker., L. Bobrovskaya., E. A. McLaughlin., D. M. Hodgson. Hormones and Behavior, 2012.

产品编号 产品名称 产品规格 产品等级
630-23929 (AKRLH-010S)大鼠促黄体生成素(LH)ELISA试剂盒(S型)
LBIS®  Rat LH ELISA KIT(S type)
96 tests

LBIS® 大鼠生长激素(GH) ELISA试剂盒 LBIS® Rat GH ELISA Kit

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LBIS® 大鼠生长激素(GH) ELISA试剂盒                              LBIS® Rat GH ELISA Kit

大鼠生长激素(GH) ELISA试剂盒



  生长激素(Growth hormone,别名Somatotrophic hormone、 STH、 Somatotrop(h)in)主要是由垂体前叶嗜酸性腺垂体分泌的蛋白激素,在大脑和淋巴细胞表达。与GH高度相似的GH2表达于人的胎盘。GH作用于肝脏肌肉肾脏软骨细胞成纤维细胞胸腺上皮细胞。在IGF-1作用下,通过软骨细胞的增殖、硫酸软骨素的合成、肝脏其他器官细胞肥大增殖、促蛋白合成等促进生长,对胸腺细胞分泌胸腺素起到促进作用。GH暂时性表达胰岛素样作用,之后在脂肪细胞中通过脂肪分解增加游离脂肪酸、血糖上升、胰岛素拮抗作用抑制糖分解、肌肉中糖原含量增加、末梢组织胰岛素灵敏性下降等代谢方面起到一定双相性作用。还起到类似催乳素作用对Na、K、Mg、Ca、P的存积促进小肠Ca吸收乳腺发育乳汁分泌等作用。

GHRH生长素释放肽甲状腺激素皮质醇视黄酸均可促进GH合成分泌。另外通过胰高血糖素加压素2-脱氧-D-葡萄糖耐受性精氨酸等酸负载蛋白质摄入TF5β-内啡肽左旋多巴肾上腺素α受体刺激等可促进GH分泌。促进GH分泌的生理状态是低血糖应激(发热,外伤,出血,乙醚麻醉,精神焦虑)空腹运动慢波睡眠等。GH分泌会抑制引起促生长素抑制素(SRIF)活化素肾上腺素β受体刺激葡萄糖游离脂肪酸皮质类固醇投放高浓度IGF-1高浓度GH等现象发生。抑制GH分泌的生理状态是高血糖增加血液中的脂肪异相睡眠等。GH分泌是具有episodic性的。也就是说可间隔性地使血糖浓度急剧上升或下降。因此非人为采血时血中GH水平会变化很大。

◆特点

LBIS® 大鼠生长激素(GH) ELISA试剂盒                              LBIS® Rat GH ELISA Kit

● 测定时间短(总反应时间:5小时)

● 微量样本(标准操作法5 μL)即可测定。

● 使用无害的防腐剂。

● 全部试剂为溶液即用类型。

● 高测定精度和高重复性。

 

试剂盒组成


组成品

状态

包装

抗体包被96孔板

清洗后使用

96 wells(8×12)/1个

标准溶液(20 ng/mL)

稀释后使用

100 μL/1瓶

缓冲液

直接使用

60 mL/1瓶

生物素结合抗GH抗体

稀释后使用

100 μL/1瓶

过氧化物酶·抗生素结合物

稀释后使用

100 μL/1瓶

显色液(TMB)

直接使用

12 mL/1瓶

终止液(1M H2SO4

※小心轻放

直接使用

12 mL/1瓶

浓缩清洗液(10×)

稀释后使用

100 mL/1瓶

孔板密封膜

4个

产品说明书

1本

 


物种交叉性


2000 pg/mL时数据+:有交叉性   ―:无交叉性


动物种类

对象物质

反应性及反应率(%)

大鼠

r-GH

100

Prolactin

0.02

Placental   lactogen

0.02

TSH

LH

FSH

小鼠

GH

TSH

 


◆样本信息


● 大鼠血清·血浆

● 5 μL/well(标准操作法)

※ 样本量可调节范围:5~25 μL。但需用缓冲液将板孔总量调制至50 μL

※ 推荐使用1 mg/mL (终浓度)EDTA作为抗凝剂。

 


◆测定范围


● 31.3~2,000 pg/mL(标准曲线范围)

● 62.6~4,000 pg/mL(样本量25 μL时)

