Millipore Western化学发光HRP底物
Millipore Western化学发光HRP底物详细说明：
说明: Immobilon Western HRP 底物，2 x 50 mL
商标名: Immobilon
数量/包装: 2 x 50 mL
应用: Western blotting, dot/slot blotting
膜覆盖度，cm2: 1000
保存温度，°C: 2–8
试剂（体积）: 发光氨 （50 mL）； 过氧化物溶液 （50 mL）
保存条件: 2–8
体积，mL: 100
产品名称: Immobilon Western 化学发光 HRP 底物
过滤面积，cm2: 1000
详细产品资料也可和选购
KOD -Plus-系列酶（TOYOBO）KOD-201
| 特征1. 优良的保真性 | |
|
KOD -Plus-系列的酶具有强力3'→5'核酸外切酶活性（Proof-reading活性），相比KOD DNA polymerase，保真性更高，约为Taq DNA polymerase的80倍。zui适用于克隆。 |
特征2. 通过热启动提高了PCR反应效率
通过将2种抗KOD单克隆抗体预混在酶中，抑制了PCR反应前常温状态下的多聚酶活性和
3'→5'核酸外切酶活性（该活性是产生非特异性反应的主要原因）。抗体在PCR的zui初变
性步骤即发生失活，对扩增反应没有任何影响。
特征3. 延伸性?扩增效率的提高
对反应Buffer的组成进行了zui优化的重组，使之较以往的KOD DNA polymerase延伸性?扩
增效率均有很大的提高。
特征4. 超群的耐热性
因其耐热性比Taq DNA Polymerase更为优良，故可以将热变性步骤的温度设定值提高，对
GC-rich等容易形成高级结构的目的片段尤其适用。
特征5. 高PCR成功率【KOD -Plus- Ver.2】
对难扩增的目的片段、长目的片段的PCR成功率比KOD -plus-更高。另外，也降低了带入的
逆转录反应液对PCR反应的抑制。
KOD -Plus-系列酶（TOYOBO）KOD-201
＜几点建议＞
●PCR产物的克隆
由于本酶的PCR产物已被平滑化，可用平滑末端克隆法进行克隆。此时，如已对载体进行了脱磷酸化处理，就需要对PCR产物进行磷酸化，或使用带5'磷酸基的引物。 另外，由于本酶的PCR产物已被平滑化，不能直接进行TA克隆。可使用按以下方法开发的TA克隆专用试剂盒「TArget Clone -Plus-」。
●PCR条件的设定
酶的标准使用量为50μl反应体系1U。延伸反应在68℃条件下进行，按1kb目的片段1min.的标准。出现拖尾条带时，可降低MgSO4浓度，无法确认到扩增时，可提高MgSO4浓度。
●GC-rich模板的情况
在反应液中添加终浓度为2?5%的DMSO可得到改善。
●以RT反应液为模板的情况
过量带入RT反应液有可能会对PCR反应产生抑制作用。建议带入到PCR的RT反应液在PCR反应液的1/25以下（在1/50以下）。这样就无需考虑RT反应液中Mg2+、dNTPs的影响，只要按照基本反应条件添加本酶中添付的dNTPs和MgSO4即可。
|
品名 |
包装 |
保存温度 |
Code No. |
价格 |
|
KOD -Plus- |
200U×1 |
-20℃ |
KOD-201 |
RMB 800 |
|
（200U×1）×5 |
-20℃ |
KOD-201B |
RMB 3700 |
|
| KOD -Plus- Ver.2 |
200U×1 |
-20℃ |
KOD-211 |
RMB 1000 |
|
（200U×1）×5 |
-20℃ |
KOD-211B |
RMB 4600 |
相关产品价格表
| 品名 | 包装 | 保存温度 | Code No. | 价格 |
|
定点突变导入试剂盒 |
20次 |
-20℃ |
SMK-101 |
RMB 1500 |
|
KOD用TA克隆试剂盒 |
10次 |
-20℃ |
TAK-201 |
RMB 380 |
|
KOD -Plus-用Buffer |
1ml |
-20℃ |
KOD-2B |
RMB 50 |
|
KOD -Plus-用MgSO4 |
1ml |
-20℃ |
KOD-2S |
RMB 50 |
|
KOD -Plus- Ver.2用Buffer |
1ml |
-20℃ |
KOD-3B |
RMB 50 |
详细产品资料可和选购
GE Ficoll-Paque PLUS淋巴细胞分离液17-1440-02
用Ficoll-Paque PLUS从外周血分离人的淋巴细胞（交界处）。
* 仅限于科研使用。
• 无菌即用型试剂，适用于从外周血中分离淋巴细胞
• 能够保持B和T淋巴细胞的存活和分布状态
• 内毒素含量低（＜0.12 EU/ml）
每盒Ficoll-Paque PLUS均包含分步操作指南。
应用
Ficoll-Paque PREMIUM
用于从人外周血，骨髓和脐带血中分离单个核细胞
Ficoll-Paque PREMIUM 1.084
用于分离较高密度的人单核细胞，还可以用于分离小鼠和大鼠的血细胞
