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Galactose dehydrogenase – Galactose mutarotase 
1 mL; galactose dehydrogenase (200 U/mL) /
galactose mutarotase (4 mg/mL)
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| Content: | 1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarotase (4 mg/mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | Galactose dehydrogenase: 1.1.1.48 Galactose mutarotase: 5.1.3.3 |
| CAS Number: | Galactose dehydrogenase: 9028-54-0 Galactose mutarotase: 9031-76-9 |
| Synonyms: | Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase |
| Source: | Escherichia coli |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1).
(1) α-D-Galactose ↔ β-D-Galactose. The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2). (2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+ |
| Specific Activity: | Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC Galactose mutarotase: 4.1 mg/mL |
| Unit Definition: | One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8.6, at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8.6 |
| Application examples: | Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via D-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR). |
High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.
For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)
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