Magazyme试剂盒Isoamylase Flavobacterium odoratum E-ISAMYFO

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Isoamylase (Glycogen 6-glucanohydrolase) 
(Flavobacterium odoratum) Isoamylase Flavobacterium odoratum E-ISAMYFO Isoamylase Flavobacterium odoratum E-ISAMYFO

Product code: E-ISAMYFO-200U

Content:

€100.00

200 Units

Prices exclude VAT

  • Data Sheet
  • SDS
  • COAs
  • Content: 200 Units or 800 Units
    Shipping Temperature: Ambient
    Storage Temperature: 4oC
    Formulation: In 3.2 M ammonium sulphate
    Physical Form: Suspension
    Stability: > 4 years at 4oC
    Enzyme Activity: Isoamylase
    EC Number: 3.2.1.68
    CAZy Family: GH13
    CAS Number: 9067-73-6
    Synonyms: isoamylase; glycogen 6-alpha-D-glucanohydrolase
    Source: Flavobacterium odoratum
    Molecular Weight: 83,000
    Concentration: Supplied at ~ 200 U/mL
    Expression: Purified from Flavobacterium odoratum
    Specificity: Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
    Specific Activity: ~ 140 U/mg (40oC, pH 6.0 with 1 mM CaClon oyster glycogen)
    Unit Definition: One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100mM, pH 6.0) plus 1 mM CaCl2 at 40oC.
    Temperature Optima: 50oC
    pH Optima: 6
    Application examples: Applications in carbohydrate research and in the food and feeds, and cereals industry.
    Method recognition: AOAC Method 2000.11 and GB Standard 5009.245-2016

    Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

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    Related Carbohydrates
    O-IPAN – Isopanose O-GMT – 63-α-D-Glucosyl-maltotriose O-PAN-1G – Panose P-BLDX-10G – β-Limit Dextrin P-GLYAL – Glycogen (Algae)

    Associated Enzymes
    E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-BARBL-50KU – β-Amylase (Barley) E-PULKP – Pullulanase M1 (Klebsiella planticola) E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)

    Related Products
    K-TDFR-200A – Total Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-TSTA-100A – Total Starch Assay Kit (AA/AMG) K-AMYL – Amylose/Amylopectin Assay Kit

    Similar Products
    E-ISAMY – Isoamylase (Glycogen 6-glucanohydrolase) E-ISAMYHP – Isoamylase HP (Glycogen 6-glucanohydrolase)
    Related Carbohydrates
    O-IPAN – Isopanose O-GMT – 63-α-D-Glucosyl-maltotriose O-PAN-1G – Panose P-BLDX-10G – β-Limit Dextrin P-GLYAL – Glycogen (Algae)
    Associated Enzymes
    E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-BARBL-50KU – β-Amylase (Barley) E-PULKP – Pullulanase M1 (Klebsiella planticola) E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
    Related Products
    K-TDFR-200A – Total Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-TSTA-100A – Total Starch Assay Kit (AA/AMG) K-AMYL – Amylose/Amylopectin Assay Kit

    Isoamylase Flavobacterium odoratum E-ISAMYFO