lumiprobe荧光染料Cyanine3 azide

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Cyanine3 azide
Cyanine3 azide

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Cyanine3 azide

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Items in stock will be shipped on 04/22/2019
Cat. # Quantity Price Lead time Buy this product
11030 100 uL, 10 mM/DMSO $110.00 Cyanine3 azide in stock

31030 500 uL, 10 mM/DMSO $210.00 Cyanine3 azide in stock

41030 1 mL, 10 mM/DMSO $410.00 Cyanine3 azide in stock

A1030 1 mg $110.00 Cyanine3 azide in stock

B1030 5 mg $210.00 Cyanine3 azide in stock

C1030 10 mg $310.00 Cyanine3 azide in stock

D1030 25 mg $410.00 Cyanine3 azide in stock

E1030 50 mg $695.00 Cyanine3 azide in stock

F1030 100 mg $1190.00 Cyanine3 azide in stock

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Cyanine3 azide

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Cyanine3 azide

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Cyanine3 dye azide for Click Chemistry, an analog of Cy3® azide. Cy3® is one of the most broadly used fluorophores which can be detected by various fluorometers, imagers, and microscopes. Due to inherently high extinction coefficient, Cyanine3 is also easily detected by naked eye on gels, and in solution. This is non-sulfonated dye which requires organic co-solvent (DMF, DMSO, or other) for efficient labeling in water. Water-soluble version of this reagent is also available.

Product is available both as solid compound, and as 10 mM solution in DMSO which is ready to use in our recommended protocol.

Cyanine3 fluorescent properties are identical to Cy3®, and similar to Alexa Fluor 546, and DyLight 549.

Cyanine3 azide
Cyanine3 azide
Cyanine3 azide
Cyanine3 azide
Cyanine3 azide
Cyanine3 azide
Cyanine3 azide
Cyanine3 azide

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Cy3 absorbance and emission spectra

Cyanine3 azide

Recommended protocol

Click-Chemistry Labeling of Oligonucleotides and DNACyanine3 azide

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Sulfo-Cyanine5 NHS ester

Water-soluble Cyanine 5 succinimide ester.

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Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine7 NHS ester is a water soluble, near infrared, amine-reactive dye.

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General properties

Appearance: red powder / solution
Molecular weight: 575.19
CAS number: 1167421-28-4
Molecular formula: C33H43N6OCl
Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (40 mg/L = 60 uM)
Quality control: NMR 1H and HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: Download
Product specifications

Spectral properties

Excitation maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

Product citations

  1. Kasprzyk, R.; Starek, B.J.; Ciechanowicz, S.; Kubacka, D.; Kowalska, J.; Jemielity, J. Fluorescent turn-on probes for the development of binding and hydrolytic activity assays for mRNA cap-recognising proteins. Chemistry, in press. doi: 10.1002/chem.201900051
  2. Kumar, R.; Welle, A.; Becker, F.; Kopyeva, I.; Lahann, J. Substrate-Independent Micropatterning of Polymer Brushes Based on Photolytic Deactivation of Chemical Vapor Deposition Based Surface-Initiated Atom-Transfer Radical Polymerization Initiator Films. Applied Materials & Interfaces, 2018, 10(38), 31965–31976. doi: 10.1021/acsami.8b11525
  3. Navarro, L.A.; French, D.L.; Zauscher, S. Synthesis of Modular Brush Polymer-Protein Hybrids using Diazotransfer and Copper Click Chemistry. Bioconjugate Chemistry, 2018, 29(8), 2594–2605. doi: 10.1021/acs.bioconjchem.8b00309
  4. Tsai, C.-Y.; Li, C.-W.; Li, J.-R.; Jang, B.-H.; Chen, S.-H. Steroid Probes Conjugated with Protein-Protected Gold Nanocluster: Specific and Rapid Fluorescence Imaging of Steroid Receptors in Target Cells. Journal of Fluorescence, 2016, 26(4), 1239–1248. doi: 10.1007/s10895-016-1811-6

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