Amplite 比色法BCA蛋白定量分析试剂盒

简要概述

传统的BCA蛋白质检测法广泛用于定量蛋白质。但是，它的检测效率低下，例如，您必须在37°C下加热BCA反应30分钟，或者在室温下等待两个小时。 Amplite 比色法BCA蛋白定量测定试剂盒是一种用于检测总蛋白浓度的拥有两组分且与洗涤剂兼容的检测试剂盒。该检测基于相同的铜螯合反应，可提供与传统BCA蛋白质检测相当的准确性，传统BCA蛋白质检测可在高温或更长的孵育时间下进行。在560 nm附近检测蛋白质信号，并在30分钟内完成测定。它方便，快速且稳定，无需高温。 Amplite 比色BCA蛋白定量分析试剂盒可以以方便的96孔板进行操作，无需分离步骤即可轻松实现自动化。

产品说明书

样品分析方案

概述

标准溶液制备：

BSA标准溶液：

将160 µL的1 mg / mL BSA标准溶液（组分C）添加到240 µL的PBS（未提供）中，以生成400 µg / mL的BSA标准溶液（BS1）。然后在PBS中使用1：2的系列稀释液以获得系列稀释的BSA标准品（BS2-BS7）。注意：在每次测定中必须创建标准曲线。

工作溶液制备

BCA工作溶液：

1.通过将50份BCA溶液A（组分A）与1份BCA溶液B（组分B）（50：1，溶液A：B的体积比）混合，制备所需的BCA工作溶液量。
2.充分混合，直到BCA工作溶液为均匀的浅绿色。
注意：1 mL BCA工作溶液足以进行20次检测。

操作步骤

表1.在透明的底96孔板中BSA标准品和测试样品的布局BS = BSA标准品（BS1-BS7，400至6.25 µg / mL）; BL =空白控件； TS =测试样品

图示

图1.使用透明的底96孔板，使用Amplite比色BCA蛋白定量分析试剂盒测量BSA剂量响应。

参考文献

Specialized Cell-Free DNA Blood Collection Tubes Can Be Repurposed for Extracellular Vesicle Isolation: A Pilot Study.
Authors: Heatlie, Jessica and Chang, Vanessa and Fitzgerald, Sandra and Nursalim, Yohanes and Parker, Kate and Lawrence, Ben and Print, Cristin G and Blenkiron, Cherie
Journal: Biopreservation and biobanking (2020)

Protein Quantitation and Analysis of Purity.
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Journal: Methods in molecular biology (Clifton, N.J.) (2017): 225-255

Proteomic Approach for Extracting Cytoplasmic Proteins from Streptococcus sanguinis using Mass Spectrometry.
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[iTRAQ technology combined with 2D-LC-MS/MS to analyze effect of Coptidis Rhizoma on cytochrome P450 isoenzyme expression].
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Total protein quantitation using the bicinchoninic acid assay and gradient elution moving boundary electrophoresis.
Authors: Kralj, Jason G and Munson, Matthew S and Ross, David
Journal: Electrophoresis (2014): 1887-92

Quantitative evaluation of proteins with bicinchoninic acid (BCA): resonance Raman and surface-enhanced resonance Raman scattering-based methods.
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Journal: The Analyst (2012): 5834-8

Quantitation of protein.
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Journal: Methods in enzymology (2009): 73-95

Interferences of suspended clay fraction in protein quantitation by several determination methods.
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Journal: Analytical biochemistry (2008): 108-14

A comparison of protein quantitation assays for biopharmaceutical applications.
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Journal: Molecular biotechnology (2007): 99-111

Biochemical characterization of recombinant mouse amelogenins: protein quantitation, proton absorption, and relative affinity for enamel crystals.
Authors: Ryu, O H and Hu, C C and Simmer, J P
Journal: Connective tissue research (1998): 207-14; discussion 241-6

Cy5- dUTP *在Tris缓冲液（pH 7.5）中为1 mM *

	货号	17026	存储条件	在零下15度以下保存, 避免光照
	规格	25 nmoles	价格	1176
	Ex (nm)	651	Em (nm)	670
	分子量	1176.04	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17026

