CPG (controlled pore glass) solid supports for oligonucleotide synthesis
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15960
100 mg
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35960
500 mg
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45960
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55960
5 g
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Items in stock will be shipped on 13.12.2021
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This modified support is intended for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides of up to 120 bases in length.
DusQ2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend very much on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Quasar® 570, Pulsar® 650, Cyanine5, Quasar 670, Cyanine5.5, and Quasar 705.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
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Absorption spectrum of DusQ2
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Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ2 quencher at the 3’ end. Compatible with standard deblocking conditions.
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