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Isoamylase (Glycogen 6-glucanohydrolase) 
Product code: E-ISAMY
€208.00
600 Units
Prices exclude VAT
| Content: | 600 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Isoamylase |
| EC Number: | 3.2.1.68 |
| CAZy Family: | GH13 |
| CAS Number: | 9067-73-6 |
| Synonyms: | isoamylase; glycogen 6-alpha-D-glucanohydrolase |
| Source: | Pseudomonas sp. |
| Molecular Weight: | 71,500 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Purified from Pseudomonas sp. |
| Specificity: | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
| Specific Activity: | ~ 180 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg) |
| Unit Definition: | One unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC. |
| Temperature Optima: | 50oC |
| pH Optima: | 4 |
| Application examples: | Applications in carbohydrate research and in the food and feeds, and cereals industry. |
| Method recognition: | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Please refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.
We have a wide range of other CAZyme products available.
Validation of Methods
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E-ISAMYHP – Isoamylase HP (Glycogen 6-glucanohydrolase)
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O-IPAN – Isopanose O-GMT – 63-α-D-Glucosyl-maltotriose O-PAN-1G – Panose P-BLDX-10G – β-Limit Dextrin P-GLYAL – Glycogen (Algae)
Associated Enzymes
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-BARBL-50KU – β-Amylase (Barley) E-PULKP – Pullulanase M1 (Klebsiella planticola) E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
Related Products
K-TDFR-200A – Total Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-TSTA-100A – Total Starch Assay Kit (AA/AMG) K-AMYL – Amylose/Amylopectin Assay Kit
Similar Products
E-ISAMYHP – Isoamylase HP (Glycogen 6-glucanohydrolase)
Related Carbohydrates
O-IPAN – Isopanose O-GMT – 63-α-D-Glucosyl-maltotriose O-PAN-1G – Panose P-BLDX-10G – β-Limit Dextrin P-GLYAL – Glycogen (Algae)
Associated Enzymes
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-BARBL-50KU – β-Amylase (Barley) E-PULKP – Pullulanase M1 (Klebsiella planticola) E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
Related Products
K-TDFR-200A – Total Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-TSTA-100A – Total Starch Assay Kit (AA/AMG) K-AMYL – Amylose/Amylopectin Assay Kit
