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Cell Navigator™TMR神经酰胺高尔基体染色试剂盒*红色荧光*
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货号 | 22752 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 100 Tests | 价格 | 3648 | |
| Ex (nm) | 544 | Em (nm) | 570 | |
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
高尔基体是大多数真核细胞胞质内囊泡和折叠膜的复合体，参与分泌和细胞内运输。它修饰内质网（ER）中内置的蛋白质和脂质，并准备将其运输到细胞外。它还在脂质在整个细胞中的运输和溶酶体的形成中起着重要作用。Cell Navigator™TMR神经酰胺高尔基染色试剂盒提供了一种简便，快速的方法，可以在活细胞中以红色荧光染色高尔基体。高尔基体通过形成相应的荧光代谢产物而被染色。Cell Navigator™TMR神经酰胺高尔基染色试剂盒提供了一种优化的测定方法，可用于通过荧光显微镜检查高尔基体的形态。
点击查看光谱
适用仪器
| 荧光显微镜 | |
| 激发： | Cy3/TRITC滤波片组 |
| 发射： | Cy3/TRITC滤波片组 |
| 推荐孔板： | 黑色透明 |
产品说明书
样品分析方案
概述
根据需要处理细胞
加入GGR169-神经酰胺工作溶液并在室温或37°C下孵育15至30分钟
添加染色缓冲液
使用Cy3滤波片组在显微镜下观察
储备溶液配制
向GGR-神经酰胺（组分A）中加入100 µL DMSO（组分C）制成GGR169-神经酰胺原液（100X）。
注意：将未使用的GGR-神经酰胺原液分装在-20°C下，以单次使用，避免冻融循环。
将10 µL GGR169-神经酰胺原液（100X）添加到990 µL染色缓冲液（组分B）中，制成GGR169-神经酰胺工作液。
可选：向1 mL GGR169-神经酰胺工作溶液中加入10 µL Hoechst 33342（组分D）以进行核染色。在带有DAPI滤光片组的荧光显微镜下观察。
操作步骤
- 接种并根据需要处理细胞。
- 将100 µL GGR169-神经酰胺工作溶液直接添加到细胞培养基中。
- 在室温或37°C下孵育15至30分钟。
- 除去GGR169-神经酰胺工作溶液，并用DPBS或您选择的缓冲液清洗一次。
- 加入100 µL /孔的染色缓冲液（组分B）。
- 在装有Cy3滤光片的荧光显微镜下观察。
图示
图1.HeLa细胞中GGR169神经酰胺高尔基染色的荧光图像。在37°C下，用100μL具有Hoechst 33342的GGR169-神经酰胺工作溶液对细胞染色20分钟。
参考文献
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Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids (2020): 158489
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Journal: FEBS letters (2019): 2366-2377
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Ceramide Transporter CERT Is Involved in Muscle Insulin Signaling Defects Under Lipotoxic Conditions.
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Journal: Diabetes (2018): 1258-1271
Ceramide-transfer protein-mediated ceramide transfer is a structurally tunable flow-inducing mechanism with structural feed-forward loops.
Authors: Giordano, Giulia
Journal: Royal Society open science (2018): 180494
Phosphoinositide binding by the PH domain in ceramide transfer protein (CERT) is inhibited by hyperphosphorylation of an adjacent serine-repeat motif.
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