Magazyme试剂盒Fructanase Mixture Ultrapure recombinant powder E-FRPDPU

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Fructanase Mixture
(Ultrapure, recombinant, powder) Fructanase Mixture Ultrapure recombinant powder E-FRPDPU Fructanase Mixture Ultrapure recombinant powder E-FRPDPU

Product code: E-FRPDPU

€260.00

20,000 Units

Prices exclude VAT

  • Data Sheet
  • SDS
  • COAs
  • Presentations
  • Content: 20,000 Units
    Shipping Temperature: Ambient
    Storage Temperature: Below -10oC
    Formulation: Supplied as a freeze-dried powder
    Physical Form: Powder
    Stability: Minimum 1 year at < -10oC. Check vial for details.
    Enzyme Activity: Fructanase
    EC Number: endo-Inulinase: 3.2.1.7,
    exo-Inulinase: 3.2.1.80
    CAZy Family: GH32
    CAS Number: 9001-57-4,
    9025-67-6,
    37288-56-5
    Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
    exo-inulinase: fructan β-fructosidase
    Source: Aspergillus niger
    Concentration: endo-inulinase: Supplied at ~ 100 U/mL
    exo-inulinase: Supplied at ~ 2,000 U/mL
    Expression: Recombinant from Aspergillus niger
    Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
    exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
    Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
    exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
    Temperature Optima: 60oC
    pH Optima: 4.5

    Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

    Componentsexo-Inulinase 20,000 U (on kestose at 40oC), endo-inulinase 1000 U (on fructan at 40oC).

    Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

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    Fructanase Mixture Ultrapure recombinant powder E-FRPDPU

    Fructanase Mixture Ultrapure recombinant powder E-FRPDPU