产品名称：Cy5- dUTP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：1176.04

溶剂：水

激发波长(nm)：651

发射波长(nm)：670

消光系数(nm):250000

校正因子(280 nm)：0.03

量子产率：0.27（PBS中），0.4（乙醇中）

产品介绍

染料修饰的脱氧尿苷5′-三磷酸酯（例如氨基烯丙基-dUTP）可用于通过常规酶促掺入方法（例如逆转录，缺口翻译，随机引物标记或PCR）生产含染料的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。此Cy5-dUTP缀合物可与SpectrumFRed 滤波片组一起使用，产生红色荧光（SpectrumFRed 是Vysis的商标）。它在功能上等效于Cyanine 5-dUTP（珀金埃尔默（Perkin Elmer）的NEL579001EA）。

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参考文献

Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.
Authors: Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo
Journal: BMC cancer (2018): 772

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
Authors: Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V
Journal: Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.
Authors: Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N
Journal: Scientific reports (2017): 6674

Separation of replication and transcription domains in nucleoli.
Authors: Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I
Journal: Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.
Authors: Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V
Journal: Gene (2013): 138-42

[Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions].
Authors: Li, You-Zhi and Chen, Zhi-Yao and Wang, Hui and Huang, Huan and Song, Qin-Xin and Zhou, Guo-Hua
Journal: Zhonghua nan ke xue = National journal of andrology (2012): 109-14

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

Time-resolved three-dimensional molecular tracking in live cells.
Authors: Wells, Nathan P and Lessard, Guillaume A and Goodwin, Peter M and Phipps, Mary E and Cutler, Patrick J and Lidke, Diane S and Wilson, Bridget S and Werner, James H
Journal: Nano letters (2010): 4732-7

[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5

生物素-11-dGTP

	货号	17015	存储条件	在零下15度以下保存, 避免光照
	规格	25 nmoles	价格	1176
	Ex (nm)		Em (nm)
	分子量	898.71	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17015

产品名称：生物素-11-dGTP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：898.71

溶剂：水

产品介绍

生物素修饰的脱氧鸟苷5′-三磷酸酯广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3′-末端标记。可以通过切口平移，随机引物，3′-末端末端标记或PCR的方法将生物素11-dGTP酶促地掺入DNA中。数字“ 11”是dGTP与生物素之间连接子骨架中的碳原子数。连接子越长，生物素与抗生物素蛋白的相互作用越有效。另一方面，接头越短，dGTP就越有效地掺入DNA。建议链接器’11’的长度对于大多数应用而言是最佳的。 Biotin-11-dGTP用于在各种杂交应用中产生生物素化的DNA探针，包括Southern印迹，Northern印迹，斑点印迹，固定细胞和组织。它在化学上等同于PerkinElmer（PE）的NEL541001EA。

参考文献

Base-Excision-Repair-Induced Construction of a Single Quantum-Dot-Based Sensor for Sensitive Detection of DNA Glycosylase Activity.
Authors: Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang
Journal: Analytical chemistry (2016): 7523-9

PRINS for mapping single-copy genes.
Authors: Tharapel, Avirachan T and Wachtel, Stephen S
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 59-67

Chromosomal localization of single copy genes SRY and SOX3 by primed in situ labeling (PRINS).
Authors: Kadandale, J S and Tunca, Y and Tharapel, A T
Journal: Microbial & comparative genomics (2000): 71-4

Quantification of telomerase activity by direct scintillation counting.
Authors: Kazmer, S and Pan, K M and Vassilev, L
Journal: Journal of biochemical and biophysical methods (1999): 113-7

Telomerase assay using biotinylated-primer extension and magnetic separation of the products.
Authors: Sun, D and Hurley, L H and Von Hoff, D D
Journal: BioTechniques (1998): 1046-51

Universal cloning and direct sequencing of rearranged antibody V genes using C region primers, biotin-captured cDNA and one-side PCR.
Authors: Heinrichs, A and Milstein, C and Gherardi, E
Journal: Journal of immunological methods (1995): 241-51

