NGAL (human) ELISA kit 肾损伤标志物 NGAL 检测试剂盒

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NGAL (human) ELISA kitNGAL (human) ELISA kit                              肾损伤标志物 NGAL 检测试剂盒

肾损伤标志物 NGAL 检测试剂盒

急性肾损伤(Acute Kidney Injury,AKI)是一组临床综合征,以急性肾功能(Acute Renal Failure,ARF)减退为特征。针对 AKI 的诊断及研究,已证实存在于血液或尿液中的多种蛋白可成为肾损伤、肾疾病或肾毒性诊断及愈后的标志物。

其中,中性粒细胞明胶酶相关脂质运载蛋白(NGAL,lipocalin-2,siderocalin)是一种 25 kDa 的小蛋白,在全身上皮组织中表达。其可作为肾损伤标志物,当肾小管损伤后,NGAL 可从患者的尿液和血液中检出。

NGAL(人)ELISA 试剂盒,可在 450 nm 处高灵敏度检测人样本中 NGAL,低至 4 pg/mL。

 

特点

● 96 孔板检测,只需 4 小时即可快速检测最多 40 个样品及其平行组

● 高灵敏检测,可检测低至 4 pg/mL 的 NGAL

● 试剂盒包含即用型预涂板和独立标准溶液,可节省时间并减少操作失误

● 全定量分析,有别于半定量分析的免疫印迹法

◆NGAL(人)ELISA试剂盒(KIT 036)- 标准曲线

NGAL (human) ELISA kit                              肾损伤标志物 NGAL 检测试剂盒

◆产品详情

NGAL别称

中性粒细胞明胶酶相关脂质运载蛋白、脂质运载蛋白2、铁运载蛋白、癌基因产物24p3

灵敏度

4 pg/mL(范围10-1000 pg/mL)

检测时间

小于4小时

应用

ELISA、比色检测

应用说明

用于定量检测人尿液、血浆、血清、组织提取物和培养上清液中的NGAL

波长

450 nm

物种反应性

运输条件

非冷冻蓝冰

长期储存

+4°C

组分

1. 12 × 8 孔微孔板条,每条均涂有抗 NGAL抗体(共96孔)+板条框

2. 样品稀释液

3. NGAL标准溶液1-8:0,10,25,50,100,250,500,1000 pg/mL

4. 25X浓缩洗涤液

5. 生物素化 NGAL 抗体

6. HRP-链霉亲和素偶联物

7. TMB底物

8. 终止液

技术信息/产品说明

点击此处查看操作手册

UniProt ID

P80188

监管状态

RUO – 仅供研究用

制造商

BioPorto Diagnostics

◆产品列表

产品编号

产品名称

包装

BPD-KIT-036

NGAL (human) ELISA kit
NGAL(人)ELISA试剂盒

96 wells




◆其他物种的 NGAL 试剂盒

除NGAL (人) ELISA试剂盒外,另可提供小鼠、大鼠、猪、狗、猴的 NGAL ELISA 试剂盒。

NGAL (human) ELISA kit                              肾损伤标志物 NGAL 检测试剂盒

产品编号

产品名称

包装

BPD-KIT-042

NGAL (mouse) ELISA kit
NGAL(小鼠)ELISA试剂盒

96 wells

BPD-KIT-043

NGAL (dog) ELISA kit
NGAL(狗)ELISA试剂盒

96 wells

BPD-KIT-044

NGAL (pig) ELISA kit
NGAL(猪)ELISA试剂盒

96 wells

BPD-KIT-045

NGAL (monkey) ELISA kit
NGAL(猴)ELISA试剂盒

96 wells

BPD-KIT-046

NGAL (rat) ELISA kit
NGAL(大鼠)ELISA试剂盒

96 wells

 

参考文献

1.

The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males: T. Tominaga, et al.; Antioxidants (Basel) 10, 866 (2021), Application(s): NGAL quantification on human urine samples, 摘要全文


2.

Prognostic value of urinary calprotectin, NGAL and KIM-1 in chronic kidney disease: F.S. Seibert, et al.; Kidney Blood Press. Res. 43, 1255 (2018), Application(s): ELISA using human urine, 摘要;


3.

Acute kidney injury prediction in cardiac surgery patients by a urinary peptide pattern: a case-control validation study: J. Metzger, et al.; Crit. Care 20, 157 (2016), 摘要全文


4.