● 0.313~20 ng/mL(标准操作时)

 


◆实验数据


精密度实验(实验内变化)


样本

A

B

1

262

864

2

247

837

3

250

813

4

258

775

5

251

780

6

257

800

7

254

771

8

270

779

Mean

256

802

SD

7.19

33.5

CV(%)

2.8

4.2

单位:pg/mL



重复性实验(实验间变化)


测定日/样本

E

F

G

0天

1626

412

96.0

1天

1576

407

97.9

2天

1615

409

96.1

3天

1561

401

103

Mean

1595

407

98.3

SD

31.0

4.50

3.35

CV(%)

1.9

1.1

3.4

单位:pg/mL,n=4



添加回收实验


样本C


添加量

实测值

回收量

回收率(%)

0.00

101

155

265

164

106

192

285

184

95.8

223

325

224

100

单位:pg/mL,n=2


样本D


添加量

实测值

回收量

回收率(%)

0.00

506

303

822

316

104

466

949

443

95.1

539

1058

552

102

单位:pg/mL,n=2



稀释直线性实验


2个血清样本连续用稀释缓冲液稀释3个梯度测定结果,直线回归值R2=0.999。

参考文献

1.

Daily Fasting Blood Glucose Rhythm in Male Mice: A Role of the Circadian Clock in the Liver. Ando H, Ushijima K, Shimba S, Fujimura A. Endocrinology. 2016 Feb;157(2):463-9.


2.

Casted-immobilization downregulates glucocorticoid receptor expression in rat slow-twitch soleus muscle. Sato S., Suzuki H., Tsujimoto H., Shirato K., Tachiyashiki K., Imaizumia K. Life Sciences, Vol.89(25-26), p962-967, Dec 2011.


3.

Activation of PPARδ promotes mitochondrial energy metabolism and decreases basal insulin secretion in palmitate-treated β-cells. Jiang L., Wan J., Ke L., LU Q., Tong N. Molecular and Cellular Biochemistry, Vol.343(1-2), p249-256, Oct 2010.


4.

The CXCR4 antagonist AMD3100 suppresses hypoxia-mediated growth hormone production in GH3 rat pituitary adenoma cells. Yoshida,D.,Koketshu,K.,Nomura,R.,Teramoto,A. J Neurooncol, 2010.

产品编号 产品名称 产品规格 产品等级
639-13749 (AKRGH-010)LBIS® Rat GH ELISA Kit
LBIS® 大鼠生长激素(GH) ELISA试剂盒
96 tests

细胞因子ELISA试剂盒

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细胞因子ELISA试剂盒细胞因子ELISA试剂盒



细胞因子,包括白介素、干扰素和趋化因子,是细胞调节免疫、炎症和一些胚胎发育过程中所分泌的信号分子。细胞因子在血管生成和癌症中也发挥了重要作用。细胞因子具有多向性、重叠性、甚至有时相互对立的功能。这主要是与其浓度、作用的细胞类型,以及其他细胞因子和介质的存在有关。Enzo Life Sciences针对先天性和适应性免疫研究,可提供多种细胞因子抗体、蛋白、和试剂盒,包括多种高特异性的人和小鼠细胞因子ELISA试剂盒。