Ficoll-Paque PREMIUM 1.073
用于分离较低密度的人单核细胞, 如基质细胞或单核细胞
单核细胞分离手册
修订版的单核细胞分离手册（18-1152-69）介绍了用Ficoll-Paque介质对人单核细胞进行密度离心的技术方法。手册中详细介绍了应用Ficoll-Paque PLUS这种无菌即用型密度梯度介质的技术方法，该方法zui初用于从小量或是大量的人外周血中高得率纯化单个核细胞。同时手册中也介绍了用于制备临床用单个核细胞的Ficoll-Paque PREMIUM试剂的使用方法。
millipore聚碳酸酯滤膜47mm*0.4um HTTP04700
说明: Isopore 表面滤膜，聚碳酸酯，亲水，0.4 µm，47 mm，白色，光面
商标名: Isopore
数量/包装: 100
滤膜材质: Polycarbonate
滤膜商标名: Isopore
折射率: 1.6
23 °C 时的泡点: ≥2 bar
zui高操作温度，°C: 140
滤膜类型: 表面滤膜
可润湿性: 亲水
滤膜直径，mm: 47
millipore聚碳酸酯滤膜47mm*0.4um HTTP04700
Air Flow Rate, L/min x cm2/psi: 10
Water Flow Rate, mL/min x cm2/psi: 18
滤膜代码: HTTP
滤膜颜色: 白色
产品名称: Isopore 表面滤膜
滤膜表面: 光面
重量分析溶出物，%: <1
厚度，µm: 20
孔隙率 %: 5–20
详细产品信息可个选购
Description: The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximay 125 kDa transmembrane protein. The F4/80 antigen is expressed by a majority of mature macrophages and is the best marker for this population of cells. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development and is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo
| Host | Rat |
|---|---|
| Isotype | IgG2a, kappa |
| Reactivity | Mouse |
| Conjugate | PE |
| Laser | Blue Laser, Green Laser, Yellow-Green Laser |
| Emit | 575 nm |
| Excite | 488 – 561 nm |
| Reported Applications | Flow Cytometric Analysis |
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Amresco多粘菌素B硫酸盐POLYMYXIN B SULFATE
CAS Number:
1405-20-5
Synonym(s):
Aerosporin, Polysporin, Terramycin Ophthalmic
Molecular Weight:
N/A
Molecular Formula:
N/A
Storage Condition:
COLD
Hazard Statements:
H315 / H319 / H335
Precautionary Statements:
P280 / P302+P352 / P304+P340 / P305+P351+P338 / P312 / P403+P233
Hazard Code:
UN Number:
NONE
Recommended working concentration: 300 µg/ml
Bactericidal antibiotic that alters membrane permeability in gram-negative bacteria.
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RD Recombinant human IL-17A人白介17因子
DESCRIPTION
Source E. coliderived
Thr22Ala158
Accession # Q62386.1
Nterminal
Sequence
Analysis
Thr22
Structure / Form Disulfidelinked
homodimer
Predicted Molecular
Mass
15.5 kDa （monomer）
SPECIFICATIONS
Activity Measured by its ability to induce IL6
secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. （1995） Immunity 3:811.