Purification of DNA fragments containing excision-repair patches from human cells using streptavidin-biotin.
Authors: Larone, G E and Hunting, D J
Journal: Mutation research (1991): 273-80

ReadiUse 预激活的APC-iFluor 800串联染料

	货号	2572	存储条件	Multiple
	规格	1 mg	价格	4272
	Ex (nm)	804	Em (nm)	660
	分子量		溶剂
	产品详细介绍

简要概述

藻蓝蛋白（APC）是一种蓝藻藻螺旋藻中分离的藻胆蛋白。像其他藻胆蛋白一样，APC是荧光的，具有极高的吸收率和高量子效率。我们的APC-iFluor 800串联探针拥有一种独特的荧光团，具有用于光谱流式细胞术的IR发射。它的发射光谱不与任何常见的荧光团重叠，从而为流式细胞术中的多色应用提供了独特的颜色。 ReadiUse 预活化的APC-iFluor 800串联蛋白是一种活化的APC蛋白，可以轻松偶联到抗体上，具有比常规APC串联更高的结合产率。

产品说明书

钙离子荧光探针Cal-520L 马来酰亚胺

	货号	20611	存储条件	在零下15度以下保存, 避免光照
	规格	100 ug	价格	5136
	Ex (nm)	493	Em (nm)	515
	分子量	991.78	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：20611

产品名称：钙离子荧光探针Cal-520L 马来酰亚胺

规格：100 ug

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：991.78

溶剂：DMSO

激发波长(nm)：493

发射波长(nm)：515

荧光量子产率：0.75

产品介绍

钙测量对于许多生物学研究至关重要，一般结合钙后光谱响应的荧光探针使研究人员能够通过使用荧光显微镜，流式细胞仪，荧光光谱和荧光酶标仪来研究细胞内游离钙浓度的变化。作为低亲和力钙指示剂，Cal-520L已被证明是最好的绿色荧光钙指示剂之一。该硫醇反应性的Cal-520L马来酰亚胺可与IgG或其他载体分子偶联，以制备钙敏感生物共轭物，以检测特定靶标的钙空间变化。

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参考文献

Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision.
Authors: Makowka, Philipp and Bruegmann, Tobias and Dusend, Vanessa and Malan, Daniela and Beiert, Thomas and Hesse, Michael and Fleischmann, Bernd K and Sasse, Philipp
Journal: Nature communications (2019): 1281

Polymer microchamber arrays for geometry-controlled drug release: a functional study in human cells of neuronal phenotype.
Authors: Kopach, Olga and Zheng, Kayiu and Sindeeva, Olga A and Gai, Meiyu and Sukhorukov, Gleb B and Rusakov, Dmitri A
Journal: Biomaterials science (2019): 2358-2371

A Water Framework Directive-compatible metric for assessing acidification in UK and Irish rivers using diatoms.
Authors: Juggins, Steve and Kelly, Martyn and Allott, Tim and Kelly-Quinn, Mary and Monteith, Don
Journal: The Science of the total environment (2016): 671-678

Mapping Calcium-Sensitive Regions in the Neuronal Calcium Sensor GCAP2 by Site-Specific Fluorescence Labeling.
Authors: Sulmann, Stefan and Wallisch, Melanie and Scholten, Alexander and Christoffers, Jens and Koch, Karl-Wilhelm
Journal: Biochemistry (2016): 2567-77

Red-Shifted Aequorin Variants Incorporating Non-Canonical Amino Acids: Applications in In Vivo Imaging.
Authors: Grinstead, Kristen M and Rowe, Laura and Ensor, Charles M and Joel, Smita and Daftarian, Pirouz and Dikici, Emre and Zingg, Jean-Marc and Daunert, Sylvia
Journal: PloS one (2016): e0158579

Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein.
Authors: Yang, Sung-Tae and Lim, Sung In and Kiessling, Volker and Kwon, Inchan and Tamm, Lukas K
Journal: Scientific reports (2016): 32866

