产品货号：ZZK-K-ETSULPH

产品品名：二氧化硫检测试剂盒

英文品名：Total Sulphite (Enzymatic) Assay Kit

规格型号：50 assays (manual) / 500 assays (microplate) / 588 assays (auto-analyser)

A simple, highly specific and reliable, enzymatic method for themeasurement of total sulphite (as SO2) in wines, beverages, foodstuffsand other materials. Suitable for manual, auto-analyser and microplate formats.

INTRODUCTION:

Sulphur dioxide (SO2) is used widely as an additive in various forms, most

commonly as sulphites (or sulfites), in the wine, beverage and food industries

where it acts primarily as an antimicrobial and antioxidant preservative.

During wine making, sulphites are used as an essential additive, usually

at post-malolactic fermentation, in the control of contamination by

Brettanomyces during ageing and to also protect the wine against

detrimental “oxidative and enzymatic browning”. SO2 is only active

as an antimicrobial and antioxidant preservative in the unbound

“free” form. Given that SO2 becomes “inactive” when it binds the

colour pigments of wine, and with legal restrictions on SO2 levels in

wine, it has become valuable to wine producers to measure both the

Free SO2 and Total SO2. In addition to this, due to the increased

awareness of the adverse effects of sulphites and the prevalence

of sulphite intolerance in some individuals, sulphite levels in foods

and drinks are strictly regulated by various governing bodies and

therefore there is a requirement for accurate determination of the

level of sulphites in foods, beverages and wines.

This kit (K-ETSULPH) is suitable for the specific measurement of “Total

Sulphite” as SO2 in wines, beverages, foodstuffs and other materials.

KITS:

Kits suitable for performing 50 assays in manual format (or 588

assays in auto-analyser format or 500 assays in microplate format) are

available from Megazyme. The kits contain the full assay method plus:

Bottle 1: Buffer (28 mL; pH 8.0) plus sodium azide

(0.02% w/v) as a preservative.

Stable for 大于 2 years at 4°C.

Bottle 2: NADH.

Freeze dried powder.

Stable for 大于 2 years at -20°C.

Bottle 3: NADH peroxidase suspension (1.1 mL).

Stable for 大于 2 years at 4°C.

Bottle 4: Sulfite oxidase suspension (1.1 mL).

Stable for 大于 2 years at 4°C.

Bottle 5: Sodium sulphite standard powder (~ 5 g).

Stable for 大于 5 years at room temperature.

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