An AKI biomarker lipocalin 2 in the blood derives from the kidney in renal injury but from neutrophils in normal and infected conditions: J. Kanda, et al.; Clin. Exp. Nephrol. 19, 99 (2015), 摘要;


5.

Urinary neutrophil gelatinase-associated lipocalin predicts kidney outcome and death in patients with cirrhosis and bacterial infections: R. Barreto, et al.; J. Hepatol. 61, 35 (2014), Application(s): ELISA using human urine, 摘要;


6.

A comparison of the analytical performance of five commercially available assays for neutrophil gelatinase-associated lipocalin using urine: R.L. Kift, et al.; Ann. Clin. Biochem. 50, 236 (2013), 摘要;


7.

Albumin oxidation leads to neutrophil activation in vitro and inaccurate measurement of serum albumin in patients with diabetic nephropathy: R. Michelis, et al.; Free Radic. Biol. Med. 60, 49 (2013), 摘要;


8.

Association between plasma neutrophil gelatinase associated lipocalin level and obstructive sleep apnea or nocturnal intermittent hypoxia: K. Murase, et al.; PLoS One 8, e54184 (2013), 摘要;


9.

BK virus-associated nephropathy: neutrophil gelatinase-associated lipocalin as a new diagnostic tool?: S. Rau, et al.; Clin. Transplant. 27, E184 (2013), 摘要;


10.

Can neutrophil gelatinase-associated lipocalin help depict early contrast material-induced nephropathy?: A. Lacquaniti, et al.; Radiology 267, 86 (2013), 摘要;


11.

Circulating levels of neutrophil gelatinase-associated lipocalin (NGAL) correlate with the presence and severity of preeclampsia: S.M. Kim, et al.; Reprod. Sci. 20, 1083 (2013), 摘要;


12.

Day-to-day variation of urinary NGAL and rational for creatinine correction: J. Helmersson-Karlqvist, et al.; Clin. Biochem. 46, 70 (2013), 摘要;


13.

Differential expression of cancer-associated proteins in breastmilk: W. Qin, et al.; Breastfeed Med. 8, 120 (2013), 摘要;


14.

Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury: K. Kashani, et al.; Crit. Care 17, R25 (2013), 摘要;


15.

Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: an observational study: M. Di Nardo, et al.; Blood Purif. 35, 172 (2013), 摘要;


16.

Prognostic value of serum neutrophil gelatinase-associated lipocalin in metastatic and nonmetastatic colorectal cancer: J. Martí, et al.; World J. Surg. 37, 1103 (2013), 摘要;


17.

Prophylactic perioperative sodium bicarbonate to prevent acute kidney injury following open heart surgery: a multicenter double-blinded randomized controlled trial: M. Haase, et al.; PLoS Med. 10, e1001426 (2013), 摘要;


18.

Serum neutrophil gelatinase-associated lipocalin as a predictor of the development of bronchopulmonary dysplasia in preterm infants: H. Inoue, et al.; Early Hum. Dev. 89, 425 (2013), 摘要;


19.

The clinical utility of plasma neutrophil gelatinase-associated lipocalin in acute kidney injury: J.W. Pickering, et al.; Blood Purif. 35, 295 (2013), 摘要;


20.

The role of urinary liver-type fatty acid-binding protein in critically ill patients: E. Cho, et al.; J. Korean Med. Sci. 28, 100 (2013), 摘要;


21.

Urine Biomarkers Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Kidney Injury Molecule-1 (KIM-1) Have Different Patterns in Heart Failure Exacerbation: M. Park, et al.; Biomark. Insights 8, 15 (2013), 摘要;


22.

Albuminuria increases cystatin C excretion: implications for urinary biomarkers: M. Nejat, et al.; Nephrol. Dial. Transplant. 27 Suppl 3, iii96 (2012), 摘要;


23.

Are serum and urine neutrophil gelatinase-associated lipocalin predictive of renal graft function in short term?: N. Rahimzadeh, et al.; Pediatr. Transplant. 16, 796 (2012), 摘要;


24.

Association between systemic neutrophil gelatinase-associated lipocalin and anemia, relative hypochromia, and inflammation in chronic systolic heart failure: K. Shrestha, et al.; Congest. Heart Fail. 18, 239 (2012), 摘要;


25.

Delayed graft function (DGF) after living donor kidney transplantation: a study of possible explanatory factors: J. Salamzadeh, et al.; Ann. Transplant. 17, 69 (2012), 摘要;


26.