细胞因子ELISA试剂盒



◆产品列表

产品编号

产品名称

样品类型

灵敏度

检测范围

检测时间

规格

ADI-900-136

IFN-γ (human), ELISA kit

细胞培养上清、

血浆、血清和尿液

< 2 pg/mL 

 25.6-1,000 pg/mL

3 h

96 tests

ADI-900-150

IGF-1 (human), ELISA kit

血浆和血清。有文献检测

细胞培养上清和乳汁

34.2 pg/mL

187-6,000 pg/mL

4 h

96 tests

ADI-900-036

IL-10 (human), ELISA kit 

细胞培养上清、

血浆和血清

3.75 pg/mL 

 7.81-500 pg/mL

<3 h

96 tests

ADI-900-177

IL-17A (human), ELISA kit 

细胞培养上清、

血浆和血清

0.2 pg/mL

2.34-75   pg/mL

3 h

96 tests

ADI-900-201

IL-33 (human), ELISA kit

细胞裂解液、

细胞培养上清、

血清、血浆和滑液样本

1.7 pg/mL 

7.8-500 pg/mL

3 h

96 tests

ADI-900-043

 IL-4 (mouse), ELISA kit 

细胞培养上清和血清。

有文献检测血浆

4.34 pg/mL 

7.81-1,000 pg/mL

3 h

96 tests

ADI-900-045

IL-6 (mouse), ELISA kit

细胞培养上清和血清。

有文献检测组织样本

1.01 pg/mL

7.81-1,000 pg/mL

<3 h

96 tests

ADI-900-156

 IL-8 (human), ELISA kit 

细胞培养上清、

血浆和血清

0.64 pg/mL 

7.8-1,000   pg/mL

<3 h

96 tests

ADI-900-019A

Leptin (mouse), ELISA kit

细胞培养上清、血浆和血清。有文献检测组织样本

25.4 pg/mL 

50-3,200 pg/mL

h

96 tests

ADI-900-015A

Leptin (rat), ELISA kit

细胞培养上清、

血浆和血清

67.2 pg/mL 

100-6,400 pg/mL

3 h

96 tests

ADI-900-077

MCP-1 (rat), ELISA kit

细胞培养上清和血清

20.45 pg/mL 

50-3,200 pg/mL 

2 h

96 tests

BPD-KIT-036

NGAL (human) ELISA kit

尿液、血浆、血清、组织提取物和细胞培养上清

pg/mL

10-1000 pg/mL

<4 h

96 tests

ADI-900-155

TGF-β1 ELISA kit

细胞培养上清、

血浆和血清

3.3 pg/mL

31.25-1000 pg/mL 

4 h

96 tests

APO-54N-027-KI01

TL1A soluble (human), ELISA kit

细胞培养上清和血清

15 pg/mL 

39-2500 pg/mL

<3 h

96 tests

ADI-900-099

TNF-α(human), ELISA kit

细胞培养上清、

血浆和血清

8.43 pg/mL 

15.63-1,000 pg/mL 

4 h

96 tests

ADI-900-047

TNF-α(mouse), ELISA kit

细胞培养上清和血清。有文献检测组织样本和血浆

3.9 pg/mL

31.25-2,000 pg/mL 

5 h

96 tests

※ 本页面产品仅供研究用,研究以外不可使用。

产品编号 产品名称 产品规格 产品等级

ZBP1单抗(Zippy-1)

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ZBP1单抗(Zippy-1)

ZBP1单抗(Zippy-1)

◆产品描述


ZBP1是一种干扰素诱导蛋白,它与细胞质DNA结合并在肿瘤和病原体的宿主防御反应中发挥作用。ZBP1在淋巴组织中高表达,包括淋巴结、白细胞、扁桃体、骨髓和脾脏。ZBP1是甲型流感病毒(IAV)的先天免疫传感器。ZBP1调控NLRP3(nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3)炎症小体活性,在IAV感染期间诱导不同类型的细胞死亡(细胞凋亡,程序性细胞坏死和细胞焦亡)。

 


◆产品信息

产品详情

别名

 Z-DNA结合蛋白1;
 肿瘤基质和活化巨噬细胞蛋白DLM-1;
 DNA依赖性干扰素调节因子激活剂;DAI

产品类型

 单抗

产品属性

克隆号

 Zippy-1

亚型

 小鼠 IgG2a

来源/宿主

 从浓缩杂交瘤组织培养上清液中提取

免疫原/抗原

 重组小鼠ZBP1(aa 1-411)

应用

 IHC:(1:500)(请见参考文献1)
 IP:(1:200)
 WB:(1:1,000)

交叉反应性

 人/小鼠

特异性

 识别人和小鼠ZBP1

纯度

 ≥95%(SDS-PAGE)

纯化细节

 G蛋白亲和纯化

浓度

 1 mg/mL

配制

 液体,溶解在含10%甘油和0.02%叠氮化钠的PBS缓冲液中。

运输及处理

运输条件

 冰袋

短期储存

 +4℃

长期储存

 -20℃

处理建议

 打开后,等分处理并储存于-20℃条件下。避免反复冻融。

使用/稳定性

 收货后保存于-20℃条件下,可至少保存1年。

※ 本页面产品仅供研究用,研究以外不可使用。

参考文献



1.

ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol. 1, aag2045 (2016)


2.

DAI senses Influenza A virus genomic RNA and activates RIPK3-dependent cell death: R.J. Thapa, et al.; Cell Host & Microbe 20, 674 (2016)


3.

RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation: J. Lin, et al.; Nature 540, 124 (2016)


4.

