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6-fluorescein (FAM) derivative of uridine triphosphate (UTP). FAM is a fluorophore with a high quantum yield. The maximum emission of the fluorescence is at a wavelength of 513 nm in the green spectrum range.
6-FAM-11-UTP can be used as a substrate for RNA-polymerases T7, T3 and SP6 during in vitro transcription. RNA-probes produced with this method can be used for fluorescence hybridization in situ, including multiplex, and for Northern blot. Fluorescently-labeled cRNA can be used for microarray-based gene expression profiling.
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Absorption and emission spectra of FAM
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Amino-11-UTP
Uridine triphosphate labeled with amino group intended for indirect non-radioactive enzymatic RNA labeling.