Evaluation of the impact of shock wave lithotripsy on kidneys using a new marker: how do neutrophil gelatinese-associated lypocalin values change after shock wave lithotripsy?: F. Zekey, et al.; Urology 80, 267 (2012), 摘要;


27.

Failure of remote ischemic preconditioning to reduce the risk of postoperative acute kidney injury in children undergoing operation for complex congenital heart disease: a randomized single-center study: K.R. Pedersen, et al.; J. Thorac. Cardiovasc. Surg. 143, 576 (2012), 摘要;


28.

Failure of remote ischemic preconditioning to reduce the risk of postoperative acute kidney injury in children undergoing operation for complex congenital heart disease: a randomized single-center study: K.R. Pedersen, et al.; J. Thorac. Cardiovasc. Surg. 143, 576 (2012), 摘要;


29.

Mild elevation of urinary biomarkers in prerenal acute kidney injury: K. Doi, et al.; Kidney Int. 82, 1114 (2012), 摘要;


30.

Neutrophil gelatinase-associated lipocalin as an early biomarker of acute kidney injury in liver transplantation: T.D. Jeong, et al.; Clin. Transplant. 26, 775 (2012), 摘要;


31.

Renal function and solitary kidney disease: Wilms tumour survivors versus patients with unilateral renal agenesis: J. Stefanowicz, et al.; Kidney Blood Press. Res. 35, 174 (2012), 摘要;


32.

Serum levels of gelatinase associated lipocalin as indicator of the inflammatory status in coronary artery disease: N. Kafkas, et al.; Int. J. Inflam. 2012, 189797 (2012), 摘要;


33.

Spironolactone diminishes urinary albumin excretion in patients with type 1 diabetes and microalbuminuria: a randomized placebo-controlled crossover study: S.E. Nielsen, et al.; Diabet. Med. 29, e184 (2012), 摘要;


34.

The effect of RAAS blockade on markers of renal tubular damage in diabetic nephropathy: u-NGAL, u-KIM1 and u-LFABP: S.E. Nielsen, et al.; Scand J. Clin. Lab Invest. 72, 137 (2012), 摘要;


35.

Urinary markers of kidney injury and kidney function decline in HIV-infected women: M.G. Shlipak, et al.; J. Acquir. Immune Defic. Syndr. 61, 565 (2012), 摘要;


36.

Urinary neutrophil gelatinase-associated lipocalin accurately detects acute allograft rejection among other causes of acute kidney injury in renal allograft recipients: N. Heyne, et al.; Transplantation 93, 1252 (2012), 摘要;


37.

Urinary NT-proBNP, NGAL, and H-FABP may predict hemodynamic relevance of patent ductus arteriosus in very low birth weight infants: V. Tosse, et al.; Neonatology 101, 260 (2012), 摘要;


38.

Clinical outcome of renal tubular damage in chronic heart failure: K. Damman, et al.; Eur. Heart J. 32, 2705 (2011), 摘要;


39.

Improved performance of urinary biomarkers of acute kidney injury in the critically ill by stratification for injury duration and baseline renal function: Z.H. Endre, et al.; Kidney Int. 79, 1119 (2011), 摘要;


40.

Kidney injury molecule-1 and N-acetyl-β-D-glucosaminidase in chronic heart failure: possible biomarkers of cardiorenal syndrome: C.G. Jungbauer, et al.; Eur. J. Heart. Fail. 13, 1104 (2011), 摘要;


41.

KIM-1 and NGAL: new markers of obstructive nephropathy: A. Wasilewska, et al.; Pediatr. Nephrol. 26, 579 (2011), 摘要;


42.

Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics: B.C. te Boekhorst, et al.; Cardiovasc. Res. 89, 680 (2011), 摘要;


43.

Neutrophil gelatinase-associated lipocalin at ICU admission predicts for acute kidney injury in adult patients: H.R. H. de Geus, et al.; Am. J. Respir. Crit. Care Med. 183, 907 (2011), 摘要;


44.

Neutrophil gelatinase-associated lipocalin concentrations predict development of acute kidney injury in neonates and children after cardiopulmonary bypass: C.D. Krawczeski, et al.; J. Pediatr. 158, 1009 (2011), 摘要;

45.

Renal dysfunction is a stronger determinant of systemic neutrophil gelatinase-associated lipocalin levels than myocardial dysfunction in systolic heart failure: K. Shrestha, et al.; J. Card. Fail. 17, 472 (2011), 摘要;


46.

