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EdU (ethynyldeoxyuridine) is a nucleoside, an analog of thymidine, carrying an ethynyl group. This nucleoside behaves as a substrate for cellular DNA replication machinery. Then, DNA containing ethynyl groups can be developed by Click chemistry reaction with various dye azides. Fluorescent DNA can be detected by microscopy, or cells can be sorted by FACS.
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Assaying cell proliferation and DNA replication with Click Chemistry