Is FEV(1) an indicator of low bone mineral density in adults? The Fifth Korea National Health and Nutrition Examination Survey.
Authors: Lee, Kayoung
Journal: Journal of bone and mineral metabolism (2015): 335-41

Excited-state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging.
Authors: Oscar, Breland G and Liu, Weimin and Zhao, Yongxin and Tang, Longteng and Wang, Yanli and Campbell, Robert E and Fang, Chong
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 10191-6

Optogenetic control of ROS production.
Authors: Wojtovich, Andrew P and Foster, Thomas H
Journal: Redox biology (2014): 368-76

Copper-64 radiolabelling of the C2A domain of synaptotagmin I using a functionalised bis(thiosemicarbazone): A pre- and post-labelling comparison.
Authors: Hueting, Rebekka and Tavaré, Richard and Dilworth, Jonathan R and Mullen, Gregory E
Journal: Journal of inorganic biochemistry (2013): 108-11

荧光素醛[5-FAM醛]

	货号	9000	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	3648
	Ex (nm)	493	Em (nm)	517
	分子量	550.52	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：9000

产品名称：荧光素醛[5-FAM醛]

规格：5 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：445.43

溶剂：DMSO

激发波长(nm)：493

发射波长(nm)：517

消光系数（cm-1 M-1）:83000

校正因子（260nm）:0.32

校正因子 (280 nm)：0.178

产品介绍

荧光素醛是可以与胺，肼或羟胺反应的反应性荧光染料。荧光素是使用最广泛的绿色荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物缀合反应有时需要的条件。醛与胺基反应形成席夫碱（Schiff base）。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。共轭荧光素染料可以很容易地通过普通的荧光仪器在FITC通道下检测到。

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参考文献

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982

Bisretinoid phospholipid and vitamin A aldehyde: Shining a light.
Authors: Kim, Hye Jin and Sparrow, Janet R
Journal: Journal of lipid research (2020)

Characterization of ascites-derived aldehyde dehydrogenase-positive ovarian cancer stem cells isolated from Leghorn chickens.
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214

Citral Inhibition of Human Salivary Aldehyde Dehydrogenase.
Authors: Laskar, Amaj Ahmed and Khan, Masood Alam and Ahmad, Sumbul and Hashmi, Amiruddin and Younus, Hina
Journal: Cell biochemistry and biophysics (2020): 31-42

Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl-selenium chemistry.
Authors: Lee, Woohyun and Mulay, Sandip V and Shimodaira, Shingo and Abdillah, Ariq and Palma, Jaymee and Kim, Youngsam and Yudhistira, Tesla and Churchill, David G
Journal: Methods in enzymology (2020): 267-289

Establishing sample-preparation protocols for X-ray phase-contrast CT of rodent spinal cords: Aldehyde fixations and osmium impregnation.
Authors: Barbone, Giacomo E and Bravin, Alberto and Mittone, Alberto and Kraiger, Markus J and Hrabě de Angelis, Martin and Bossi, Mario and Ballarini, Elisa and Rodriguez-Menendez, Virginia and Ceresa, Cecilia and Cavaletti, Guido and Coan, Paola
Journal: Journal of neuroscience methods (2020): 108744

Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling.
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300

Isocyanoacetate-Aldehyde Polymerization: A Facile Tool toward Functional Oxazoline-Containing Polymers.
Authors: Cheng, Tianyu and Chen, Yizhao and Ding, Jie and Qin, Anjun and Tang, Ben Zhong
Journal: Macromolecular rapid communications (2020): e2000179

Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912

罗丹明醛[5-TAMRA醛]

	货号	9005	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	3648
	Ex (nm)	552	Em (nm)	578
	分子量	536.03	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：9005

产品名称：罗丹明醛[5-TAMRA醛]

规格：5 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：536.03

溶剂：DMSO

激发波长(nm)：552

发射波长(nm)：578

消光系数（cm-1 M-1）:90000

校正因子（260nm）：0.32

校正因子 (280 nm)：0.178

产品介绍

罗丹明醛是一种可以与胺，肼或羟胺反应的活性荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物缀合反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。可以通过普通的荧光仪器在TRITC或Cy3通道下轻松检测缀合罗丹明染料。

