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Amyloglucosidase (Aspergillus niger) 
40 mL
Prices exclude VAT
| Content: | 10 mL or 40 mL or 100 mL or 100 mL (ANKOM) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Amyloglucosidase |
| EC Number: | 3.2.1.3 |
| CAZy Family: | GH15 |
| CAS Number: | 9032-08-0 |
| Synonyms: | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase |
| Source: | Aspergillus niger |
| Molecular Weight: | 143,500 |
| Expression: | From Aspergillus niger |
| Specificity: | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. |
| Specific Activity: | ~ 36 U/mg (40oC, pH 4.5 on soluble starch); ~ 200 U/mL (40oC, pH 4.5 on p-nitrophenyl β-maltoside); ~ 3,260 U/mL (40oC, pH 4.5 on soluble starch) |
| Unit Definition: | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC. |
| Temperature Optima: | 70oC |
| pH Optima: | 4 |
| Application examples: | Applications for the measurement of starch and dietary fiber in the food and feeds industry. |
| Method recognition: | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Total Starch and Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
Data booklets for each pack size are located in the Documents tab.
We offer a wide array of Carbohydrate Active enZYme products for various uses.
Validation of Methods
Glycerol Free E-AMGDFPD – Amyloglucosidase (Aspergillus niger) Powder E-AMGFR-100MG – Amyloglucosidase (Aspergillus niger) E-AMGPU – Amyloglucosidase (Rhizopus sp.) E-GAMP – Glucoamylase P (H. resinae)
Glycerol Free E-AMGDFPD – Amyloglucosidase (Aspergillus niger) Powder E-AMGFR-100MG – Amyloglucosidase (Aspergillus niger) E-AMGPU – Amyloglucosidase (Rhizopus sp.) E-GAMP – Glucoamylase P (H. resinae)
