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Products > Crystallization Plates, Hardware & Accessories > Dialysis Buttons, Membranes & Applicators > Dialysis Buttons
Dialysis Buttons
Applications
- Crystallization by dialysis, protein folding, & small volume sample dialysis
Features
- Low volume dialysis
- Fits in 24 well plates
- Use over and over again
Description
In the dialysis method, the sample in question is separated from the “precipitant” by a semi-permeable membrane which allows small reagent molecules to pass but prevents biological macromolecules from crossing the membrane.1-3
Dialysis Buttons are injection molded from polystyrene, and are the size of a small button. The sample is placed in this chamber so as to create a slight dome of liquid at the top of the button. A dialysis membrane is placed over the top of the button/sample and is held in place with an O-ring. The O-ring is held in place by a groove in the Dialysis Button. Dialysis Buttons are supplied with O-rings. The 5 µl to 100 µl sized dialysis buttons have an approximate overall height of 6 mm, an approximate outside diameter of 10 mm without an O-ring in place, and an approximate diameter of 12 mm with an O-ring in place. The 200 µl and 350 µl sized dialysis buttons have an approximate overall height of 8 mm, an approximate outside diameter of 15 mm without an O-ring in place, and an approximate diameter of 17 mm with an O-ring in place. Each package of dialysis buttons includes a plastic golf tee shaped applicator to assist with applying a dialysis membrane to the button and securing with the supplied o-ring.
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CAT NO
HR3-336
NAME
DESCRIPTION
5 of each size listed below
PRICE
$100.00
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CAT NO
HR3-314
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-316
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-318
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-320
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-326
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-328
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-330
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
CAT NO
HR3-332
NAME
DESCRIPTION
50 pack
PRICE
$100.00
cart quote
Support Material(s)
Dialysis Button User Guide Related Item(S)
- Dialysis Membrane Discs for Buttons
References
1. McPherson, A., Preparation and Analysis of Protein Crystals, Krieger Publishing, 88-91 (1992).
2. Durcruix, A, and Giege, R., Crystallization of Nucleic Acids and Proteins, A Practical Approach, Oxford University Press, 78-82 (1992).
3. Zeppenzauer, M., et al., Acta Chem Scan (1967) 21, 1009.
4. Structure of the membrane domain of respiratory complex I. Efremov RG, Sazanov LA. Nature. 2011 Aug 7;476(7361):414-20. doi: 10.1038/nature10330.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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