点击查看光谱

参考文献

A novel rhodamine-based colorimetric and fluorometric probe for simultaneous detection of multi-metal ions.
Authors: Zhang, Yuqi and Wang, Wang and Li, Ran and Zhang, Ensheng and Li, Zhonghua and Tang, Long and Han, Bo and Hou, Xiufang and Wang, Ji-Jiang
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 118050

Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982

Super-Resolution Fluorescence Imaging of Arabidopsis thaliana Transfer Cell Wall Ingrowths using Pseudo-Schiff Labelling Adapted for the Use of Different Dyes.
Authors: Rae, Angus E and Wei, Xiaoyang and Flores-Rodriguez, Neftali and McCurdy, David W and Collings, David A
Journal: Plant & cell physiology (2020): 1775-1787

A rhodamine-based turn-on nitric oxide sensor in aqueous medium with endogenous cell imaging: an unusual formation of nitrosohydroxylamine.
Authors: Alam, Rabiul and Islam, Abu Saleh Musha and Sasmal, Mihir and Katarkar, Atul and Ali, Mahammad
Journal: Organic & biomolecular chemistry (2018): 3910-3920

Fluorescent probes for detecting cysteine.
Authors: Yan, Fanyong and Sun, Xiaodong and Zu, Fanlin and Bai, Zhangjun and Jiang, Yingxia and Fan, Keqing and Wang, Jie
Journal: Methods and applications in fluorescence (2018): 042001

Inhibitory Effects of Benzaldehyde, Vanillin, Muscone and Borneol on P-Glycoprotein in Caco-2 Cells and Everted Gut Sac.
Authors: Wang, Shixiang and Tan, Nan and Ma, Cuicui and Wang, Jie and Jia, Pu and Liu, Jianghong and Yang, Yang and Xie, Zhanxiong and Zhao, Kun and Zheng, Xiaohui
Journal: Pharmacology (2018): 269-277

Reinvestigation of an O-Salicylaldehyde Ester Functional Group in Aqueous Buffer and Discovery of a Coumarin Scaffold Probe for Selective N-Terminal Cysteine Labeling.
Authors: Murale, Dhiraj P and Hong, Seong Cheol and Jang, Se-Young and Lee, Jun-Seok
Journal: Chembiochem : a European journal of chemical biology (2018): 2545-2549

Hyaluronate-Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer.
Authors: Beack, Songeun and Cho, Minsoo and Kim, Young-Eun and Ahn, G-One and Hahn, Sei Kwang
Journal: Bioconjugate chemistry (2017): 1434-1442

The Protective Effect of Jatrorrhizine Against Oxidative Stress in Primary Rat Cortical Neurons.
Authors: Luo, Tao and Shen, Xiu-Yin and Li, Sheng and Ouyang, Ting and Mai, Quan-An and Wang, Hua-Qiao
Journal: CNS & neurological disorders drug targets (2017): 617-623

Tunable uptake/release mechanism of protein microgel particles in biomimicking environment.
Authors: Pepe, A and Podesva, P and Simone, G
Journal: Scientific reports (2017): 6014

Cy5醛

	货号	9011	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	3648
	Ex (nm)	651	Em (nm)	670
	分子量	945.04	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：9011

产品名称：Cy5醛

规格：5 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：839.94

溶剂：DMSO

激发波长(nm)：651

发射波长(nm)：670

消光系数（cm-1 M-1）:250000

荧光量子产率：0.27（PBS）,0.4(乙醇)

校正因子 (280 nm)：0.03

产品介绍

Cy5醛是一种可与胺，肼或羟胺反应的活性红色荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物缀合反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。缀合Cy5染料可以通过Cy5通道下的普通荧光仪器轻松检测到。

