PEG/Ion • PEG/Ion 2 • PEG/Ion HT蛋白结晶试剂盒Hampton Research

Hampton Research蛋白结晶试剂盒

PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Products > Crystallization Screens > PEG/Ion • PEG/Ion 2 > PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Applications

  • Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules

Features

  • Developed at Hampton Research
  • PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2
  • PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350
  • PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block

Description

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules.

The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

PEG/Ion contains 48 unique reagents, 10 ml each.

PEG/Ion 2 contains 48 unique reagents, 10 ml each.

PEG/Ion HT contains 1 ml of each reagent from PEG/Ion and PEG/Ion 2 in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

PEG/Ion • PEG/Ion 2 • PEG/Ion HT

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PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT
PEG/Ion • PEG/Ion 2 • PEG/Ion HT

CAT NO

HR2-126

NAME

PEG/Ion Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-098

NAME

PEG/Ion 2 Screen

DESCRIPTION

10 ml, tube format

PRICE

$276.00

cart quote

CAT NO

HR2-139

NAME

PEG/Ion HT

DESCRIPTION

1 ml, Deep Well block format

PRICE

$161.00

cart quote

Support Material(s)

PEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-126 PEG/Ion DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-126 PEG/Ion SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-098 PEG/Ion 2 DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-098 PEG/Ion 2 SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-139 PEG/Ion HT DocumentsPEG/Ion • PEG/Ion 2 • PEG/Ion HT HR2-139 PEG/Ion HT SDSPEG/Ion • PEG/Ion 2 • PEG/Ion HT PEG/Ion Formulation & Scoring Data

Related Item(S)

  • Individual PEG/Ion • PEG/Ion 2 • PEG/Ion HT Reagents

References

1. Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase. C. V. dos Reis, A. Bernardes and I. Polikarpov. Acta Cryst. (2013). F69, 588-591.

2. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

3. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology Volume 156, Issue 3 , December 2006, Pages 387-406.

4. Mechanisms for Glycolipid Antigen-Driven Cytokine Polarization by Valpha14i NKT Cells. Sulliivan et al, The Journal of Immunology, 2010, 184, 141 -153. (PEG/Ion 2)

5. Optimization of salt concentration in PEG-based crystallization solutions. Yamanaka M, Inaka K, Furubayashi N, Matsushima M, Takahashi S, Tanaka H, Sano S, Sato M, Kobayashi T, Tanaka T. J Synchrotron Radiat. 2011 Jan;18(1):84-7. doi: 10.1107/S0909049510035995. Epub 2010 Nov 5.

6. Crystallization and preliminary X-ray crystallographic analysis of importin-[alpha] from Neurospora crassa. N. E. Bernardes, A. A. S. Takeda, F. Z. Freitas, M. C. Bertolini and M. R. M. Fontes. Acta Cryst. (2014). F70, 501-504 [ doi:10.1107/S2053230X14005068 ]

PEG/Ion • PEG/Ion 2 • PEG/Ion HT PEG/Ion • PEG/Ion 2 • PEG/Ion HT

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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