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磷酸基团绿色荧光底物 PhosLite
货号 | 11630 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 mg | 价格 | 2544 | |
Ex (nm) | 345 | Em (nm) | 520 | |
分子量 | 589.49 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:11630
产品名称:磷酸基团绿色荧光底物 PhosLite
规格:1mg
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:589.49
溶剂:水
激发波长(nm):345
发射波长(nm):520
产品介绍
磷酸基团绿色荧光底物 PhosLite 是美国AAT Bioquest生产的用于磷酸的试剂盒,这种水溶性PhosLite 绿色基质在磷酸酶活性位点产生明亮且光稳定的黄绿色荧光沉淀。其荧光沉淀具有几种独特的性质(例如极大的斯托克斯位移和高光稳定性),使该底物成为5-溴-4-氯-3-吲哚基磷酸酯(BCIP)的优异荧光形式,BCIP是用于敏感比色检测碱性磷酸酶活性。它可用于免疫印迹,原位杂交和免疫组织化学,但不需要加入硝基蓝四唑氯化物(NBT)。碱性磷酸酶通常与二抗结合。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的磷酸基团绿色荧光底物 PhosLite 。
参考文献
The spatial distribution of acid phosphatase activity in ectomycorrhizal tissues depends on soil fertility and morphotype, and relates to host plant phosphorus uptake
Authors: Alvarez M, Huygens D, Diaz LM, Villanueva CA, Heyser W, Boeckx P.
Journal: Plant Cell Environ (2012): 126
A novel fluorescent transcriptional reporter for cell-based microarray assays
Authors: Redmond TM, Uhler MD.
Journal: Methods Mol Biol (2011): 41
Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: the importance of time kinetics in ELFA labeling
Authors: Duhamel S, Gregori G, Van Wambeke F, Nedoma J.
Journal: Cytometry A (2009): 163
Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays
Authors: Jun SB, Hynd MR, Dowell-Mesfin NM, Al-Kofahi Y, Roysam B, Shain W, Kim SJ.
Journal: J Neural Eng (2008): 203
Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats
Authors: Akiba Y, Mizumori M, Guth PH, Engel E, Kaunitz JD.
Journal: Am J Physiol Gastrointest Liver Physiol (2007): G1223
Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation
Authors: Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto T.
Journal: J Bone Miner Res (2006): 1012
Detecting the phosphate status of phytoplankton by enzyme-labelled fluorescence and flow cytometry
Authors: Dignum M, Hoogveld HL, Matthijs HC, Laanbroek HJ, Pel R.
Journal: FEMS Microbiol Ecol (2004): 29
Enzymatic activity of alkaline phosphatase inside protein and polymer structures fabricated via multiphoton excitation
Authors: Basu S, Campagnola PJ.
Journal: Biomacromolecules (2004): 572
Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode
Authors: Telford WG, Hawley TS, Hawley RG.
Journal: Cytometry A (2003): 48
Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics
Authors: Nedoma J, Strojsova A, Vrba J, Komarkova J, Simek K.
Journal: Environ Microbiol (2003): 462