Micro-Bridges蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

Micro-Bridges
Micro-Bridges

Micro-Bridges

Micro-Bridges

Micro-Bridges

Micro-Bridges

Products > Crystallization Plates, Hardware & Accessories > Micro-Bridges > Micro-Bridges

Micro-Bridges

Applications

  • Sitting drop crystallization
  • Heavy atom soaks
  • Seeding

Features

  • Perform sitting drop experiments in 24 well hanging drop plates
  • Removable

Description

Micro-Bridges are small devices in the shape of a bridge that are designed to carry out sitting drop vapor diffusion crystallization when placed in a VDX™ Plate or Linbro® Plate. A single Micro-Bridge fits neatly into the reservoir of a standard 24 well VDX Plate for a sitting drop crystallization experiment. Once placed inside the wells, Micro-Bridges are stable and there is no need to stick them to the wells with grease or adhesive. It is therefore possible to transfer them to other wells during or after a crystallization experiment.

Why sitting drop? Placing the droplet in the indentation greatly reduces the risk of losing the protein by accident. Crystallization can be carried out in the presence of detergents and organic solvents which are compatible with polystyrene (such as MPD, iso-propanol, and ethanol). The protein drop is less affected by condensation problems. Soaking and seeding experiments can be carried out easily. Crystals can be transported more securely. Larger drop volumes can be used. Micro-Bridges have a concave indentation in the top surface of the bridge which holds the sample droplet during a crystallization experiment and prevents the droplet from spreading over a large area. Made from polystyrene, these parts are highly transparent and suitable for most crystallizations. The surface of the indentation is highly polished to facilitate the visual inspection of the drops under a microscope. The maximum drop volume for the Micro-Bridge is 35 µl. Reservoirs can be sealed with plain cover slides and vacuum grease or clear sealing tape.

Approximate dimensions: Height: 12 mm Length: 14 mm Width: 7 mm

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Micro-Bridges

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Micro-Bridges
Micro-Bridges
Micro-Bridges
Micro-Bridges
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Micro-Bridges

Micro-Bridges

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Micro-Bridges
Micro-Bridges
Micro-Bridges
Micro-Bridges
Micro-Bridges
Micro-Bridges

CAT NO

HR3-310

NAME

Micro-Bridges

DESCRIPTION

100 pack

PRICE

$81.00

cart quote

CAT NO

HR3-312

NAME

Micro-Bridges

DESCRIPTION

400 pack

PRICE

$282.00

cart quote

Support Material(s)

Micro-Bridges HR3-310 HR3-312 Micro-Bridges User Guide

References

1. Structure of aluminum-bound ovotransferrin at 2.15 angstrm resolution. K, Mizutani, B. Mikami, S. Aibara, and M. Hirose. Acta Cryst (2005) D61, 1636-1642.

Micro-Bridges Micro-Bridges

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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