lumiprobe荧光染料DusQ2 CPG 500

发表于2024年1月20日由上海金畔生物科技有限公司

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DNA
CPG solid supports
DusQ2 CPG 500

DusQ2 CPG 500

CPG (controlled pore glass) solid supports for oligonucleotide synthesis

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Items in stock will be shipped on 13.12.2021

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Cat. #	Quantity	Price
12960	100 mg	– Sample request
32960	500 mg	530.00$
42960	1 g	990.00$
52960	5 g	please inquire Request

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This support is intended for automated synthesis of oligonucleotides with 3’-terminal DusQ2 quencher. Pore size of 500 Å is recommended for the synthesis of oligonucleotides of up to 50 bases in length.

DusQ2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar^® 570, Pulsar^® 650, Cyanine5, Quasar 670, Cyanine5.5, and Quasar 705.

Usage

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.