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Pectate Lyase (Aspergillus sp.)
Product code: E-PCLYAN
€142.00
7,000 Units in 50% aqueous glycerol
Prices exclude VAT
| Content: | 7,000 Units in 50% aqueous glycerol |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 4 years at 4oC or below -10oC |
| Enzyme Activity: | Pectate Lyase |
| EC Number: | 4.2.2.2 |
| CAZy Family: | PL1 |
| CAS Number: | 9015-75-2 |
| Synonyms: | pectate lyase; (1→4)-alpha-D-galacturonan lyase |
| Source: | Aspergillus sp. |
| Molecular Weight: | 45,000 |
| Concentration: | Supplied at ~ 1,400 U/mL |
| Expression: | Purified from Aspergillus sp. |
| Specificity: | Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends. |
| Specific Activity: | ~ 180 U/mg (40oC, pH 8.0 on polygalacturonic acid) |
| Unit Definition: | One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40oC. |
| Temperature Optima: | 55oC |
| pH Optima: | 8 |
| Application examples: | Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research. |
High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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E-PCLYAN2 – Pectate Lyase (Aspergillus sp.) E-PLYCJ – Pectate lyase (Cellvibrio japonicus)
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P-PGAUP – Polygalacturonic Acid (ultrapure) P-PGACIT-10G – Polygalacturonic Acid (from Citrus Pectin)