Serum neutrophil gelatinase-associated lipocalin – a sensitive novel marker of renal impairment in liver cirrhosis?: A.L. Gerbes, et al.; Digestion 84, 82 (2011), 摘要;

47.

Temporal relationship and predictive value of urinary acute kidney injury biomarkers after pediatric cardiopulmonary bypass: C.D. Krawczeski, et al.; J. Am. Coll. Cardiol. 58, 2301 (2011), 摘要;


48.

Tubular markers do not predict the decline in glomerular filtration rate in type 1 diabetic patients with overt nephropathy: S.E. Nielsen, et al.; Kidney Int. 79, 1113 (2011), 摘要;


49.

Urinary neutrophil gelatinase-associated lipocalin and progression of diabetic nephropathy in type 1 diabetic patients in a four-year follow-up study: S.E. Nielsen, et al.; Nephron Clin. Pract. 118, c130 (2011), 摘要;


50.

Urinary neutrophil gelatinase-associated lipocalin as a marker of acute kidney injury after orthotopic liver transplantation: G. Wagener, et al.; Nephrol. Dial. Transplant. 26, 1717 (2011), 摘要;


51.

Urinary neutrophil gelatinase-associated lipocalin at birth predicts early renal function in very low birth weight infants: G. La Manna, et al.; Pediatr. Res. 70, 379 (2011), 摘要;


52.

Volume status and diuretic therapy in systolic heart failure and the detection of early abnormalities in renal and tubular function: K. Damman, et al.; J. Am. Coll. Cardiol. 57, 2233 (2011), 摘要;


53.

Bile duct ligation in developing rats: temporal progression of liver, kidney, and brain damage: J.M. Sheen, et al.; J. Pediatr. Surg. 45, 1650 (2010), 摘要;


54.

Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM1) in patients with diabetic nephropathy: a cross-sectional study and the effects of lisinopril: S.E. Nielsen, et al.; Diabet. Med. 27, 1144 (2010), 摘要;


55.

Neutrophil gelatinase-associated lipocalin (NGAL): validation of commercially available ELISA: K.R. Pedersen, et al.; Scand J. Clin. Lab Invest. 70, 374 (2010), 摘要;


56.

Pathological significance of a panel of urinary biomarkers in patients with drug-induced tubulointerstitial nephritis: Y. Wu, et al.; Clin. J. Am. Soc. Nephrol. 5, 1954 (2010), 摘要;


57.

Possible relationship between neutrophil gelatinase-associated lipocalin, hepcidin, and inflammation in haemodialysed patients: J. Malyszko, et al.; Nephron Clin. Pract. 115, c268 (2010), 摘要;


58.

Safety of low volume iodinated contrast administration for arteriovenous fistula intervention in chronic kidney disease stage 4 or 5 utilizing a bicarbonate prophylaxis strategy: E. Eisenhart, et al.; Semin. Dial. 23, 638 (2010), 摘要;


59.

Serum neutrophil gelatinase-associated lipocalin correlates with kidney function in heart allograft recipients: P. Przybylowski, et al.; Transplant Proc. 42, 1797 (2010), 摘要;


60.

Urinary neutrophil gelatinase-associated lipocalin as a novel biomarker for disease activity in lupus nephritis: T. Rubinstein, et al.; Rheumatology (Oxford) 49, 960 (2010), 摘要;


61.

Urinary neutrophil gelatinase-associated lipocalin: A potential biomarker for predicting rapid progression of drug-induced chronic tubulointerstitial nephritis: Y. Wu, et al.; Am. J. Med. Sci. 339, 537 (2010), 摘要;


62.

Urinary neutrophil-gelatinase associated lipocalin is a potential noninvasive marker for renal scarring in patients with vesicoureteral reflux: M. Ichino, et al.; J. Urol. 183, 2001 (2010), 摘要;


63.

Urine neutrophil gelatinase-associated lipocalin: an independent predictor of adverse outcomes in acute kidney injury: H.N. Yang, et al.; Am. J. Nephrol. 31, 501 (2010), 摘要;


64.


Implication of granulocyte-macrophage colony-stimulating factor induced neutrophil gelatinase-associated lipocalin in pathogenesis of rheumatoid arthritis revealed by proteome analysis: M. Katano, et al.; Arthritis Res. Ther. 11, R3 (2009), 摘要;


65.

NGAL: a new missing link between inflammation and uremic anemia?: D. Bolignano, et al.; Ren. Fail. 31, 622 (2009), 摘要;


66.

