钙黄绿素绿,AM 货号21905-AAT Bioquest荧光染料

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钙黄绿素绿,AM

钙黄绿素绿,AM

钙黄绿素绿,AM    货号21905 货号 21905 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 2544
Ex (nm) 492 Em (nm) 514
分子量 607.47 溶剂 DMSO
产品详细介绍

简要概述

钙黄绿素绿,AM是美国AAT Bioquest生产的用于检测活细胞的荧光探针,Calcein UltraGreen AM可轻松通过活细胞的细胞膜。在运输到活细胞中时,细胞酯酶切断了AM基团,该分子被捕获在细胞内。与Calcein AM相比,Calcein UltraGreen 更适合用于染色活细胞的荧光探针,因为它具有较低的细胞毒性和较长的细胞保留时间。UltraGreen AM不会显着影响细胞功能,如淋巴细胞的增殖或趋化性。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙黄绿素绿,AM。 

点击查看实验方案

 

适用仪器


流式细胞仪  
激发: 488nm激光
发射: 530/30nm滤波片
推荐孔板: FITC通道
荧光显微镜  
激发: FITC
发射: FITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 490nm
发射: 525nm
cutoff: 515nm
推荐孔板: 黑色孔板
读取模式: 底读模式

产品说明书

操作步骤

1.准备HHBS缓冲液,10%Pluronic®F-127溶液和25 mM Probenecid溶液。

2.在高质量无水DMSO中制备2 mM至5 mM Calcein UltraGreen AM原液。
2.1使用Calcein UltraGreen AM的量:1毫克
2.2所需浓度:2 mM
2.3在合适的容器中,将1mg钙黄绿素UltraGreen TM AM与823.09μL无水DMSO混合。
 

3.使用10μMCalceinUltraGreen AM 4在HHBS中制备2X工作溶液,0.08%Pluronic®F-127和2 mM丙磺舒。
3.1最终孔内浓度的Calcein UltraGreen AM:5μM
3.2Pluronic®F-127的最终孔内浓度:0.04%
3.3最终孔内浓度的丙磺舒:1mM
3.4在合适的容器中混合16μL的Calcein UltraGreen AM,25.6μL的10%Pluronic®F-127和256μL的25 mM丙磺舒。然后,添加HHBS或您选择的缓冲液,直到体积为3.2 mL。
注意:对于大多数细胞系,我们建议Calcein UltraGreen AM的最终浓度为4至5μM。
注意:推荐的Pluronic F-127井浓度最终为0.02%至0.04%。
注意:推荐的最终浓度为1至2.5 mM的Probenecid。
 

4.将100μL染料工作溶液加入已经含有100μL培养基的所需孔中。
4.1该步骤将染料工作溶液从2X稀释至1X,并将每种组分的最终浓度调节至以下:5μM钙黄绿素UltraGreen AM,0.04%Pluronic®F-127,1mM丙磺舒。

 

5.孵育染料
5.1将染料加载板在细胞培养箱中孵育20-60分钟。
5.2将染料加载板在室温下孵育30分钟。
 

6.用1.0 mM Probenecid准备HHBS缓冲液(或您选择的缓冲液)。
6.1在合适的容器中加入160μL的25mM丙磺舒。 接下来,添加HHBS或您选择的缓冲液,直到体积为4 mL。
 

7.用HHBS缓冲液或您选择的缓冲液替换染料工作溶液,使用1.0 mM Probenecid。
7.1首先,从所需孔中除去200μL染料工作溶液和培养基。
7.2在相同的孔中加入200μL含有1.0mM丙磺舒的HHBS(或您选择的缓冲液)。
 

8.运行实验
8.1为您的样品添加所需的处理。
8.2以Ex / Em = 496/524 nm运行实验。

 

参考文献

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599–2610

NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal: Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal: MEDICINE (2016): 1–8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal: Tissue Engineering Part C: Methods (2016): 322–331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81–88

Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1–8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)

Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Anja Lena Thiebes, Stefanie Albers, Christian Klopsch, Stefan Jockenhoevel, Christian G Cornelissen
Journal: BioResearch open access (2015): 278–287

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property
Authors: Shuai Wu, Zhuoyao Huang, Jianhui Yue, Di Liu, Ting Wang, Pierre Ezanno, Changshun Ruan, Xiaoli Zhao, William W Lu, Haobo Pan
Journal: Carbohydrate polymers (2015): 295–303

 

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说明书
钙黄绿素绿,AM.pdf