The ED50 for this effect is typically 0.251.25
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >97%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
Interleukin 17 （also known as CTLA8）
is a T cellexpressed
pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of
herpesvirus Saimiri. cDNA clones encoding IL17
have been isolated from activated rat, mouse and human T cells. Mouse IL17
cDNA encodes a 158 amino acid （aa）
residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature IL17.
Both recombinant and natural IL17
have
been shown to exist as disulfide linked homodimers. At the amino acid level, mIL17
shows 57% and 87% sequence identity with herpesvirus and rat IL17,
respectively. An IL17
specific mouse cell surface receptor （IL17
R） has been cloned. While the expression of IL17
mRNA is restricted to activated alpha beta
TCR+CD4CD8T
cells, the expression of mIL17
R mRNA has been detected in virtually all cells and tissues tested. IL17
exhibits multiple biological activities on a
variety of cells including: the induction of IL6
and IL8
production in fibroblasts? the enhancement of surface expression of ICAM1
in fibroblasts? activation of NFκB
and costimulation of T cell proliferation.
References:
1. Kennedy, J. et al., （1996） J. Interferon Cytokine Res. 16:611.
2. Yao, Z. et al., （1995） J. Immunol. 155:5483.
3. Yao, Z. et al., （1995） Immunity 3:811.
4. Rouvier, E. et al., （1993） J. Immunol. 150:5445.
RD Recombinant human IL-17A人白介17因子 详细产品信息可和选购
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sigma Lidocaine利多卡因
| Product Name | Lidocaine, powder |
| Product Number | L7757 |
| Product Brand | SIGMA |
| CAS Number | 137-58-6 |
| Molecular Weight | 234.34 |
|
sigma Lidocaine利多卡因 TEST |
SPECIFICATION |
| Appearance （Color） | White |
| Appearance （Form） | Powder |
| Solubility （Color） | Colorless to Faint Yellow |
| Solubility （Turbidity） | Clear |
| 50 mg/ml, EtOH | |
| Carbon | 70.3 – 73.2 % |
| Nitrogen | 11.7 – 12.3 % |
| Infrared spectrum | Conforms to Structure |
| Purity （TLC） | ≥98 % |
| Recommended Retest Period | —————————— |
| 4 years | |
详细产品信息可本公司咨询
SIGMA Spantide II
| Product Name | Spantide II, ≥98% （HPLC） |
| Product Number | SCP0241 |
| Product Brand | SIGMA |
| Molecular Weight | 1597.86 |
| TEST | SPECIFICATION |
| Form | lyophilized powder |
| Appearance | White to off-white |
| Peptide Content | > or = to 65% |
| Purity by HPLC | > or = to 95% |
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P 物质乙酸盐水合物Substance P acetate salt hydrate
| Product Name | P 物质 乙酸盐 水合物, ≥95% （HPLC）, powder |
| Product Number | S6883 |
| Product Brand | SIGMA |
| CAS Number | 137348-11-9 （free base） |
| Molecular Weight | 1347.63 （anhydrous free base basis） |
| TEST | SPECIFICATION |
| Appearance （Color） | White |
| Appearance （Form） | Powder |
| Solubility （Color） |
Colorless
|
| Solubility （Turbidity） | Clear |
| at 1 mg/ml in 0.1 M HOAc | |
| Water （by Karl Fischer） | ≤10 % |
| % Acetate | ≤14.3 |
| % Peptide content | ≥75 |
| % Purity （HPLC） | ≥95 |
| Note | —————————— |
| 上海金畔生物科技有限公司代理sigma产品，咨询 上海金畔生物科技有限公司代理sigma产品，咨询 |
Formula and formula weight are for the free base.
|
Anti-pan Cytokeratin antibody [PCK-26] 鼠单克隆[PCK-26
Form
Liquid
Storage instructions
Store at +4°C short term （1-2 weeks）. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Anti-pan Cytokeratin antibody [PCK-26] 鼠单克隆[PCK-26
Purity
Ascites
Clonality
Monoclonal
Clone number
PCK-26
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） – pan Cytokeratin antibody [PCK-26] （ab6401）
（enlarge）
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） – pan Cytokeratin antibody [PCK-26] （ab6401）
（enlarge）
Western blot – pan Cytokeratin antibody [PCK-26] （ab6401）
（enlarge）
Show applications key
Our Abpromise guarantee covers the use of ab6401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Four stars（5 Abreviews）WB: Use at an assay depe…Read more →
WB: Use at an assay dependent dilution.