点击查看光谱

参考文献

[Microanalysis of Acetaldehyde Dehydrogenase 2 Genotype Based on SNaPShot-FCA].
Authors: Zeng, Shu-Shu and Gao, Xiu-Feng and Zhang, You and Feng, Chuan-Rui and Luo, Ya-Xiong and Wu, Xiao-Ting and Li, Yong-Sheng
Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (2019): 737-742

pH responsible and fluorescent Cy5.5-PEG-g-A-HA/CDDP complex nanoparticles: synthesis, characterization, and application for targeted drug delivery.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Liu, Chun and Zhang, Qiqing
Journal: Journal of materials science. Materials in medicine (2019): 58

Tumoral Acidic pH-Responsive cis-Diaminodichloroplatinum-Incorporated Cy5.5-PEG- g-A-HA Nanoparticles for Targeting Delivery of CDDP against Cervical Cancer.
Authors: Cheng, Cui and Meng, Yabin and Zhang, Zhihong and Li, Ya and Zhang, Qiqing
Journal: ACS applied materials & interfaces (2018): 26882-26892

Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance.
Authors: Zhang, Hua and Zhao, Zhen and Lei, Zhen and Wang, Zhenxin
Journal: Analytical chemistry (2016): 11358-11363

Sensitive detection of histamine using fluorescently labeled oxido-reductases.
Authors: Gustiananda, Marsia and Andreoni, Alessio and Tabares, Leandro C and Tepper, Armand W J W and Fortunato, Luca and Aartsma, Thijs J and Canters, Gerard W
Journal: Biosensors & bioelectronics (2012): 419-25

An efficient strategy for sequencing-by-synthesis.
Authors: Gao, Li and Lu, Hua and Xu, Lijian and Lu, Zuhong
Journal: Journal of nanoscience and nanotechnology (2010): 2988-93

Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.
Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
Journal: The Biochemical journal (2010): 49-59

Protein thiol oxidation in murine airway epithelial cells in response to naphthalene or diethyl maleate.
Authors: Spiess, Page C and Morin, Dexter and Williams, Chase R and Buckpitt, Alan R
Journal: American journal of respiratory cell and molecular biology (2010): 316-25

Chemical derivatization of compact disc polycarbonate surfaces for SNPs detection.
Authors: Bañuls, María-José and García-Piñón, Francisco and Puchades, Rosa and Maquieira, Angel
Journal: Bioconjugate chemistry (2008): 665-72

Identification and characterization of Clostridium perfringens using single target DNA microarray chip.
Authors: Al-Khaldi, Sufian F and Villanueva, Doralis and Chizhikov, Vladimir
Journal: International journal of food microbiology (2004): 289-96

Buccutite AP（碱性磷酸酶）抗体偶联试剂盒*针对标记25 ug蛋白进行了优化*

简要概述

蛋白质-蛋白质缀合交联剂通常使用在每个末端具有不同反应性的双功能接头（例如SMCC）连接两个不同的蛋白质。交联剂的一端与赖氨酸和N端的胺（-NH2）反应（通过NHS酯），另一端与半胱氨酸的硫醇基（-SH）反应（通过马来酰亚胺）。但是，SMCC修饰的蛋白质极不稳定，通常会自反应，因为蛋白质包含多个胺基和硫醇基，会引起大量的交联。另外，定量蛋白质中的马来酰亚胺基团的数量是非常困难的。 Buccutite AP（碱性磷酸酶）抗体偶联试剂盒旨在快速制备具有预活化AP的AP偶联物。该试剂盒针对标记25 ug蛋白进行了优化。 Buccutite FOL活化的AP可以在极其温和的中性条件下与Buccutite MTA修饰的抗体轻松反应，而无需任何催化剂。与常用的SMCC和其他类似技术相比，Buccutite 生物缀合系统更加稳定并且易于使用。它能够以更高的效率和产量更快且定量地偶联生物分子。

图示

图1.使用Buccutite AP（碱性磷酸酶）抗体偶联试剂盒制备的ALP-山羊-抗小鼠IgG偶联物检测了小鼠IgG剂量曲线。将3倍系列稀释的小鼠IgG包被在96孔板上，然后按照标准ELISA方法，向每个孔中加入100uL GAM IgG-ALP偶联物（1ug / ml）。在孵育30分钟后，将pNPP底物溶液用于检测固定的小鼠IgG，并在400 nm处读数。

