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Dibenzocyclooctyne (ADIBO, DBCO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC) – a copper free Click chemistry reaction.
This is an amine-reactive NHS ester that provides easy attachment of the reactive moiety to almost any primary or secondary amine group, such as that of protein, peptide, or small molecule amine.
DBCO reacts instantly with azides. The rate of the reaction is much higher than that of copper-catalyzed reaction, and reactions with many other cyclooctynes. Unlike some other cyclooctynes, DBCO does not react with tetrazines – this allows to carry out orthogonal conjugation of azides with DBCO, and trans-cyclooctenes with tetrazines.
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BDP 630/650 is a far red emitting fluorophore for Cyanine5 channel. This fluorophore is based on BDP (borondipyrromethene) core, which provides high quantum yield and photostability. This is an amine derivative of this dye.