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Glucuronoyl Esterase (Ruminococcus flavefaciens) 
Product code: E-GERF
€156.00
250 Units
Prices exclude VAT
| Content: | 250 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Esterase |
| EC Number: | 3.1.1.B11 |
| CAZy Family: | CE15 |
| CAS Number: | 9016-18-6 |
| Synonyms: | glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase |
| Source: | Ruminococcus flavefaciens |
| Molecular Weight: | 47,000 |
| Concentration: | Supplied at ~ 370 U/mL |
| Expression: | Recombinant from Ruminococcus flavefaciens |
| Specificity: | Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. |
| Specific Activity: | ~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3) |
| Unit Definition: | One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3. |
| Temperature Optima: | 40oC |
| pH Optima: | 7 |
| Application examples: | Applications in carbohydrate research and in the food industry. |
High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
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