IHC-FoFr
IHC-FoFr: Use at an assa…Read more →
IHC-FoFr: Use at an assay dependent dilution.
IHC-P
Five stars（3 Abreviews）IHC-P: Use at an assay d…Read more →
IHC-P: Use at an assay dependent dilution.
IHC-Fr
Four stars（3 Abreviews）IHC-Fr: Use at an assay …Read more →
IHC-Fr: Use at an assay dependent dilution.
IF
IF: 1/300.
IF: 1/300.
Dot Blot
Dot: Use at an assay dep…Read more →
Dot: Use at an assay dependent dilution.
Flow Cyt
See more…Read more →
Flow Cyt: Use at an assay dependent dilution. PubMed: 19855980Use 10µl for 5 x 107 cells.
ICC/IF
Five stars（2 Abreviews）ICC/IF: 1/300.
ICC/IF: 1/300.
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin （mixture） is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization
Cytoplasmic
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） – pan Cytokeratin antibody [PCK-26] （ab6401）
ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P （Paraformaldehyde fixed paraffin embedded sections）. Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22°C. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody （1/500） in maleate buffer blocking solution for 16 hours at 22°C. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr Asha Seth
See Abreview
Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） – pan Cytokeratin antibody [PCK-26] （ab6401）
ab6401 staining pan Cytokeratin in mouse pancreatic ductal adenocarcinoma （left-hand panel） and mouse pancreatic neoplasia （right-hand panel） sections by immunohistochemistry （IHC-P – paraformaldehyde-fixed, paraffin-embedded sections）. Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; heat mediated antigen retrieval was performed. The sample was incubated with primary antibody （1/250） at 4°C for 8 hours. A Biotin-conjugated Goat polyclonal （1/1000） was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
See Abreview
Western blot – pan Cytokeratin antibody [PCK-26] （ab6401）
Observed band size : 55 kDa （why is the actual band size different from the predicted?）
All lanes: pan Cytokeratin antibody （ab6401） at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines （35 µg）
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal （1/5000） developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperature
This image is courtesy of an Abreview submitted by Pawel Mazur
See Abreview
Immunohistochemistry （Frozen sections） – pan Cytokeratin antibody [PCK-26] （ab6401）
ab6401 staining pan Cytokeratin in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry （IHC-Fr – frozen sections）. Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody （1/250 in PBS） for 8 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal （1/1000） was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
See Abreview
Immunocytochemistry/ Immunofluorescence – pan Cytokeratin antibody [PCK-26] （ab6401）
ab6401 staining pan Cytokeratin in Human Panc1 cells by ICC/IF （Immunocytochemistry/immunofluorescence）. Cells were fixed with acetone/methanol （1:1） and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody （1/300 in PBS） for 2 hours. An Alexa Fluor®488-conjugated DGoat anti-mouse IgG polyclonal （1/1000） was used as the secondary antibody. Nuclei were counterstained with DAPI.
This image is courtesy of an anonymous Abreview
See Abreview
Anti-pan Cytokeratin antibody [PCK-26] 鼠单克隆[PCK-26 详细产品信息可和选购
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Anti-CD11b antibody [EP1345Y] CD11兔单克隆抗体
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1345Y
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Murine Lineage Negative
Stem Cells >> Mesenchymal Stem Cells >> Negative Markers
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Western blot – CD11b antibody [EP1345Y] （ab52478）
（enlarge）
Immunohistochemistry （Paraffin-embedded sections） – CD11b antibody [EP1345Y] （ab52478）
（enlarge）
Flow Cytometry-CD11b antibody [EP1345Y]（ab52478）
（enlarge）
Show applications key
Our Abpromise guarantee covers the use of ab52478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
WB: 1/20000 – 1/50000. P…Read more →
WB: 1/20000 – 1/50000. Predicted molecular weight: 128 kDa.