RIPK1 inhibits ZBP1-driven necroptosis during development: K. Newton, et al.; Nature 540, 129 (2016)


5.

IRF1 Is a Transcriptional Regulator of ZBP1 Promoting NLRP3 Inflammasome Activation and Cell Death during Influenza Virus Infection: T. Kuriakose, et al.; J. Immunol. 200, 1489 (2018)


6.

Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1: H. Guo, et al.; Cell Death Dis. 9, 816 (2018) [KO Validation]


7.

Constitutive interferon signaling maintains critical threshold of MLKL expression to license necroptosis: J. Sarhan, et al.; Cell Death Diff. 26, 332 (2019)


产品编号 产品名称 产品规格 产品等级
AG-20B-0010-C100 anti-ZBP1, mAb (Zippy-1)
ZBP-1,单抗(Zippy-1)
100 μg

MP 116570200 FastDNA Spin Kit For 粪便

上海金畔生物科技有限公司提供MP 116570200 FastDNA Spin Kit For 粪便

品牌:MP
货号:116570200
中文名:FastDNA Spin Kit For 粪便
英文名:FastDNA Spin Kit For Feces
CAS号:
纯度:
包装:1 kit 50preps
价格:2399

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​anti-Asc, pAb (AL177) 凋亡相关斑点样蛋白 Asc 检测及功能抗体

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​anti-Asc, pAb (AL177)                              凋亡相关斑点样蛋白 Asc 检测及功能抗体anti-Asc,pAb (AL177)

凋亡相关斑点样蛋白 Asc 检测及功能抗体


Asc 促进半胱天冬酶介导的细胞凋亡。这种促凋亡活性主要通过半胱天冬酶-9 的活性介导。它是炎症小体的一个组成部分,炎症小体是包含 NLRP3/NALP3、CARD8 和 CASP1 的蛋白复合体,其功能是激活促炎性半胱氨酸蛋白酶。


产品详情

别名

 Pycard、甲基化诱导沉默的靶点1、TMS1、Caspase Recruitment Domain-containing Protein 5、

 细胞凋亡相关Speck样蛋白 CARD、CARD5

产品类型

 多克隆抗体

免疫动物/宿主

 兔

免疫原/抗原

 对应于 N 末端人 Asc 的氨基酸合成肽.

应用

 免疫细胞化学(1:200)
 免疫组织化学(1:500;paraffin sections)
 免疫沉淀(1:200)
 Western Blot(1:1000)
 功能应用(见参考文献 [1])

交叉反应性

 人、小鼠

特异性

 识别人和小鼠 Asc.

纯度

 表位亲和纯化

浓度

 1 mg/mL

配方

 液体,溶解在含有 10% 甘油和 0.02% 叠氮钠的 PBS 中

运输

 干冰

短时间储存

 +4°C

长时间储存

 -20°C

参考文献

[1]

NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini,et al.; Immunity 20, 319 (2004)


[2]

P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1beta in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180, 7147 (2008)


[3]

Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF: L. Kolly, et al.; J. Immunol. 183, 4003 (2009)


[4]

Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinatedinflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255  (2012)


[5]

NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.;  Nature 493, 674 (2013)


[6]

Promyelocytic leukemia protein interacts with the apoptosis-associated speck-like protein to limit inflammasome activation: J.K. Dowling, et al.; J. Biol. Chem. 289, 6429 (2014)


[7]

Localization and functionality of the inflammasome in neutrophils: M. Bakele, et al.; J. Biol. Chem. 289, 5320 (2014)


[8]

The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014)


[9]

The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)


[10]

Aging-associated TNF production primes inflammasome activation and Nlrp3-related metabolic disturbances: F. Bauernfeind, et al.; J. Immunol. 197, 2900 (2016)


[11]

An Investigation of Inflammatory and Metabolic Blood-Based Biomarkers of Cognitive Decline: H. Wolfe; Thesis Univ. Dublin, Trinity College Dep. of Physiol. (2018)


 

产品编号 产品名称 产品规格 产品等级
AG-25B-0006-C100 anti-Asc, pAb (AL177)
 Asc多抗(AL177)
100 μg

mR1ECM (modified Rat 1-cell Embryo Culture Medium) mR1ECM大鼠体外培养基 品牌:Cosmo Bio


品牌:Cosmo Bio
CAS No.:
储存条件:+4℃ DNF
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CSR-R-M174

10 x 5 ml 咨询


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