APC (F-3)抗体 (PE 标记) APC (F-3) PE

APC (F-3)抗体 (PE 标记)

APC (F-3) PE

详细描述:
The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner’s syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extr

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记: PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-9998 PE APC (F-3)抗体 (PE 标记) 200 µg/ml 咨询客服

消泡剂 SI,PE-H,PE-M,PE-L Antifoam SI,PE-H,PE-M,PE-L

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

消泡剂 SI,PE-H,PE-M,PE-L消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L



特点


SI(Silicone)

  本产品是硅系消泡剂,同时具有非常优异的破泡性和抑泡性。因此在低浓度也可得到消泡效果。另外,添加到培养基时,具有浑浊少的特点。


PE-H(Polyether-High),PE-M(Polyether-Medium),PE-L(Polyether-Low)

  本产品是聚醚系消泡剂。可将细胞毒性抑制到非常低的程度,根据粘度的不同,分成PE-H,PE-M,PE-L三种,一般粘度越低分散性越好。


消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L

Antifoam SI

消泡剂SI

消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L

Antifoam PE-H

消泡剂PE-H

消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L

Antifoam PE-M

消泡剂PE-M

消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L

Antifoam PE-L

多醚型消泡剂


添加量


  对于细胞培养,添加适量本品用于确认各系统的最终浓度保持在 0.25~0.5%(1 L 培养基中含 0.25~0.5 mL)。



用途

  用于细菌、细胞等培养时消除泡沫。



参考数据


1. 消泡效果与浑浊(度)测定


方法

向小试管中注入 5 mL 培养基,剧烈振荡后,添加消泡剂。
测定添加后 10 秒钟,5分钟,15 分钟的气泡程度,以及当时的浑浊度。


1) BHIB(细菌培养用培养基) + 0.04%Tween20

添加消泡剂后的泡沫高度 单位(mm)


分类

消泡剂

稀释
\倍数

时间

×102

×103

×2×103

×4×103

×8×103

×104

×105

对照组

有机硅类

消泡剂
SI

添加后10秒

0

0

3

5

8

14

19

24

5分钟

0

0

0

0

0

0

3

20

15分钟

0

0

0

0

0

0

2

13

浑浊度

S公司产品

添加后10秒

0

2

8

11

13

17

18

22

5分钟

0

0

0

1

8

9

13

16

15分钟

0

0

0

0

8

9

13

13

浑浊度

聚醚类

消泡剂
PE-M

添加后10秒

5

8

10

12

15

19

19

20

5分钟

3

4

5

7

9

10

15

16

15分钟

2

3

4

6

8

10

13

15

浑浊度

Oil


2) MEM(细胞培养用培养基)+ 10%FCS(胎牛血清)

添加消泡剂后的泡沫高度 单位(mm)


分类

消泡剂

稀释
\倍数

时间

×102

×103

×2×103

×4×103

×8×103

×104

×105

对照组

有机硅类

消泡剂
SI

添加后10秒

0

0

0

2

5

10

31

40

5分钟

0

0

0

0

0

0

0

34

15分钟

0

0

0

0

0

0

0

33

浑浊度

S公司产品

添加后10秒

0

1

2

4

6

11

19

40

5分钟

0

0

0

0

2

3

19

31

15分钟

0

0

0

0

1

2

17

24

浑浊度

聚醚类

消泡剂
PE-M

添加后10秒

1

1.5

3

3

3

9

25

47

5分钟

0

0

1

1

2

3

20

41

15分钟

0

0

0

0

1

1

2.5

41

浑浊度

少有

2. 细胞毒性测试


细胞

 Vero 细胞的 subclone(O78株)

活力测定

 Alamar Blue1)

方法

 添加 Alamar Blue,测定 LD50。


以下图表为 LD50 时稀释倍数和添加后天数的关系。

消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L 消泡剂 SI,PE-H,PE-M,PE-L                              Antifoam SI,PE-H,PE-M,PE-L

注意:细胞毒性非常低,用于培养没有问题,可用于各系统的培养。

参考文献

1) Page B., Pagge M. and Noel, C. : Intern. J. Oncal., 3, 473 (1993)

产品编号 产品名称 产品规格 产品等级
016-17431 Antifoam SI 
消泡剂SI
100ml 生物化学用
018-17435 Antifoam SI 
消泡剂SI
500ml 生物化学用
010-17191 Antifoam PE-H 
消泡剂PE-H
100ml 生物化学用
012-17195 Antifoam PE-H 
消泡剂PE-H
500mL 生物化学用
010-17211 Antifoam PE-M 
消泡剂PE-M
100mL 生物化学用
012-17215 Antifoam PE-M 
消泡剂PE-M
500mL 生物化学用
013-17201 Antifoam PE-L 
多醚型消泡剂(多醚-低型)
100mL 生物化学用
015-17205 Antifoam PE-L 
多醚型消泡剂(多醚-低型)
500mL 生物化学用