IP
IP: 1/80.
IP: 1/80.
ICC
ICC: 1/100 – 1/250.
ICC: 1/100 – 1/250.
Flow Cyt
Flow Cyt: 1/20 – 1/50.
Flow Cyt: 1/20 – 1/50.
IHC-P
Anti-CD11b antibody [EP1345Y] CD11兔单克隆抗体 详细产品信息可和选购
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sigma 顺铂cis-Diamineplatinum（II） dichloride
__T_E_S_T______________________________________________S_p_e_c_if_ic_a_t_io_n______________________
Appearance （Color） Yellow to Orange
Appearance （Form） Conforms to Requirements
Powder or Crystals
Infrared spectrum Conforms to Structure
Nitrogen 9.2- 9.5 %
ICP Major Analysis Confirmed
Confirms Platinum Component
Purity Meets Requirements
>=99.9% Based on Trace Metals Analysis
Trace Metal Analysis 1,000.0 ppm
Remarks:
Specification
sigma 顺铂cis-Diamineplatinum（II） dichloride
详细产品信息可和订购
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For 50 preps: AllPrep DNA Spin Columns, RNeasy Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers
AllPrep DNA/RNA/Protein Mini Kit /DNA RNA 蛋白质纯化试剂盒详细产品信息可和选购
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sigma红细胞裂解液Red Blood Cell Lysing Buffer Hybri-Max™
| Product Name | Red Blood Cell Lysing Buffer Hybri-Max™, liquid, sterile-filtered, suitable for hybridoma |
| Product Number | R7757 |
| Product Brand | SIGMA |
|
sigma红细胞裂解液Red Blood Cell Lysing Buffer Hybri-Max™ |
|
| TEST | SPECIFICATION |
| Appearance （Turbidity） | Clear |
| Appearance （Form） | Liquid |
| pH | 7.3 – 7.7 |
| Osmolality | 281 – 311 mOs/kg |
| Sterility | Pass |
| Endotoxin Level | <= 1 EU/ml |
| Splenocyte Separation | Pass |
sigma红细胞裂解液Red Blood Cell Lysing Buffer Hybri-Max™详细产品信息可和选购
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Recombinant Mouse M-CSF巨噬细胞集落刺激因子
MCSF,
also known as CSF1,
is a fourαhelicalbundle
cytokine that is the primary regulator of macrophage survival, proliferation and differentiation （1 3）.
MCSF
is also essential for the survival and proliferation of osteoclast progenitors （1, 4）. MCSF
also primes and enhances macrophage killing of tumor cells and
microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis （2, 3）. MCSF
increases during
pregnancy to support implantation and growth of the decidua and placenta （5）. Sources of MCSF
include fibroblasts, activated macrophages, endometrial secretory
epithelium, bone marrow stromal cells and activated endothelial cells （1 5）.
The MCSF
receptor （cfms）
transduces its pleotropic effects and mediates its
endocytosis. MCSF
mRNAs of various sizes occur （3 9）.
Full length mouse MCSF
transcripts encode a 520 amino acid （aa） type I transmembrane （TM） protein with
a 462 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two
proteolytically cleaved, secreted dimers. One is an Nand
Oglycosylated
86 kDa dimer, while the other is modified by both glycosylation and chondroitinsulfate
proteoglycan （PG） to generate a 200 kDa subunit. Although PGmodified
MCSF
can circulate, it may be immobilized by attachment to type V collagen （8）. Shorter
transcripts encode M CSF
that lacks cleavage and PG sites and produces an Nglycosylated
68 kDa TM dimer and a slowly produced 44 kDa secreted dimer （7）.
Although forms may vary in activity and halflife,
all contain the Nterminal
150 aa portion that is necessary and sufficient for interaction with the M CSF
receptor
（10, 11）. The first 229 aa of mature mouse MCSF
shares 87%, 83%, 82% and 81% aa identity with corresponding regions of rat, dog, cow and human MCSF,
respectively （12, 13）. Human MCSF
is active in the mouse, but mouse MCSF
is reported to be speciesspecific
Recombinant Mouse M-CSF巨噬细胞集落刺激因子
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