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3-Hydroxybutyrate dehydrogenase (prokaryote) 
Product code: E-HBDH
€173.00
200 Units
Prices exclude VAT
| Content: | 200 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.30 |
| CAS Number: | 9028-38-0 |
| Synonyms: | 3-hydroxybutyrate dehydrogenase; (R)-3-hydroxybutanoate:NAD+ oxidoreductase |
| Source: | Prokaryote |
| Molecular Weight: | 29,177 |
| Concentration: | Supplied at ~ 100 U/mL |
| Expression: | Recombinant from a Prokaryotic source |
| Specificity: | Catalyses the reaction: D-3-Hydroxybutyrate + NAD+ = acetoacetate + NADH + H+ |
| Specific Activity: | ~ 77.4 U/mg (25oC, pH 8.0 on D-β-hydroxybutyric acid) |
| Unit Definition: | One Unit of 3-hydroxybutyrate dehydrogenase is defined as the amount of enzyme required to oxidise one µmole of D-β-hydroxybutyric acid (28.3 mM) per minute in the presence of NAD+ in Tris-HCl buffer (143 mM), pH 8.0 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8 |
| Application examples: | Applications for the measurement of D-3-Hydroxybutyrate in the food, fermentation, wine, beverage and dairy industries. |
High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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Associated Enzymes
E-FDHCB – Formate dehydrogenase (Candida boidinii) E-GALDH – Galactose dehydrogenase (soil prokaryote) E-GALMUT – Galactose dehydrogenase – Galactose mutarotase E-GPDH5 – Glucose-6-phosphate dehydrogenase (Leuconostoc mesenteroides) E-GPDHEC – Glucose-6-phosphate dehydrogenase
(Escherichia coli) E-HKGDH – Hexokinase/Glucose-6-phosphate dehydrogenase E-INDHBS – myo-Inositol dehydrogenase (Bacillus subtilis) E-DLDHLM – D-Lactate dehydrogenase
(Leuconostoc mesenteroides) E-LLDHP – L-Lactate dehydrogenase (Porcine) E-LMDHEC – L-Malate dehydrogenase (Escherichia coli) E-MNHPF – Mannitol dehydrogenase
(Pseudomona fluorescens) E-PGDHEC – 6-Phosphogluconate dehydrogenase
(Escherichia coli) E-XYLMUT – Xylose dehydrogenase + Xylose mutarotase
(Escherichia coli) E-HKGDH – Hexokinase/Glucose-6-phosphate dehydrogenase E-INDHBS – myo-Inositol dehydrogenase (Bacillus subtilis) E-DLDHLM – D-Lactate dehydrogenase
(Leuconostoc mesenteroides) E-LLDHP – L-Lactate dehydrogenase (Porcine) E-LMDHEC – L-Malate dehydrogenase (Escherichia coli) E-MNHPF – Mannitol dehydrogenase
(Pseudomona fluorescens) E-PGDHEC – 6-Phosphogluconate dehydrogenase
(Escherichia coli) E-XYLMUT – Xylose dehydrogenase + Xylose mutarotase
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C-NAD-5G – β-Nicotinamide Adenine Dinucleotide (NAD+), Oxidised C-NADP-2G – β-Nicotinamide Adenine Dinucleotide Phosphate (NADP+), Oxidised Sodium Salt C-NADH-2G – β-Nicotinamide Adenine Dinucleotide (NADH), Reduced Disodium Salt
Associated Enzymes
E-FDHCB – Formate dehydrogenase (Candida boidinii) E-GALDH – Galactose dehydrogenase (soil prokaryote) E-GALMUT – Galactose dehydrogenase – Galactose mutarotase E-GPDH5 – Glucose-6-phosphate dehydrogenase (Leuconostoc mesenteroides) E-GPDHEC – Glucose-6-phosphate dehydrogenase
(Escherichia coli) E-HKGDH – Hexokinase/Glucose-6-phosphate dehydrogenase E-INDHBS – myo-Inositol dehydrogenase (Bacillus subtilis) E-DLDHLM – D-Lactate dehydrogenase
(Leuconostoc mesenteroides) E-LLDHP – L-Lactate dehydrogenase (Porcine) E-LMDHEC – L-Malate dehydrogenase (Escherichia coli) E-MNHPF – Mannitol dehydrogenase
(Pseudomona fluorescens) E-PGDHEC – 6-Phosphogluconate dehydrogenase
(Escherichia coli) E-XYLMUT – Xylose dehydrogenase + Xylose mutarotase
(Escherichia coli) E-HKGDH – Hexokinase/Glucose-6-phosphate dehydrogenase E-INDHBS – myo-Inositol dehydrogenase (Bacillus subtilis) E-DLDHLM – D-Lactate dehydrogenase
(Leuconostoc mesenteroides) E-LLDHP – L-Lactate dehydrogenase (Porcine) E-LMDHEC – L-Malate dehydrogenase (Escherichia coli) E-MNHPF – Mannitol dehydrogenase
(Pseudomona fluorescens) E-PGDHEC – 6-Phosphogluconate dehydrogenase
(Escherichia coli) E-XYLMUT – Xylose dehydrogenase + Xylose mutarotase
Related Products
C-NAD-5G – β-Nicotinamide Adenine Dinucleotide (NAD+), Oxidised C-NADP-2G – β-Nicotinamide Adenine Dinucleotide Phosphate (NADP+), Oxidised Sodium Salt C-NADH-2G – β-Nicotinamide Adenine Dinucleotide (NADH), Reduced Disodium Salt