Ankyrin G (463) 抗体 (PE 标记) Ankyrin G (463) PE

Ankyrin G (463) 抗体 (PE 标记)

Ankyrin G (463) PE

详细描述:
Members of the Ankyrin family of proteins mediate the attachment of integral membrane proteins to the cytoskeleton. ANK1, ANK2 and ANK3 genes encode for the proteins in this family, Ankyrin-1 (also designated Ankyrin R), Ankyrin B and Ankyrin G, respectiv

应用范围:WB, IP, IF, IHC(P); 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12719 PE Ankyrin G (463) 抗体 (PE 标记) 200 µg/ml 咨询客服

BMP-4 (3H2.3) 抗体 (PE 标记) BMP-4 (3H2.3) PE

BMP-4 (3H2.3) 抗体 (PE 标记)

BMP-4 (3H2.3) PE

详细描述:
Bone morphogenic proteins (BMPs) are members of the TGFβ superfamily. BMPs are involved in the induction of cartilage and bone formation. In vivo studies have shown that BMP-2 (also designated BMP-2A) and BMP-3 can independently induce cartilage formation

应用范围:WB, IP, IF; 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12721 PE BMP-4 (3H2.3) 抗体 (PE 标记) 200 µg/ml 咨询客服

PTH/PTHrP-R (3D1.1) 抗体 (PE 标记) PTH/PTHrP-R (3D1.1) PE

PTH/PTHrP-R (3D1.1) 抗体 (PE 标记)

PTH/PTHrP-R (3D1.1) PE

详细描述:
Parathyroid hormone (PTH), which is also designated parathyrin, is an 84 amino acid single chain peptide that functions to regulate calcium metabolism by raising blood levels of calcium through various mechanisms. PTH stimulates bone formation to increase

应用范围:WB, IP, IF, IHC(P), FCM; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12722 PE PTH/PTHrP-R (3D1.1) 抗体 (PE 标记) 200 µg/ml 咨询客服

IL-4 (HIL41) 抗体 (PE 标记) IL-4 (HIL41) PE

IL-4 (HIL41) 抗体 (PE 标记)

IL-4 (HIL41) PE

详细描述:
Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in imm

应用范围:WB, IP, IF, ELISA; 反应种属:human; Isotype:mouse monoclonal IgG2b; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12723 PE IL-4 (HIL41) 抗体 (PE 标记) 200 µg/ml 咨询客服

CLIP (cerCLIP.1) 抗体 (PE 标记) CLIP (cerCLIP.1) PE

CLIP (cerCLIP.1) 抗体 (PE 标记)

CLIP (cerCLIP.1) PE

详细描述:
Classical major histocompatibility (MHC) class II complexes are formed in the endoplasmic recticulum and consist of three invariant chains that associate with three class II-ab dimers. The invariant chains contain translocation signals that shuttle the co

应用范围:WB, IP, IF, IHC(P), FCM; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12725 PE CLIP (cerCLIP.1) 抗体 (PE 标记) 200 µg/ml 咨询客服

AP-2α (3B5) 抗体 (PE 标记) AP-2α (3B5) PE

AP-2α (3B5) 抗体 (PE 标记)

AP-2α (3B5) PE

详细描述:
AP-2 transcription factor family members include AP-2α, AP-2β and AP-2γ, which specifically bind to the DNA consensus sequence CCCCAGGC and initiate transcription of selected genes. AP-2, also known as ERF-1, plays a role in regulating estrogen receptor e

应用范围:WB, IP, IF, IHC(P); 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12726 PE AP-2α (3B5) 抗体 (PE 标记) 200 µg/ml 咨询客服

CD28 (37.51.1) 抗体 (PE 标记) CD28 (37.51.1) PE

CD28 (37.51.1) 抗体 (PE 标记)

CD28 (37.51.1) PE

详细描述:
T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and

应用范围:IP, IF, FCM; 反应种属:mouse; Isotype:hamster monoclonal IgG; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12727 PE CD28 (37.51.1) 抗体 (PE 标记) 200 µg/ml 咨询客服

CD47 (B6H12) 抗体 (PE 标记) CD47 (B6H12) PE

CD47 (B6H12) 抗体 (PE 标记)

CD47 (B6H12) PE

详细描述:
CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matr

应用范围:WB, IP, IF, IHC(P), FCM; 反应种属:m, r, h, primate; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12730 PE CD47 (B6H12) 抗体 (PE 标记) 200 µg/ml 咨询客服

CD47 (MIAP301) 抗体 (PE 标记) CD47 (MIAP301) PE

CD47 (MIAP301) 抗体 (PE 标记)

CD47 (MIAP301) PE

详细描述:
CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matr

应用范围:WB, IP, IF, FCM; 反应种属:mouse; Isotype:rat monoclonal IgG2a; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12731 PE CD47 (MIAP301) 抗体 (PE 标记) 200 µg/ml 咨询客服