Comparison of the effects of gelatin and a modern hydroxyethyl starch solution on renal function and inflammatory response in elderly cardiac surgery patients: J. Boldt, et al.; Br. J. Anaesth. 100, 457 (2008), 摘要;


67.

Effect of a single intravenous immunoglobulin infusion on neutrophil gelatinase-associated lipocalin levels in proteinuric patients with normal renal function: D. Bolignano, et al.; J. Investig. Med. 56, 997 (2008), 摘要;


68.

Increased incidence of acute kidney injury with aprotinin use during cardiac surgery detected with urinary NGAL: G. Wagener, et al.; Am. J. Nephrol. 28, 576 (2008), 摘要;


69.

Neutrophil gelatinase-associated lipocalin as a biomarker of disease activity in pediatric lupus nephritis: M. Suzuki, et al.; Pediatr. Nephrol. 23, 403 (2008), 摘要;


70.

Neutrophil gelatinase-associated lipocalin expression in chronic myeloid leukemia: C. Villalva, et al.; Leuk. Lymphoma 49, 984 (2008), 摘要;


71.

Neutrophil gelatinase-associated lipocalin reflects the severity of renal impairment in subjects affected by chronic kidney disease: D. Bolignano, et al.; Kidney Blood Press. Res. 31, 255 (2008), 摘要;


72.


NGAL (neutrophil gelatinase-associated lipocalin) and cystatin C: are they good predictors of contrast nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine?: H. Bachorzewska-Gajewska, et al.; Int. J. Cardiol. 127, 290 (2008), 摘要;


73.

NGAL (neutrophil gelatinase-associated lipocalin) and cystatin C: are they good predictors of contrast nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine?: H. Bachorzewska-Gajewska, et al.; Int. J. Cardiol. 127, 290 (2008), 摘要;

74.

Serum neutrophil gelatinase-associated lipocalin as a marker of renal function in non-diabetic patients with stage 2-4 chronic kidney disease: J. Malyszko, et al.; Ren. Fail. 30, 625 (2008), 摘要;


75.

Urinary neutrophil gelatinase associated lipocalin (NGAL), a marker of tubular damage, is increased in patients with chronic heart failure: K. Damman, et al.; Eur. J. Heart Fail. 10, 997 (2008), 摘要;


76.

Urinary neutrophil gelatinase-associated lipocalin (NGAL) is associated with severity of renal disease in proteinuric patients: D. Bolignano, et al.; Nephrol. Dial. Transplant. 23, 414 (2008), 摘要;


77.

Urinary neutrophil gelatinase-associated lipocalin and acute kidney injury after cardiac surgery: G. Wagener, et al.; Am. J. Kidney Dis. 52, 425 (2008), 摘要;


78.

Urinary NGAL in premature infants: A.P. Lavery, et al.; Pediatr. Res. 64, 423 (2008), 摘要;


79.

Urine neutrophil gelatinase-associated lipocalin and interleukin-18 predict acute kidney injury after cardiac surgery: C. Xin, et al.; Ren. Fail. 30, 904 (2008), 摘要;


80.

Urine NGAL predicts severity of acute kidney injury after cardiac surgery: a prospective study: M. Bennett, et al.; Clin. J. Am. Soc. Nephrol. 3, 665 (2008), 摘要;


81.


Could neutrophil-gelatinase-associated lipocalin and cystatin C predict the development of contrast-induced nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine values?: H. Bachorzewska-Gajewska, et al.; Kidney Blood Press. Res. 30, 408 (2007), 摘要;


82.

Detection of subclinical tubular injury after renal transplantation: comparison of urine protein analysis with allograft histopathology: S. Schaub, et al.; Transplantation 84, 104 (2007), 摘要;


83.

Neutrophil gelatinase-associated lipocalin (NGAL) correlations with cystatin C, serum creatinine and eGFR in patients with normal serum creatinine undergoing coronary angiography: H. Bachorzewska-Gajewska, et al.; Nephrol. Dial. Transplant. 22, 295 (2007), 摘要;


84.

Neutrophil gelatinase-associated lipocalin in patients with autosomal-dominant polycystic kidney disease: D. Bolignano, et al.; Am. J. Nephrol. 27, 373 (2007), 摘要;


85.

Neutrophil-gelatinase-associated lipocalin and renal function after percutaneous coronary interventions: H. Bachorzewska-Gajewska, et al.; Am. J. Nephrol. 26, 287 (2006), 摘要;


其他参考文献

1.


Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), 摘要;


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