参考文献

Direct pulp capping with mineral trioxide aggregate: an immunohistologic comparison with calcium hydroxide in rodents.
Authors: Dammaschke, Till and Stratmann, Udo and Wolff, Philipp and Sagheri, Darius and Schäfer, Edgar
Journal: Journal of endodontics (2010): 814-9

Vitamins C and E inhibit apoptosis of cultured human term placenta trophoblast.
Authors: Tannetta, D S and Sargent, I L and Linton, E A and Redman, C W G
Journal: Placenta (2008): 680-90

Anti-alkaline phosphatase antibody positive myasthenia gravis.
Authors: Konishi, Tetsuro and Ohta, Kiyoe and Shigemoto, Kazuhiro and Ohta, Mitsuhiro
Journal: Journal of the neurological sciences (2007): 89-93

Ectopic expression of alkaline phosphatase in proximal tubular brush border membrane of human renal cell carcinoma.
Authors: Prasad, R and Lambe, S and Kaler, P and Pathania, S and Kumar, S and Attri, S and Singh, S K
Journal: Biochimica et biophysica acta (2005): 240-5

[Identification of antigens of Colombian Giardia duodenalis isolates recognized by total IgG and subclasses].
Authors: Hernández, Jenny Fabiola and Duque, Sofía and Arévalo, Adriana and Guerrero, Rafael and Nicholls, Rubén Santiago
Journal: Biomedica : revista del Instituto Nacional de Salud (2003): 263-73

Primary intrapelvic seminoma in Klinefelter’s syndrome.
Authors: Kurabayashi, A and Furihata, M and Matsumoto, M and Sonobe, H and Ohtsuki, Y and Aki, M and Kuwahara, M
Journal: Pathology international (2001): 624-8

Recombinant adenoviral vector-lipofectAMINE complex for gene transduction into human T lymphocytes.
Authors: Di Nicola, M and Milanesi, M and Magni, M and Bregni, M and Carlo-Stella, C and Longoni, P and Tomanin, R and Ravagnani, F and Scarpa, M and Jordan, C and Gianni, A M
Journal: Human gene therapy (1999): 1875-84

A nonradioactive method for localization of endothelin receptor mRNA in situ.
Authors: McEwan, P E and Valdenaire, O and Sutherland, L and Webb, D J and Gray, G A
Journal: Journal of cardiovascular pharmacology (1998): S443-6

Focus luminescence assay: macroscopically visualized foci of human cytomegalovirus and varicella zoster virus infection.
Authors: Heider, H and Schroeder, C
Journal: Journal of virological methods (1997): 311-6

Histological and immunohistochemical diversities, and proliferative activity and grading in osteosarcomas.
Authors: Hasegawa, T and Hirose, T and Seki, K and Hizawa, K and Ishii, S and Wakabayashi, J
Journal: Cancer detection and prevention (1997): 280-7

Buccutite 聚HRP抗体偶联试剂盒

	货号	5518	存储条件	在2-8度冷藏保存, 避免光照
	规格	1×50 ug Labeling	价格	4740
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

在样品有限或目标分子含量极低的免疫测定中，聚-HRP-抗体结合物被认为具有最高的敏感性。 Buccutite 聚HRP抗体偶联试剂盒旨在快速制备聚HRP偶联抗体。该试剂盒提供了可通过我们专有的Buccutite FOL预激活的poly-HRP探针。用Buccutite MTA（试剂盒中提供）激活靶向抗体，以得到MTA激活的抗体。 MTA激活的抗体与FOL激活的多HRP的简单混合即可得到所需的多HRP标记的抗体偶联物。该反应在极温和的条件下进行而无需催化剂。用Buccutite Poly-HRP抗体偶联试剂盒制备的poly-HRP抗体偶联物与ELISA，western blotting，免疫组化（IHC）分析中使用的发色，荧光和化学发光HRP底物兼容。它也可以与TSA或我们的Styramide 荧光HRP底物一起使用，以超灵敏地检测低丰度生物靶标。

