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AF430 is a dye that is structurally identical to Alexa Fluor® 430. The fluorophore is hydrophilic. It is used in flow cytometry and other applications.
The NHS ester functional group allows to conjugate the fluorophore efficiently with primary and secondary amine groups in both aqueous buffers and anhydrous conditions.
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Absorption and emission spectra of AF430
Recommended protocol
NHS ester labeling of amino biomolecules
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Protein labeling calculator
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TAMRA NHS ester, 5-isomer
Amine-reactive, activated N-hydroxysuccinimidyl ester of TAMRA dye for the labeling of amine groups of proteins, peptides, and amino-oligonucleotides.