Clathrin HC (TD.1) 抗体 (PE 标记) Clathrin HC (TD.1) PE

Clathrin HC (TD.1) 抗体 (PE 标记)

Clathrin HC (TD.1) PE

详细描述:
Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are englufed by clathrin-coated vesicles. Clathrin is composed of three h

应用范围:WB, IP, IF; 反应种属:broad species; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12734 PE Clathrin HC (TD.1) 抗体 (PE 标记) 200 µg/ml 咨询客服

Syntaxin 1 (HPC-1) 抗体 (PE 标记) Syntaxin 1 (HPC-1) PE

Syntaxin 1 (HPC-1) 抗体 (PE 标记)

Syntaxin 1 (HPC-1) PE

详细描述:
Correct vesicular transport is essential to the survival of eukaryotic cells. This process is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attac

应用范围:WB, IP, IF, IHC(P), ELISA; 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12736 PE Syntaxin 1 (HPC-1) 抗体 (PE 标记) 200 µg/ml 咨询客服

casein kinase IIβ (6D5) 抗体 (PE 标记) casein kinase IIβ (6D5) PE

casein kinase IIβ (6D5) 抗体 (PE 标记)

casein kinase IIβ (6D5) PE

详细描述:
Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Ie, hav

应用范围:WB, IP, IF, IHC(P); 反应种属:m, r, h; Isotype:mouse monoclonal IgG1; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12739 PE casein kinase IIβ (6D5) 抗体 (PE 标记) 200 µg/ml 咨询客服

Annexin I (EH17a) 抗体 (PE 标记) Annexin I (EH17a) PE

Annexin I (EH17a) 抗体 (PE 标记)

Annexin I (EH17a) PE

详细描述:
The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding spe

应用范围:WB, IP, IF, IHC(P); 反应种属:m, r, h; Isotype:mouse monoclonal IgG2b; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12740 PE Annexin I (EH17a) 抗体 (PE 标记) 200 µg/ml 咨询客服

IL-1α (ALF-161) 抗体 (PE 标记) IL-1α (ALF-161) PE

IL-1α (ALF-161) 抗体 (PE 标记)

IL-1α (ALF-161) PE

详细描述:
Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. I

应用范围:WB, IP, IF, FCM, ELISA; 反应种属:m, r, h; Isotype:hamster monoclonal IgG; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12741 PE IL-1α (ALF-161) 抗体 (PE 标记) 200 µg/ml 咨询客服

IL-1β (B122) 抗体 (PE 标记) IL-1β (B122) PE

IL-1β (B122) 抗体 (PE 标记)

IL-1β (B122) PE

详细描述:
Two forms of interleukin-1, designated IL-1α and IL-1β, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1α and IL-1β bind to the same receptor and seem to elicit similar biological responses. I

应用范围:WB, IP, ELISA; 反应种属:m, r, h, hamster; Isotype:hamster monoclonal IgG; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12742 PE IL-1β (B122) 抗体 (PE 标记) 200 µg/ml 咨询客服

TNFα (TN3-19.12) 抗体 (PE 标记) TNFα (TN3-19.12) PE

TNFα (TN3-19.12) 抗体 (PE 标记)

TNFα (TN3-19.12) PE

详细描述:
Tumor necrosis factor β (TNFβ), also known as lymphotoxin, is a pleiotropic cytokine. TNFα, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNFβ. TNFβ and TNFα share 30

应用范围:WB, IP, IF; 反应种属:m, r, rabbit; Isotype:hamster monoclonal IgG; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12744 PE TNFα (TN3-19.12) 抗体 (PE 标记) 200 µg/ml 咨询客服

TNF-R1 (55R-170) 抗体 (PE 标记) TNF-R1 (55R-170) PE

TNF-R1 (55R-170) 抗体 (PE 标记)

TNF-R1 (55R-170) PE

详细描述:
Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types. The majority of TNF functions are primarily medi

应用范围:IP, FCM; 反应种属:m, r, h; Isotype:hamster monoclonal IgG; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12746 PE TNF-R1 (55R-170) 抗体 (PE 标记) 200 µg/ml 咨询客服

TNF-R2 (TR75-89) 抗体 (PE 标记) TNF-R2 (TR75-89) PE

TNF-R2 (TR75-89) 抗体 (PE 标记)

TNF-R2 (TR75-89) PE

详细描述:
Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types. The majority of TNF functions are primarily medi

应用范围:WB, IP, IF, FCM; 反应种属:m, r, h; Isotype:hamster monoclonal IgG; 标记:PE。

货号 产品名称 品牌 购买
货号 名称 单位 购买
sc-12750 PE TNF-R2 (TR75-89) 抗体 (PE 标记) 200 µg/ml 咨询客服