图示

图1.使用Buccutite Poly-HRP抗体偶联试剂盒（Cat＃5518或Cat＃5519）制备的polyHRP-山羊抗小鼠IgG偶联物生成直接ELISA曲线。将3倍系列稀释的小鼠IgG包被在96孔板上，并使用标准ELISA方法检测100uL GAM IgG-polyHRP缀合物（100ng / ml）。用TMB底物溶液（Cat＃11003）孵育5分钟检测固定的小鼠IgG，并在450 nm处读数。
蓝色：5518或5519试剂盒
红色：GXM IgG PolyHRP（供应商A）
绿色：GXM IgG-HRP（供应商B）

参考文献

Detecting cancer metastasis and accompanying protein biomarkers at single cell levels using a 3D-printed microfluidic immunoarray.
Authors: Sharafeldin, Mohamed and Chen, Tianqi and Ozkaya, Gulsum Ucak and Choudhary, Dharamainder and Molinolo, Alfredo A and Gutkind, J Silvio and Rusling, James F
Journal: Biosensors & bioelectronics (2021): 112681

Prostate Cancer Diagnosis in the Clinic Using an 8-Protein Biomarker Panel.
Authors: Jones, Abby L and Dhanapala, Lasangi and Baldo, Thaísa A and Sharafeldin, Mohamed and Krause, Colleen E and Shen, Min and Moghaddam, Shirin and Faria, Ronaldo C and Dey, Dipak K and Watson, R William and Andrawis, Ramez and Lee, Norman H and Rusling, James F
Journal: Analytical chemistry (2020)

Comparison of different methodologies and cryostat versus paraffin sections for chromogenic immunohistochemistry.
Authors: Hira, Vashendriya V V and de Jong, Annique Loncq and Ferro, Klea and Khurshed, Mohammed and Molenaar, Remco J and Van Noorden, Cornelis J F
Journal: Acta histochemica (2019): 125-134

The complementary role of insulin-like growth factor II mRNA-binding protein 3 (IMP3) in diagnosis of Hodgkin’s lymphoma.
Authors: Masoud, Rokia and Ibrahiem, Afaf and Tantawy, Dina and Eldosoky, Ibrahim
Journal: Annals of diagnostic pathology (2019): 64-68

Using magnetic beads and signal amplifiers to produce short and simple immunoassays: Application to MMP-9 detection in plasma samples.
Authors: Ben Ismail, Manel and de la Serna, Erica and Ruiz-Vega, Gisela and García-Berrocoso, Teresa and Montaner, Joan and Zourob, Mohammed and Othmane, Ali and Baldrich, Eva
Journal: Analytica chimica acta (2018): 144-154

Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) – β1 in urine.
Authors: Sánchez-Tirado, E and Martínez-García, G and González-Cortés, A and Yáñez-Sedeño, P and Pingarrón, J M
Journal: Biosensors & bioelectronics (2017): 9-14

Chemiluminometric Immunosensor for High-Sensitivity Cardiac Troponin I Employing a Polymerized Enzyme Conjugate as a Tracer.
Authors: Lim, Guei-Sam and Seo, Sung-Min and Paek, Sung-Ho and Kim, Seung-Wan and Jeon, Jin-Woo and Kim, Dong-Hyung and Cho, Il-Hoon and Paek, Se-Hwan
Journal: Scientific reports (2015): 14848

Design and construction of novel molecular conjugates for signal amplification (I): conjugation of multiple horseradish peroxidase molecules to immunoglobulin via primary amines on lysine peptide chains.
Authors: Dhawan, Subhash
Journal: Peptides (2002): 2091-8

Design and construction of novel molecular conjugates for signal amplification (II): use of multivalent polystyrene microparticles and lysine peptide chains to generate immunoglobulin-horseradish peroxidase conjugates.
Authors: Dhawan, Subhash
Journal: Peptides (2002): 2099-110

Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method.
Authors: Peterson, E M and Hughes, B L and Aarnaes, S L and de la Maza, L M
Journal: Journal of clinical microbiology (1988): 222-4